Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 17,334 results for: form

Showing: 8441 - 8460 of 17334 results

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pGL4_EGR1p-BC1484-luc2

Plasmid

#227138

Purpose

Barcoded assay, MAPK sensor; barcode BC1484

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

EGR1 promoter driving barcode 1484 and a luciferase reporter gene

Expression

Mammalian

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4_EGR1p-BC0136-luc2

Plasmid

#227137

Purpose

Barcoded assay, MAPK sensor; barcode BC0136

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

EGR1 promoter driving barcode 0136 and a luciferase reporter gene

Expression

Mammalian

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pRGEB32-CrU6-2-35S

Plasmid

#226706

Purpose

For CRISPR/Cas-mediated gene editing in model fern species, Ceratopteris richardii; expresses guide RNAs from CrU6-2 promoter and Cas9 from CaMV 35S promoter.

Depositor

Fenglin Deng

Article

Jiang et al Plant Physiol. 2024 Sep 12:kiae473. doi:

Insert

CrU6-2

Use

CRISPR

Available Since

Nov. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pRGEB32-CrActin-35S

Plasmid

#226708

Purpose

For CRISPR/Cas-mediated gene editing in model fern species, Ceratopteris richardii; expresses guide RNAs from CrActin promoter and Cas9 from CaMV 35S promoter.

Depositor

Fenglin Deng

Article

Jiang et al Plant Physiol. 2024 Sep 12:kiae473. doi:

Insert

CrActin

Use

CRISPR

Available Since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pRGEB32-CrActin-UBI

Plasmid

#226709

Purpose

For CRISPR/Cas-mediated gene editing in model fern species, Ceratopteris richardii; expresses guide RNAs from CrActin promoter and Cas9 from maize ubiquitin (ZmUbi) promoter.

Depositor

Fenglin Deng

Article

Jiang et al Plant Physiol. 2024 Sep 12:kiae473. doi:

Insert

CrActin

Use

CRISPR

Available Since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pRGEB32-CrU6-2-UBI

Plasmid

#226707

Purpose

For CRISPR/Cas-mediated gene editing in model fern species, Ceratopteris richardii; expresses guide RNAs from CrU6-2 promoter and Cas9 from maize ubiquitin (ZmUbi) promoter.

Depositor

Fenglin Deng

Article

Jiang et al Plant Physiol. 2024 Sep 12:kiae473. doi:

Insert

CrU6-2

Use

CRISPR

Available Since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL4_EGR1p-BC0132-luc2

Plasmid

#227136

Purpose

Barcoded assay, MAPK sensor; barcode BC0132

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

EGR1 promoter driving barcode 0132 and a luciferase reporter gene

Expression

Mammalian

Available Since

Nov. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28:LanIV-Streptomyces

Plasmid

#226250

Purpose

Expresses precursor peptide and lanthipeptide synthetase from Streptomyces LanIV (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanIV-Streptomyces)

Tags

His6

Expression

Bacterial

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-mGFP-FXR1a-Ccswap

Plasmid

#225460

Purpose

Express EGFP-fusion protein of FXR1 isoform a (CCswap)

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

FXR1 isoform a (FXR1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

CCswap (swapped positions of CC2 and CC1)

Promoter

CMV

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-mGFP-FXR1a-CC1-CC1

Plasmid

#225458

Purpose

Express EGFP-fusion protein of FXR1 isoform a (CC1-CC1)

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

FXR1 isoform a (FXR1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

CC2 replaced with a second copy of CC1

Promoter

CMV

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-mGFP-FXR1-N1-KH1mut (T236D H237D)

Plasmid

#225453

Purpose

Express mEGFP-fusion protein of FXR1 isoform a (T236D H237D)

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

FXR1 isoform a (FXR1 Human)

Tags

mEGFP

Expression

Mammalian

Mutation

T236D H237D

Promoter

CMV

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-mGFP-FXR1a-CC2mut (V361P)

Plasmid

#225456

Purpose

Express mEGFP-fusion protein of FXR1 isoform a (V361P)

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

FXR1 isoform a (FXR1 Human)

Tags

mEGFP

Expression

Mammalian

Mutation

V361P

Promoter

CMV

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-mGFP-FXR1a-CC2-CC2

Plasmid

#225459

Purpose

Express mEGFP-fusion protein of FXR1 isoform a (CC2-CC2)

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

FXR1 isoform a (FXR1 Human)

Tags

mEGFP

Expression

Mammalian

Mutation

CC1 replaced with a second copy of CC2

Promoter

CMV

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28:LanII-56

Plasmid

#225073

Purpose

Expresses precursor peptide and lanthipeptide synthetase from LanII-56 (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanII-56)

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pET28:LanII-2C

Plasmid

#225070

Purpose

Expresses precursor peptide and lanthipeptide synthetase from LanII-2C (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanII-2C)

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28:LanII-2A

Plasmid

#225068

Purpose

Expresses precursor peptide and lanthipeptide synthetase from LanII-2A (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanII-2A)

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28:LanII-2B

Plasmid

#225069

Purpose

Expresses precursor peptide and lanthipeptide synthetase from LanII-2B (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanII-2B)

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28:LanII-57

Plasmid

#225074

Purpose

Expresses two precursor peptides and the lanthipeptide synthetase from LanII-57 (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA(A) (LanII-57)

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28:LanIII-6

Plasmid

#225076

Purpose

Expresses precursor peptide and lanthipeptide synthetase from LanIII-6 (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA(A) (LanIII-6)

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28:LanII-2E

Plasmid

#225072

Purpose

Expresses precursor peptide and lanthipeptide synthetase from LanII-2E (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanII-2E)

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

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