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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 13,677 results for: FLU;

Showing: 8541 - 8560 of 13677 results

  5. LSB-hsa-let-7f-1-3p

    Plasmid
    #103154
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-let-7f-1-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-let-7f-1-3p target (MIRLET7F1 Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103154-plasmid-map-sequence-id-213240

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  7. pIA33

    Plasmid
    #120803
    Purpose
    E. coli-C. difficile shuttle vector for CRISPR interference (CRISPRi) in C. difficile; sgRNA targets rfp; Pxyl::dCas9-opt Pgdh::sgRNA-rfp
    Depositor
    Craig Ellermeier, David S. Weiss
    Article
    Muh et al J Bacteriol. 2019 Feb 11. pii: JB.00711-
    Inserts
    deactivated nuclease dCas9, codon-optimized for C. difficile
    single guide RNA targeting red fluorescent protein
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    Base-pairing region targets red fluorescent prote…
    Promoter
    Pgdh and Pxyl
    Available Since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    120803-plasmid-map-sequence-id-235316

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  15. GABA(A) receptor subunit g2SE

    Plasmid
    #49170
    Purpose
    pHluorin-tagged GABA A receptor subunit (gamma 2) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surface
    Depositor
    Tija Jacob, Stephen Moss
    Article
    Jacob et al J Neurosci. 2005 Nov 9;25(45):10469-78.
    Insert
    GABA(A) receptor subunit gamma 2 (Gabrg2 Mouse)
    Tags
    myc, pHGFP (pHluorin), and thrombin cleavage site…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 10, 2013
    Availability
    Academic Institutions and Nonprofits only
    49170-plasmid-map-sequence-id-165320

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  16. pETM6-G6-vioABE

    Plasmid
    #73438
    Purpose
    Prodeoxyviolacein pathway (vioABE) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variant G6.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    PG6-vioABE
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    PG6 (orthogonal T7-lac variant)
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73438-plasmid-map-sequence-id-135337

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  18. pXPR_071

    Plasmid
    #164558
    Purpose
    Higher titer version of pXPR_053. Lentiviral vector that enables constitutive sgRNA expression. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.
    Depositor
    Arlene Sharpe
    Article
    LaFleur et al Nat Immunol. 2023 Nov 27. doi: 10.1038/s
    Insert
    sgRNA cassette
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    Human U6
    Available Since
    Nov. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    164558-plasmid-map-sequence-id-330194

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Showing: 8541 - 8560 of 13677 results