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pML107-LUG1

Plasmid

#226265

Purpose

Plasmid expressing Cas9 and gRNA TCTTCAAGTTACTCCAAGAG which targets the LUG1 gene

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

LUG1 gRNA (YLR352W Budding Yeast)

Use

CRISPR

Expression

Yeast

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pML104-LEU2-2

Plasmid

#226263

Purpose

Plasmid expressing Cas9 and gRNA GGTAGTGTTAGACCTGAACA which targets the LEU2 gene

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

LEU2 gRNA (LEU2 Budding Yeast)

Use

CRISPR

Expression

Yeast

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pRS313-SCT1-NCYC110

Plasmid

#226269

Purpose

Plasmid expressing the SCT1 allele from NCYC110, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SCT1 (SCT1 Budding Yeast)

Expression

Yeast

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pRS313-SCT1-UWOPS872421

Plasmid

#226268

Purpose

Plasmid expressing the SCT1 allele from UWOPS87-2421, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SCT1 (SCT1 Budding Yeast)

Expression

Yeast

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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gRNA_CloningVector_tagBFP

Plasmid

#163747

Purpose

Empty gRNA cloning vector, contains a tagBFP fluorescent marker for FACS sorting after transfection.

Depositor

Jennifer Mitchell

Article

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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gRNA_CloningVector_miRFP670

Plasmid

#163748

Purpose

Empty gRNA cloning vector, contains a miRFP670 fluorescent marker for FACS sorting after transfection.

Depositor

Jennifer Mitchell

Article

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pTE5417_p3WJdB_promoter dropout vector

Plasmid

#221191

Purpose

Promoter probe vector with dropout sequence for promoter sequences with 3WJdB reporter

Depositor

Tobias Erb

Article

Barthel et al Synth Biol (Oxf). 2024 Sep 20;9(1):ysae0

Type

Empty backbone

Use

Synthetic Biology

Promoter

Promoter Placeholder/Dropout Vector

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pPOTvGPI(EP)-blast-mNG

Plasmid

#221051

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mNeonGreen

Use

Unspecified

Promoter

read through

Available Since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pPOTvGPI(VSG)-blast-mNG

Plasmid

#221050

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mNeonGreen

Use

Unspecified

Promoter

read through

Available Since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pSM045

Plasmid

#217399

Purpose

KmR, pBTRcK broad host range vector with BTE1(C12-specific fatty acid thioesterase codon-optimised for C. necator) under Ptrc

Depositor

Brian Pfleger

Article

Mishra et al J Ind Microbiol Biotechnol. 2024 Feb 16:

Insert

BTE1

Expression

Bacterial

Promoter

PTrc

Available Since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSCL194

Plasmid

#222225

Purpose

Expresses site 1/Cas9/Eco1 RT in HIS3 locus.

Depositor

Seth Shipman

Article

Crawford et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

Insert

site 1/Cas9/Eco1 RT

Use

CRISPR

Expression

Yeast

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pSCL195

Plasmid

#222226

Purpose

Expresses site 2/Cas9/Eco1 RT in HIS3 locus.

Depositor

Seth Shipman

Article

Crawford et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

Insert

site 2/Cas9/Eco1 RT

Use

CRISPR

Expression

Yeast

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pSCL368

Plasmid

#222227

Purpose

Expresses site 3/Cas9/Eco1 RT in HIS3 locus.

Depositor

Seth Shipman

Article

Crawford et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

Insert

site 3/Cas9/Eco1 RT

Use

CRISPR

Expression

Yeast

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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T. aq sigmaA

Plasmid

#215350

Purpose

Express T. aq sigmaA transcription initiation factor in E. coli cells

Depositor

Julius Lucks

Article

Hertz et al Nucleic Acids Res. 2024 May 8;52(8):4466

Insert

rpoD

Tags

6x His

Expression

Bacterial

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pAcGFP-N1_CBPdIDR7dAL

Plasmid

#221914

Purpose

Transient expression of GFP-tagged CBPdeltaIDR7deltaAL in mammalian cells

Depositor

Daniel Bose

Insert

CBP (CREBBP Human)

Tags

GFP

Expression

Mammalian

Mutation

deletion of IDR7 (aa 2109-2434); deletion of the …

Available Since

June 28, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-H2B-MCS

Plasmid

#184041

Purpose

Cloning of GFP-H2B fusion proteins

Depositor

Lienhard Schmitz

Article

Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

Type

Empty backbone

Tags

GFP and H2B

Expression

Mammalian

Promoter

CMV

Available Since

June 10, 2024

Availability

Academic Institutions and Nonprofits only

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pSM046

Plasmid

#217400

Purpose

KmR, pBTRcK broad host range vector with BTE2(C12-specific fatty acid thioesterase codon-optimised for C. necator) under Ptrc

Depositor

Brian Pfleger

Article

Mishra et al J Ind Microbiol Biotechnol. 2024 Feb 16:

Insert

BTE2

Use

Synthetic Biology

Expression

Bacterial

Promoter

PTrc

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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pC0.425

Plasmid

#203944

Purpose

Up Flank. Up flanking region of RSF1010-Cas12a vector for introducing gRNA expression cassette and swapping out the AbR.

Depositor

Alistair McCormick

Article

Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

Insert

Cas12a UP

Use

Synthetic Biology

Available Since

May 10, 2024

Availability

Academic Institutions and Nonprofits only

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pC0.426

Plasmid

#203945

Purpose

Down Flank. Down flanking region of RSF1010-Cas12a vector for introducing gRNA expression cassette and swapping out the AbR.

Depositor

Alistair McCormick

Article

Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

Insert

Cas12a DOWN

Use

Synthetic Biology

Available Since

May 10, 2024

Availability

Academic Institutions and Nonprofits only

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pC0.391

Plasmid

#203948

Purpose

Level 0 part. Cas12a coding sequence from Francisella novicida in CDS1 position

Depositor

Alistair McCormick

Article

Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

Insert

FnCas12a

Use

Synthetic Biology

Available Since

May 10, 2024

Availability

Academic Institutions and Nonprofits only

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