We narrowed to 32,148 results for: eng

1. pCS-PesaR-Dlux

   Plasmid

   #47653

   Purpose

   PesaR-D promoter in pCS26

   Depositor

   Cynthia Collins

   Article

   Shong et al ACS Synth Biol. 2013 Jul 23.

   Insert

   PesaR-D
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   Added an additional esa box upstream of -35 site …

   Promoter

   PesaR-D

   Available Since

   Oct. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pEXP5-NT-AlleyCat

   Plasmid

   #41715

   Depositor

   William DeGrado, Ivan Korendovych

   Article

   Korendovych et al Proc Natl Acad Sci U S A. 2011 Apr 26;10

   Insert

   mutated C-terminal portion of calmodulin gene (M76-K148)
   

   Tags

   Polyhistidine (6×His) region, TEV recognition sit…

   Expression

   Bacterial

   Mutation

   F92E

   Promoter

   T7

   Available Since

   May 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFredB-PTTG1

   Plasmid

   #16585

   Depositor

   Bert Vogelstein

   Article

   Jallepalli et al Cell. 2001 May 18. 105(4):445-57.

   Insert

   PTTG1 (PTTG1 Human)
   

   Expression

   Mammalian

   Mutation

   3' targeting element

   Available Since

   April 7, 2008

   Availability

   Academic Institutions and Nonprofits only

4. pMT Ptp69D-GFP

   Plasmid

   #126663

   Purpose

   Inducible expression of Ptp69D-GFP in S2 cells

   Depositor

   Ron Vale

   Article

   Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

   Insert

   Ptp69D
   

   Expression

   Insect

   Mutation

   A53S, K105N, and A98T- please see depositor comme…

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET His10-Draper INT

   Plasmid

   #126670

   Purpose

   Expression of His10-Draper intracellular domain for purification from E. coli

   Depositor

   Ron Vale

   Article

   Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

   Insert

   Draper cytoplasmic domain
   

   Expression

   Bacterial

   Availability

   Academic Institutions and Nonprofits only

   

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6. C119

   Plasmid

   #174354

   Purpose

   CRISPR-Cas9 with guide targeting human PABPC1 genomic location near STOP codon

   Depositor

   David Bartel

   Article

   Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

   Insert

   gRNA sequence targeting human PABPC1 near stop codon region
   

   Use

   CRISPR

   Availability

   Academic Institutions and Nonprofits only

   

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7. C205

   Plasmid

   #174359

   Purpose

   For expressing siRNA-resistant frog ePAB in human cell lines via transfection

   Depositor

   David Bartel

   Article

   Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

   Insert

   ePAB
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Multiple silent mutations introduced to be resist…

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR_EGFPligand

   Plasmid

   #79129

   Purpose

   Lentiviral vector for surface expressed EGFP (SFFV promoter)

   Depositor

   Wendell Lim

   Article

   Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

   Insert

   EGFP surface ligand
   

   Use

   Lentiviral

   Available Since

   July 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. TRE-mCherry-p2A-eNpHR3.0-TS

   Plasmid

   #158703

   Purpose

   Reporter construct

   Depositor

   Alice Ting

   Article

   Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

   Insert

   mCherry-p2A-eNpHR3.0-TS
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDisplay-mSA-EGFP-TM

    Plasmid

    #39863

    Depositor

    Sheldon Park

    Article

    Lim et al Biotechnol Bioeng. 2013 Jan;110(1):57-67

    Insert

    monomeric streptavidin, EGFP, TM helix
    

    Tags

    EGFP, FLAG, and HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. p426-SNR52p-gRNA.CAN1.Y-SUP4t

    Plasmid

    #43803

    Purpose

    Encodes a gRNA that targets Can1.Y in yeast.

    Depositor

    George Church

    Article

    Dicarlo et al Nucleic Acids Res

    Insert

    CAN1.y gRNA (CAN1 Budding Yeast)
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    SNR52

    Available Since

    March 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pimEJ5GFP

    Plasmid

    #44026

    Depositor

    Jeremy Stark

    Article

    Bennardo et al PLoS Genet. 2008 Jun 27;4(6):e1000110. d

    Insert

    EJ5GFP egfp-based chromosomal break reporter
    

    Expression

    Mammalian

    Mutation

    pCAGGS promoter and eGFP separated by pgkPURO cas…

    Promoter

    pCAGGS

    Available Since

    May 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHR_Gal4UAS_tBFP_PGK_mCherry

    Plasmid

    #79130

    Purpose

    Lentiviral vector - tBFP reporter for Gal4DBDVP64 synNotch receptors with a constitutive mCherry

    Depositor

    Wendell Lim

    Article

    Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

    Insert

    tBFP
    

    Use

    Lentiviral

    Available Since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pND003

    Plasmid

    #200949

    Purpose

    Yeast surface display destination vector for Golden Gate cloning

    Depositor

    Timothy Whitehead

    Article

    Daffern et al Biotechnol Bioeng. 2024 Jan;121(1):281-2

    Type

    Empty backbone

    Use

    Yeast surface display

    Tags

    AGA2, HA, and Myc

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    June 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. phU6-gRNA

    Plasmid

    #53188

    Purpose

    Expresses the S. pyogenes sgRNA from the human U6 promoter

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    human U6

    Available Since

    June 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDEST-CMV-N-mCherry

    Plasmid

    #123215

    Purpose

    N-terminal mCherry tag. Gateway destination vector for mammalian expression.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDEST-CMV-C-EGFP

    Plasmid

    #122844

    Purpose

    C-terminal EGFP tag. Gateway destination vector for mammalian expression.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDEST-CMV-N-EGFP

    Plasmid

    #122842

    Purpose

    N-terminal EGFP tag. Gateway destination vector for mammalian expression.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKM496 (deletions) Zeo

    Plasmid

    #109301

    Purpose

    ORBIT integrating plasmid for deletion of target gene, using zeocin as a selection marker.

    Depositor

    Kenan Murphy

    Article

    Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

    Insert

    Bxb1 attB site
    

    Use

    Orbit integrating plasmid

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pACYC-GroEL/ES-TF

    Plasmid

    #83923

    Purpose

    Co-expresses cytoplasmic copies of the Gro EL/ES and Trigger Factor protein folding chaperones.

    Depositor

    Karl Griswold

    Article

    Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

    Inserts

    Gro EL/ES
    Trigger Factor
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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