We narrowed to 9,182 results for: Pol

1. pK RagA-RagC(K84T)

   Plasmid

   #99708

   Purpose

   Bacterial expression of RagA-RagC(K84T)

   Depositor

   David Sabatini, Kuang Shen

   Article

   Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

   Inserts

   RagA (RRAGA Human)
   RagC (RRAGC Human)
   

   Tags

   His tag, Poly Arg, and SUMO

   Expression

   Bacterial

   Mutation

   K84T

   Promoter

   T7

   Available Since

   Nov. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pK RagA(T21N)-RagC

   Plasmid

   #99650

   Purpose

   Bacterial expression of RagA(T21N)-RagC

   Depositor

   David Sabatini, Kuang Shen

   Article

   Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

   Inserts

   RagA (RRAGA Human)
   RagC (RRAGC Human)
   

   Tags

   His tag, Poly Arg, and SUMO

   Expression

   Bacterial

   Mutation

   T21N

   Available Since

   Nov. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pK RagA-RagC(S75N)

   Plasmid

   #99699

   Purpose

   Bacterial expression of RagA-RagC(S75N)

   Depositor

   David Sabatini, Kuang Shen

   Article

   Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

   Inserts

   RagA (RRAGA Human)
   RagC (RRAGC Human)
   

   Tags

   His tag, Poly Arg, and SUMO

   Expression

   Bacterial

   Mutation

   S75N

   Available Since

   Nov. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFastBac1-OSF-TRIMCyp (owl monkey)

   Plasmid

   #79035

   Purpose

   Baculoviral entry vector to produce Bac-to-bac baculoviruses

   Depositor

   Wesley Sundquist

   Article

   Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

   Insert

   OSF-TRIM5Cyp (owl monkey)
   

   Tags

   OneSTrEP-FLAG

   Expression

   Insect

   Promoter

   polyhedrin

   Available Since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGS-3R112C/T180C

   Plasmid

   #85095

   Purpose

   Host vector to fuse a gene at the N-terminus of PCNA3 variant (R112C/T180C)

   Depositor

   Teruyuki Nagamune

   Article

   Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

   Insert

   DNA polymerase sliding clamp 3
   

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   changed Arginine 112 to Cysteine and Threonine 18…

   Promoter

   T7 promoter

   Available Since

   Nov. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB-TAC-WTSI

   Plasmid

   #80486

   Purpose

   piggyBac transposon for dox-inducible expression of the WTSI polycistronic reprogramming cassette (with mCherry)

   Depositor

   Knut Woltjen

   Article

   Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

   Insert

   WTSI
   

   Use

   Piggybac transposon

   Expression

   Mammalian

   Promoter

   tetO

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. JARID1BA-c539

   Plasmid

   #79643

   Purpose

   Baculovirus expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   JARID1B (KDM5B Human)
   

   Use

   Baculovirus expression

   Tags

   6x His and TEV cleavage site

   Promoter

   polyhedrin

   Available Since

   June 16, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pET23a-MmUBB(SnaBI)

   Plasmid

   #69558

   Purpose

   In vitro transcription/translation (T7)

   Depositor

   Jorge Eduardo Azevedo

   Article

   Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

   Insert

   UBB (Ubb Mouse)
   

   Expression

   Bacterial

   Mutation

   The two last codons (TATTAA; encoding the last ty…

   Promoter

   T7

   Available Since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pET23a-MmUBB(Y305P)

   Plasmid

   #69557

   Purpose

   Bacterial expression; in vitro transcription/translation (T7)

   Depositor

   Jorge Eduardo Azevedo

   Article

   Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

   Insert

   UBB (Ubb Mouse)
   

   Expression

   Bacterial

   Mutation

   Codon TAT encoding tyrosine 305 was changed to CC…

   Promoter

   T7

   Available Since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pET15b+PCNA1 G108C/P186C

    Plasmid

    #66174

    Purpose

    Expresses the G108C/P186C mutant of Sulfolobus solfataricus PCNA1

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

    Insert

    DNA polymerase sliding clamp 1
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    G108C, P186C

    Promoter

    T7 promoter

    Available Since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMuLE ENTR 7SK R4-R3

    Plasmid

    #62109

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter for shRNA expression. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Type

    Empty backbone

    Use

    RNAi; Mule gateway entry vector

    Expression

    Mammalian

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMuLE ENTR 7SK L5-L2

    Plasmid

    #62108

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter for shRNA expression. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Type

    Empty backbone

    Use

    RNAi; Mule gateway entry vector

    Expression

    Mammalian

    Available Since

    Feb. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMuLE ENTR 7SK L5-L4

    Plasmid

    #62107

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter for shRNA expression. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Type

    Empty backbone

    Use

    RNAi; Mule gateway entry vector

    Expression

    Mammalian

    Available Since

    Feb. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMuLE ENTR 7SK L1-R5

    Plasmid

    #62106

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter for shRNA expression. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Type

    Empty backbone

    Use

    RNAi; Mule gateway entry vector

    Expression

    Mammalian

    Available Since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFSW Syt1-C2A K189-192A IRES GFP

    Plasmid

    #133823

    Purpose

    Encodes the C2A domain of synaptotagmin 1 with poly-lysine patch mutations K189-192A for viral expression

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

    Insert

    Syt1 (Syt1 Rat)
    

    Use

    Lentiviral

    Tags

    IRES-GFP

    Mutation

    K189-192A

    Promoter

    hSyn (human synapsin I promoter)

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLIB-GST-TEV-RSP3(160-516)

    Plasmid

    #176692

    Purpose

    Expression of Chlamydomonas reinhardtii RSP3 recombinant protein in insect cells

    Depositor

    Ron Vale

    Article

    Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

    Insert

    RSP3
    

    Tags

    GST

    Expression

    Insect

    Mutation

    None

    Promoter

    polyhedrin

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFastBac(His10-Lckg2a,Y394F)

    Plasmid

    #177893

    Purpose

    Baculo expression of His10 tag fused to human LckG2A

    Depositor

    Ron Vale

    Article

    Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

    Insert

    Lck (LCK Human)
    

    Tags

    10xHis-TEV

    Expression

    Insect

    Mutation

    G2A,Y394F

    Promoter

    Polyhedrin

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHCMM1

    Plasmid

    #167452

    Purpose

    Expresses C.elegans PUP-2 linked to RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (+PAB)] from the TEF1 promoter

    Depositor

    Marvin Wickens, Hugo Cesar Medina-Munoz

    Article

    Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

    Insert

    PTEF1_yeGFP_scPAB14RRMS_cePUP-2_URA3_TADH1
    

    Expression

    Yeast

    Promoter

    TEF1

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHCMM2

    Plasmid

    #167453

    Purpose

    Expresses C.elegans PUP-2 linked to RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (+PAB)] from the Sec63 promoter

    Depositor

    Marvin Wickens, Hugo Cesar Medina-Munoz

    Article

    Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

    Insert

    PSEC63_yeGFP_scPAB14RRMS_cePUP-2_URA3_TADH1
    

    Expression

    Yeast

    Promoter

    PSEC63

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHCMM3

    Plasmid

    #167454

    Purpose

    A control chimera; expresses C. elegans PUP-2 from the Sec63 promoter without the RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (-PAB)]

    Depositor

    Marvin Wickens, Hugo Cesar Medina-Munoz

    Article

    Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

    Insert

    PSEC63_yeGFP_cePUP-2_URA3_TADH1
    

    Expression

    Yeast

    Promoter

    PSEC63

    Availability

    Academic Institutions and Nonprofits only

    

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