We narrowed to 13,995 results for: eng
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Plasmid#228651PurposeBacterial expression of the fusion of the NS2B-NS3 protease from Dengue virus serotype 3 for biochemical assaysDepositorInsertDengue 3 portion of NS2B protease fused to NS3
TagsHis-Twin Strep-TevExpressionBacterialAvailable SinceFeb. 18, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
DENV-2 strain Thailand/16681/84 NS2B-NS3 protease fusion with non cleavable linker
Plasmid#228650PurposeBacterial expression of the fusion of the NS2B-NS3 protease from Dengue virus serotype 2 for biochemical assaysDepositorInsertDengue 2 portion of NS2B protease fused to NS3
TagsHis-Twin Strep-TevExpressionBacterialAvailable SinceFeb. 18, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
DENV-1 strain US/Hawaii/1944 NS2B-NS3 protease fusion with non cleavable linker
Plasmid#228648PurposeBacterial expression of the fusion of the NS2B-NS3 protease from Dengue virus serotype 1 for biochemical assayDepositorInsertDengue 1 portion of NS2B protease fused to NS3
TagsHis-Twin Strep-TevExpressionBacterialAvailable SinceFeb. 18, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
TMD18
Plasmid#163673PurposeExpresses a ST6GAL1 mutant with 18 amino acids in its TMD in mammalian cellsDepositorInsertpartial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)
TagsGFPExpressionMammalianMutationFor this partial length ST chimera, two restricti…Available SinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
TMD19
Plasmid#163674PurposeExpresses a ST6GAL1 mutant with 19 amino acids in its TMD in mammalian cellsDepositorInsertpartial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)
TagsGFPExpressionMammalianMutationFor this partial length ST chimera, two restricti…Available SinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
TMD20
Plasmid#163675PurposeExpresses a ST6GAL1 mutant with 20 amino acids in its TMD in mammalian cellsDepositorInsertpartial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)
TagsGFPExpressionMammalianMutationFor this partial length ST chimera, two restricti…Available SinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
TMD21
Plasmid#163676PurposeExpresses a ST6GAL1 mutant with 21 amino acids in its TMD in mammalian cellsDepositorInsertpartial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)
TagsGFPExpressionMammalianMutationFor this partial length ST chimera, two restricti…Available SinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
TMD22
Plasmid#163677PurposeExpresses a ST6GAL1 mutant with 22 amino acids in its TMD in mammalian cellsDepositorInsertpartial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)
TagsGFPExpressionMammalianMutationFor this partial length ST chimera, two restricti…Available SinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLY54
Plasmid#130923PurposeA CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA2B2 with sfgfp::ASV).DepositorInsertsdcas9
tetR
pspFΔHTH::λN22plus
sfgfp
UseSynthetic BiologyTagsASV tag and pspFΔHTH::λN22plusExpressionBacterialMutationMutations D10A and H840A on WT cas9. Synonymous m…PromoterAnderson promoter: J23106, PpspA-LEA2B2, and PtetAvailable SinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTH734-2µ-RLuc/stopCFLuc
Plasmid#40609DepositorInsertsFirefly Luciferase (nonsense mutant)
Renilla luciferase
ExpressionYeastMutationAll codons of the original FLuc gene were exchang…PromoterADH1 and TDH3Available SinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTH753-CEN-RLuc/min346maxCFLuc
Plasmid#38219DepositorInsertsFirefly Luciferase
Renilla luciferase
ExpressionYeastMutationThe first 346 codons of the original FLuc gene we…PromoterADH1 and TDH3 (=GPD)Available SinceOct. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTH752-CEN-RLuc/min103maxCFLuc
Plasmid#38218DepositorInsertsFirefly Luciferase
Renilla luciferase
ExpressionYeastMutationThe first 103 codons of the original FLuc gene we…PromoterADH1 and TDH3 (=GPD)Available SinceOct. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTH751-CEN-RLuc/min53maxCFLuc
Plasmid#38217DepositorInsertsFirefly Luciferase
Renilla luciferase
ExpressionYeastMutationThe first 53 codons of the original FLuc gene wer…PromoterADH1 and TDH3 (=GPD)Available SinceOct. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
TMD24
Plasmid#163678PurposeExpresses a ST6GAL1 mutant with 24 amino acids in its TMD in mammalian cellsDepositorInsertpartial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)
TagsGFPExpressionMammalianMutationFor this partial length ST chimera, two restricti…Available SinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDual
Plasmid#207883PurposeExpresses thermosensitive variants of the homing endonucleases I-OnuI and I-GpeMI, each in a different open reading frame. Both were made thermosensitive by a fusion with the L212P VMA1 intein.DepositorInsertsThermosensitive fusion of I-OnuI and the L212P VMA1 intein.
Thermosensitive fusion of I-GpeMI and the L212P VMA1 intein.
UseSynthetic BiologyExpressionBacterialMutationLeucine 212 changed to proline, leucine 434 in th…Available SinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAdeasy-CMV-AE-EF1α-R2
Plasmid#194928Purposeexpressing two fusion proteins of Ag85B-ESAT6 and Rv2031c-Rv2626c from multi-stage antigens of Mycobacterium tuberculosisDepositorInsertsUseAdenoviralTagsAg85B-ESAT6, EF1a, and Rv2031c-Rv2626cMutationa 48 bp linker (Gly3ser)4 was added between the t…PromoterCMV and EF1aAvailable SinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
JEC1536
Plasmid#253200PurposeReengineered pMB1 (Repressor -LS/Target-LS)DepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 6, 2026AvailabilityAcademic Institutions and Nonprofits only -
JEC1535
Plasmid#253199PurposeReengineered pMB1 (Repressor -WT/Target-LS)DepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 6, 2026AvailabilityAcademic Institutions and Nonprofits only -
JEC1533
Plasmid#253197PurposeReengineered pMB1 (Repressor -WT/Target-WT)DepositorTypeEmpty backboneExpressionBacterialAvailable SinceMarch 30, 2026AvailabilityAcademic Institutions and Nonprofits only -
JEC1534
Plasmid#253198PurposeReengineered pMB1 (Repressor -LS/Target-WT)DepositorTypeEmpty backboneExpressionBacterialAvailable SinceMarch 30, 2026AvailabilityAcademic Institutions and Nonprofits only