We narrowed to 9,399 results for: BLI

1. pLV-SFFV-LNPK-WPRE-UbC-mCherry

   Plasmid

   #225954

   Purpose

   Lentiviral vector plasmid expressing human lunapark (LNPK) under the spleen focus-forming virus (SFFV) promoter and mCherry under the Ubiquitin C (UbC) promoter

   Depositor

   Richard Eva, Bart Nieuwenhuis, Evan Reid

   Inserts

   LNPK (LNPK Human)
   mCherry
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   SFFV and UbC

   Available Since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-rTetR(SE)-DNMT3Bcd-2A-mTurquoise

   Plasmid

   #225693

   Purpose

   Lentiviral expression of rTetR(SE) fused to the catalytic domain of DNMT3B and mTurquoise-NLS

   Depositor

   Lacra Bintu

   Article

   Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

   Insert

   rTetR-DNMT3B catalytic domain (DNMT3B Synthetic, Human)
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   3xFLAG and T2A-mTurquoise

   Expression

   Mammalian

   Available Since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pWR63(YUM1)

   Plasmid

   #203336

   Purpose

   episomal Cas9 and YUM1-targeting sgRNA expression vector for S. stipitis

   Depositor

   J. Brian Robertson

   Article

   Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

   Inserts

   TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
   coHPH
   ACT1 terminator (S. stipitis)
   CCW12 promoter (S. stipitis)
   CaCas9
   ADH2 terminator (S. stipitis)
   ARS from S. stipitis chromosome 1
   THD3 promoter (S. stipitis)
   tRNA-sgRNA(SapI)-HDV
   ADH1 terminator (S. stipitis)
   YUM1-targeting sequence
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pWR63

   Plasmid

   #203331

   Purpose

   episomal Cas9 and sgRNA expression vector without sgRNA for S. stipitis

   Depositor

   J. Brian Robertson

   Article

   Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

   Inserts

   TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
   coHPH
   ACT1 terminator (S. stipitis)
   CCW12 promoter (S. stipitis)
   CaCas9
   ADH2 terminator (S. stipitis)
   ARS from S. stipitis chromosome 1
   THD3 promoter (S. stipitis)
   tRNA-sgRNA(SapI)-HDV
   ADH1 terminator (S. stipitis)
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENTR-Creb5(T59/T61A)(HA)

   Plasmid

   #195025

   Purpose

   Gateway vector containing HA-tagged Creb5 with T59/T61A mutation

   Depositor

   Andrew Lassar, Cheng-Hai Zhang

   Article

   Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

   Insert

   HA-tagged Creb5 (full length, 508aa; with with T59/T61A mutation) (CREB5 Bovine)
   

   Use

   Gateway cloning

   Tags

   3xHA

   Mutation

   T59/T61A

   Available Since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR-Creb5(T59/T61D)(HA)

   Plasmid

   #195026

   Purpose

   Gateway vector containing HA-tagged Creb5 with T59/T61D mutation

   Depositor

   Andrew Lassar, Cheng-Hai Zhang

   Article

   Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

   Insert

   HA-tagged Creb5 (full length, 508aa; with with T59/T61D mutation) (CREB5 Bovine)
   

   Use

   Gateway cloning

   Tags

   3xHA

   Mutation

   T59/T61D

   Available Since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-Creb5(HA)

   Plasmid

   #195024

   Purpose

   HA-tagged Creb5 in pLenti CMV Puro DEST (w118-1) vector

   Depositor

   Andrew Lassar, Cheng-Hai Zhang

   Article

   Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

   Insert

   HA-tagged Creb5 (full length, 508aa) (CREB5 Bovine)
   

   Use

   Lentiviral

   Tags

   3xHA

   Expression

   Mammalian

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pInducer20-iCreb5(T59/T61A)(HA)

   Plasmid

   #195027

   Purpose

   HA-tagged Creb5(T59/T61A) in pInducer20 lentivirus destination vector

   Depositor

   Andrew Lassar, Cheng-Hai Zhang

   Article

   Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

   Insert

   HA-tagged Creb5 (full length, 508aa; with T59/T61A mutation) (CREB5 Bovine)
   

   Use

   Lentiviral

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   T59/T61A

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pInducer20-iCreb5(T59/T61D)(HA)

   Plasmid

   #195028

   Purpose

   HA-tagged Creb5(T59/T61D) in pInducer20 lentivirus destination vector

   Depositor

   Andrew Lassar, Cheng-Hai Zhang

   Article

   Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

   Insert

   HA-tagged Creb5 (full length, 508aa; with with T59/T61D mutation) (CREB5 Bovine)
   

