We narrowed to 2,360 results for: Efs

1. pPKm-235

   Plasmid

   #90509

   Purpose

   pSIN - EF-1alpha - MTS sHO1 - P2A - MTS - Hy2, vector encoding for mitochondrial-tagged Synechococcus sp. Heme Oxigenase-1 (HO1) and phytochromobilin:ferredoxin oxidoreductase (Hy2)

   Depositor

   Todd Coleman

   Article

   Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

   Insert

   mito-sHO1, mito-Hy2
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPKm-233

   Plasmid

   #90508

   Purpose

   pSIN - EF-1alpha - sFD - P2A - MTS - sFNR, vector encoding for Synechococcus sp. Ferredoxin (Fd) and Ferredoxin-oxireductase (FNR)

   Depositor

   Todd Coleman

   Article

   Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

   Insert

   mito-sFD, mito-sFNR
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pPB9v2

   Plasmid

   #210029

   Purpose

   Contains cloning site for molecularly-tagged Cas9 gRNA constructs, inducible Cas9, rtTA-T2A-puro

   Depositor

   Michael Talkowski

   Article

   Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

   Inserts

   Cas9
   rtta-T2A-puro
   

   Use

   CRISPR

   Tags

   SV40 NLS and nucleoplasmin NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1α and TRE3G

   Available since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPB758

   Plasmid

   #210033

   Purpose

   Contains inducible Cas7-P2A-Cas5-T2A-Cas8, rtTA-T2A-puro

   Depositor

   Michael Talkowski

   Article

   Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

   Inserts

   Cas7-P2A-Cas5-T2A-Cas8
   rtta-T2A-puro
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1α and TRE3G

   Available since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pPB9

   Plasmid

   #210036

   Purpose

   Contains Cas9 gRNA cloning site, inducible Cas9, UCOE, puro-T2A-eGFP

   Depositor

   Michael Talkowski

   Article

   Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

   Inserts

   Cas9
   puro-T2A-eGFP
   

   Use

   CRISPR

   Tags

   SV40 NLS and nucleoplasmin NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1α and TRE3G

   Available since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLentiCRISPRpuro-sgROSA26

   Plasmid

   #231562

   Purpose

   pLentiCRISPR expression vector for Cas9 and gRNA targeting human ROSA26 locus (Puromycin selection marker)

   Depositor

   Rizwan Haq

   Article

   Schiantarelli et al Cancer Cell. 2025 Feb 10;43(2):308-316.e

   Insert

   sgROSA26
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, EF-1a

   Available since

   Feb. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. LVDP 2P.2.EGFP

   Plasmid

   #231905

   Purpose

   For the production of SARS-CoV-2 virus-like particles (VLPs) in the '2-plasmid' system and packaging EGFP mRNA into the VLPs

   Depositor

   Sriram Neelamegham

   Article

   Yang et al Npj Viruses. 2025 Jan 25;3(1):4. doi: 10

   Insert

   nucleocapsid protein-T2A-membrane protein-IRES-envelope protein (N, E, M SARS-CoV-2)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   R203M in N protein

   Promoter

   CMV, EF-1a

   Available since

   Jan. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. FLAG NC

   Plasmid

   #214675

   Purpose

   Negative control plasmid for splicing reporters consisting of MCP fused FLAG epitope tags

   Depositor

   Eugene Yeo

   Article

   Schmok et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

   Insert

   FLAG
   

   Use

   Tags

   MCP and V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   March 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. MCAM-BirA*-HA

   Plasmid

   #214472

   Purpose

   Lentiviral vector for the expression of MCAM-BirA*-HA fusion protein in mammalian cells.

   Depositor

   Natalie Ahn

   Article

   Miller et al Mol Biol Cell. 2023 Dec 20:mbcE23060255.

   Insert

   MCAM (MCAM Human)
   

   Use

   Lentiviral; Gateway

   Tags

   BirA(R118G) and HA tag

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   March 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLenti-Cas9-ZNF746

    Plasmid

    #207822

    Purpose

    Dual expression of Cas9 and sgRNA targeting ZNF746

    Depositor

    Ben Major

    Article

    LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

    Insert

    ZNF746 sgRNA (ZNF746 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    EF-1a; U6

    Available since

    Dec. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pN1mcherry-NKD2EFMUT

    Plasmid

    #174333

    Purpose

    encodes human NKD2 132DFD134>AFA mutant (two D mutated to A)cDNA with a C-terminal mCherry fusion.

    Depositor

    Sonal Srikanth

    Article

    Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

    Insert

    Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
    

    Use

    Tags

    mCherry tag

    Expression

    Mammalian

    Mutation

    NKD2132DFD134>AFA (substitutions of D132 and D…

    Promoter

    CMV

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. SaLgCG

    Plasmid

    #164563

    Purpose

    GFP

    Depositor

    Junwei Shi

    Article

    Verma et al Nat Cell Biol. 2021 Jan 18. pii: 10.1038

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter for crRNA expression and EFS promoter…

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAWp63-clone3-12 (OptiHF-SpCas9)

    Plasmid

    #131737

    Purpose

    Expresses OptiHF-SpCas9 in mammalian cells

    Depositor

    Alan Siu Lun Wong

    Article

    Choi et al Nat Methods. 2019 Aug;16(8):722-730. doi

    Insert

    OptiHF-SpCas9
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mutation

    Q695A+K848A+E923M+T924V+Q926A

    Promoter

    EFS

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. LSB-hsa-miR-99b-3p

    Plasmid

    #103766

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-99b-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-99b-3p target (MIR99B Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. LSB-hsa-miR-9-3p

    Plasmid

    #103748

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-9-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-9-3p target
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LSB-hsa-miR-92a-3p

    Plasmid

    #103752

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-92a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-92a-3p target
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. LSB-hsa-miR-765

    Plasmid

    #103730

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-765 target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-765 target (MIR765 Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. LSB-hsa-miR-802

    Plasmid

    #103737

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-802 target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-802 target (MIR802 Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LSB-hsa-miR-657

    Plasmid

    #103712

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-657 target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-657 target (MIR657 Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LSB-hsa-miR-634

    Plasmid

    #103692

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-634 target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-634 target (MIR634 Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. LSB-hsa-miR-635

    Plasmid

    #103693

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-635 target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-635 target (MIR635 Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. LSB-hsa-miR-640

    Plasmid

    #103695

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-640 target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-640 target (MIR640 Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. LSB-hsa-miR-643

    Plasmid

    #103698

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-643 target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-643 target (MIR643 Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. LSB-hsa-miR-645

    Plasmid

    #103699

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-645 target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-645 target (MIR645 Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. LSB-hsa-miR-648

    Plasmid

    #103700

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-648 target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-648 target (MIR648 Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. LSB-hsa-miR-6503-3p

    Plasmid

    #103701

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-6503-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-6503-3p target (MIR6503 Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. LSB-hsa-miR-6503-5p

    Plasmid

    #103702

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-6503-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-6503-5p target (MIR6503 Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. LSB-hsa-miR-617

    Plasmid

    #103678

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-617 target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-617 target (MIR617 Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. LSB-hsa-miR-577

    Plasmid

    #103659

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-577 target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-577 target (MIR577 Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. LSB-hsa-miR-595

    Plasmid

    #103670

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-595 target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-595 target (MIR595 Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge