We narrowed to 13,908 results for: RING;
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Plasmid#91023PurposeModule A, Promoter: AtUbi10, Gene: Csy4-P2A-AtCas9_dead (D10A + H840A), Terminator: HSPDepositorInsertCsy4-P2A-AtCas9_dead (D10A + H840A)
UseCRISPRTagsExpressionMutationD10A, H840APromoterAtUbi10Available SinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
TB:2xP53_RE:MiniP
Plasmid#133881Purpose2 copies of the p53 DNA binding motifDepositorInsert2 copies of the p53 DNA binding motif
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable SinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
Non-targeting CRISPRi plasmid
Plasmid#154891PurposeHuman non-targeting CRISPRi gRNADepositorInsertNontargeting gRNA
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable SinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAtCas9-dead_1
Plasmid#91165PurposeCas9 only expression, Cas9 Type: AtCas9_D10A+H840A (dead), Plant Selection: 2x35S:hpt IIDepositorInsertAtCas9_D10A+H840A (dead)
UseCRISPRTagsExpressionPlantMutationD10A, H840APromoterAvailable SinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
SEPLuc-IRES-Antares
Plasmid#141085Purposeprecursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosisDepositorInsertSEPLuc-EMCV IRES-Antares
UseTagsExpressionMammalianMutationPromoterCMVAvailable SinceMay 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNB655
Plasmid#60142PurposeFirefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by MET17 promoter.DepositorInsertFLuc-yEVenus
UseTagsExpressionYeastMutationA4VPromoterMET17prAvailable SinceFeb. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMDJ16 - pEXP[Peft-3 | mMaple3 (NLS) | tbb-2 UTR]
Plasmid#159802PurposeExpression construct with ubiquitous expression to test fluorescence level on microscopeDepositorInsertpEXP[Peft-3 | mMaple3 (NLS) | tbb-2 UTR]
UseTagsExpressionWormMutationNot applicablePromoterAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJG493
Plasmid#91197PurposeWDV replicon T-DNA for gene targeting in wheat scutella, no Cas9 control (gUbi1+gUbi8+donor)DepositorInsertgUbi1+gUbi8+donor
UseCRISPRTagsExpressionPlantMutationPromoterAvailable SinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
STAGR_SAMScaffold_h7SK
Plasmid#102846PurposeCan be used as PCR template for a STAgR reactionDepositorInsertSTAgR Insert SAM_h7SK
UsePcr template for stagr insertsTagsExpressionMutationPromoterH7SKAvailable SinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB106-OEP7(1–50)
Plasmid#100602PurposeBinary vector for expression of Citrine-fused AtOEP7(1-50) (OEP7(1–50)-Citrine) to visualize chloroplast outer envelope membrane in plantsDepositorAvailable SinceFeb. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET29b-AviTag-eGFP-dspB(E184Q)-6xHis
Plasmid#175784PurposePlasmid for overexpression of recombinant AviTagged, His-tagged fusion protein eGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.DepositorInsertDispersin B
UseTagsAviTag, Hexahistidine tag, and eGFPExpressionBacterialMutationE184QPromoterT7Available SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0112
Plasmid#117548PurposeLevel 1 Golden Gate Cassette: xCas9 3.7 expression cassette for dicotyledonous plantsDepositorInsert2x35S+5'UTR OMEGA (pICH51288) + Sp-xCas9 3.7 (no stop codon) (pEPOR0SP0011) + YFP (pICSL50005) + Nos (pICH41421)
UseTagsExpressionPlantMutationPromoter2x35S+5'UTR OMEGA (pICH51288)Available SinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBS-TRE-cofilin1(S3A)-P2A-mTurquoise2
Plasmid#163610PurposeExpresses constitutively active cofilin and mTurquoise2 under the TRE promoter in mammalian cellsDepositorAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
JARID2-KO-gRNA-1
Plasmid#131328PurposegRNA to knockout JARID2. Use with JARID2-KO-gRNA-2DepositorInsertJARID2-KO-gRNA-1
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable SinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
JARID2-KO-gRNA-2
Plasmid#131327PurposegRNA to knockout JARID2. Use with JARID2-KO-gRNA-1DepositorInsertJARID2-KO-gRNA-2
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable SinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
JM3 [JBEI-6891]
Plasmid#89788PurposeBroad host range vector pBBR1-MCS2 containing 'tesA (E. coli), fadB (E. coli), Mlut_11700 (M. luteus) and fadM (E. coli) under control of BAD promoterDepositorInserttesA (E. coli), fadB (E. coli), Mlut_11700 (M. luteus) and fadM (E. coli)
UseTagsExpressionBacterialMutationPromoterAvailable SinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTaCas9_5
Plasmid#91168PurposeCas9 only expression, Cas9 Type: TaCas9, Plant Selection: PvUbi2:hpt IIDepositorInsertTaCas9
UseCRISPRTagsExpressionPlantMutationPromoterAvailable SinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAtCas9-H840A_2
Plasmid#91163PurposeCas9 only expression, Cas9 Type: AtCas9_H840A (nickase), Plant Selection: 2x35S:npt IIDepositorInsertAtCas9_H840A (nickase)
UseCRISPRTagsExpressionPlantMutationH840APromoterAvailable SinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET22b-ecDHFR-G51PEKN
Plasmid#118582PurposeBacterial expression of E.coli DHFR G51PEKN mutantDepositorInsertDHFR
UseTagsExpressionBacterialMutationG51 mutated to P51; Glu-Lys-Asn residues inserted…PromoterT7Available SinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2262A - [1-2] ENTR - Fluor - ce-mNeon (1 intron, syntrons(3), no_atg, no_stop)
Plasmid#159851PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - ce-mNeon (1 intron, syntrons(3), no_atg, no_stop)
UseTagsExpressionWormMutationNot applicablePromoterAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only