   Use

   Lentiviral

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   T59/T61D

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. An HA CAAX pMT2

    Plasmid

    #206116

    Purpose

    expression of Cav2.1 N-terminus (amino acids 1-100) with a C-terminal CAAX (last 10aa of H.Ras) motif and a HA tag

    Depositor

    Annette Dolphin

    Article

    Page et al J Biol Chem. 2010 Jan 8;285(2):835-44. d

    Insert

    cacna1a (Cacna1a Rat)
    

    Expression

    Mammalian

    Promoter

    Ad MLP/TPL/SV40

    Available Since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. An CAAX pMT2

    Plasmid

    #206118

    Purpose

    expression of Cav2.1 N-terminus (amino acids 1-100) with C-terminal CAAX (last 10 aa of H.Ras) motif

    Depositor

    Annette Dolphin

    Article

    Page et al J Biol Chem. 2010 Jan 8;285(2):835-44. d

    Insert

    cacna1a (Cacna1a Rat)
    

    Expression

    Mammalian

    Promoter

    Ad MLP/TPL/SV40

    Available Since

    Oct. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. GFP Cav2.2 D122A pcDNA3

    Plasmid

    #206068

    Purpose

    expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and a D122A mutation that disrupts the interaction with ?2?-1

    Depositor

    Annette Dolphin

    Article

    Page et al J Neurosci. 2004 Jun 9;24(23):5400-9. do

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGL3-pSMANTIS_WT_1kb

    Plasmid

    #194187

    Purpose

    Includes the promoter (1kb) of SMTS (SMANTIS, MANTIS, AK125871)

    Depositor

    Ralf Brandes, Matthias Leisegang

    Article

    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

    Insert

    SMANTIS promoter (1kb) (SMANTIS Human)
    

    Use

    Luciferase

    Promoter

    SMANTIS promoter (1kb)

    Available Since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. deltaVP1 + VP2C-MIOX

    Plasmid

    #166680

    Purpose

    Yeast integrative plasmid for expressing deltaVP1 (GAL1 promoter) and mouse myo-inositol oxygenase tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

    Inserts

    VP2C-MIOX
    Murine polyomavirus deltaVP1 (NLS deletion mutant)
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Promoter

    GAL1 and GAL10

    Available Since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. wtVP1 + VP2C-GFP

    Plasmid

    #166674

    Purpose

    Yeast integrative plasmid for expressing wild-type Murine polyomavirus VP1 (GAL1 promoter) and GFP tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

    Inserts

    VP2C-GFP
    Wild-type Murine polyomavirus VP1
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Promoter

    GAL1 and GAL10

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. p1A Prk-

    Plasmid

    #162694

    Purpose

    p1A negative control expressing CbbLS and inactive Prk (Prk K20M S21A)

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    H. neapolitanus CbbLS and S. elongatus Prk
    

    Tags

    N terminal 6x His tag on PRK

    Expression

    Bacterial

    Mutation

    Prk inactive (K20M S21A native protein, this map …

    Promoter

    PLtet0-1 promoter

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. FMR1-E17B-P2A-NLuc

    Plasmid

    #157857

    Purpose

    donor plasmid for FMR1-Nluc

    Depositor

    Xinyu Zhao

    Article

    Li et al Stem Cells. 2017 Jan;35(1):158-169. doi:

    Insert

    FMR1-Nlu (FMR1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAVS1 intron 1 gRNA s3

    Plasmid

    #132395

    Purpose

    Targets AAVS1 intron 1, gRNA: GGGGCCACTAGGGACAGGAT, MLM3636 backbone

    Depositor

    Gerold Schmitt-Ulms

    Article

    Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

    Insert

    gRNA targeting AAVS1 intron 1 (PPP1R12C Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJC20cdc8.27

    Plasmid

    #99362

    Purpose

    Bacterial expression vector containing cDNA encoding for the temperature sensitive fission yeast tropomyosin mutant, Cdc8.27.

    Depositor

    Dan Mulvihill

    Article

    Johnson et al Biochem Biophys Res Commun. 2017 Oct 25.

    Insert

    cdc8 (cdc8 Fission Yeast)
    

    Expression

    Bacterial

    Mutation

    Glutamic acid 129 to Lysine

    Promoter

    T7

    Available Since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. RGS-6xHis-LIN-29-pcDNA3.1-

    Plasmid

    #52515

    Purpose

    expresses C. elegans LIN-29 in mammalian cells with RGS-6xHis tag at N-terminus

    Depositor

    Adam Antebi

    Article

    Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

    Insert

    Lin-29 b (lin-29 Nematode)
    

    Tags

    RGS-6xHis

    Expression

    Mammalian

    Mutation

    wt (changed coding sequence in 5' (5th codon…

    Promoter

    CMV

    Available Since

    Aug. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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