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pKG2436_pGEEC532_pShip-EF1a-mRuby2-P2A-PuroR-bGH

Plasmid

#239728

Purpose

Plasmid expressing mRuby2 and PuroR with the EF1a promoter

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2-P2A-PuroR

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pKG2437_pGEEC533_pShip-EFS-mRuby2-P2A-PuroR-bGH

Plasmid

#239729

Purpose

Plasmid expressing mRuby2 and PuroR with the EFS promoter

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2-P2A-PuroR

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pKG2438_pGEEC534_pShip-CMV-mRuby2-P2A-PuroR-bGH

Plasmid

#239730

Purpose

Plasmid expressing mRuby2 and PuroR with the CMV promoter

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2-P2A-PuroR

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pKG2439_pGEEC535_pShip-hPGK-mRuby2-P2A-PuroR-bGH

Plasmid

#239731

Purpose

Plasmid expressing mRuby2 and PuroR with the hPGK promoter

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2-P2A-PuroR

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pKG2441_pGEEC537_pShip-CAG-mRuby2-P2A-PuroR-bGH

Plasmid

#239733

Purpose

Plasmid expressing mRuby2 and PuroR with the CAG promoter

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2-P2A-PuroR

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pKG3660_pGEEC570_pShip-ZF10.BSx8-ybTATA-spacer-mRuby2-bGH

Plasmid

#239739

Purpose

Plasmid expressing mRuby2 from a SynZiFTR promoter, Induced with pKG2309

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pKG2387_pPV2-mRuby2-P2A-PuroR

Plasmid

#239696

Purpose

BsaI Golden Gate assembly plasmid encoding the mRuby2-P2A-PuroR CDS

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2-P2A-PuroR

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pRSET-Cm-CspB

Plasmid

#215400

Purpose

Cm NCPPB382 cspB codon-optimized for E. coli recombinant protein expression (6xHis-CspB)

Depositor

Gitta Coaker

Article

Stevens et al Proc Natl Acad Sci U S A. 2024 Jun 4;121

Insert

cspB

Tags

6xHis Tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pRSET-Cm-CspA2

Plasmid

#215399

Purpose

Cm NCPPB382 cspA2 codon-optimized for E. coli recombinant protein expression (6xHis-CspA2)

Depositor

Gitta Coaker

Article

Stevens et al Proc Natl Acad Sci U S A. 2024 Jun 4;121

Insert

cspA2

Tags

6xHis Tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pDSK519-cspB

Plasmid

#207162

Purpose

Expresses codon-optimized cspB from Clavibacter michiganensis in a broad host range vector

Depositor

Gitta Coaker

Article

Stevens et al Proc Natl Acad Sci U S A. 2024 Jun 4;121

Insert

cspB

Tags

HA

Expression

Bacterial

Promoter

nptII

Available Since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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1BR9-BFP

Plasmid

#202054

Purpose

E. coli expression vector for N-ter GFP11 and C-ter R9 tagged tagBFP.

Depositor

Markita Landry

Article

Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

Insert

BFP

Tags

6xHis, GFP11, and R9

Expression

Bacterial

Promoter

T7 Promoter

Available Since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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1BR9-BFPSTOP

Plasmid

#202055

Purpose

E. coli expression vector for N-ter GFP11 R9 tagged BFP

Depositor

Markita Landry

Article

Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

Insert

BFP

Tags

6xHis and GFP11

Expression

Bacterial

Promoter

T7 Promoter

Available Since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pL0_DE-RFP1

Plasmid

#202154

Purpose

Level 0 plasmid containing a RFP1 fluorescent protein with DE overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

RFP1 gene

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pL0_DE-mRuby3

Plasmid

#202153

Purpose

Level 0 plasmid containing a mRuby3 fluorescent protein with DE overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

mRuby3 gene

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_CD-mRFP1

Plasmid

#202145

Purpose

Level 0 plasmid containing a mRFP1 fluorescent protein with CD overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

mRFP1 gene

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pBMN BFP

Plasmid

#188644

Purpose

This is the Lazarou lab's standard retrovirus vector backbone for expression of BFP-tag fusions onto the N-terminus of cloned genes

Depositor

Michael Lazarou

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Type

Empty backbone

Use

Retroviral

Tags

BFP

Expression

Mammalian

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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ptRNAfMet-A37U

Plasmid

#196659

Purpose

Plasmid for overexpression of mutated initiator tRNA (A37U)

Depositor

Markus Jeschek

Article

Hollerer et al Nucleic Acids Res. 2023 Mar 21;51(5):237

Insert

tRNAfMet-A37U expression cassette

Expression

Bacterial

Mutation

A37U in tRNAfMet

Promoter

metY native promoter

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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ptRNAfMet-A37G

Plasmid

#196658

Purpose

Plasmid for overexpression of mutated initiator tRNA (A37G)

Depositor

Markus Jeschek

Article

Hollerer et al Nucleic Acids Res. 2023 Mar 21;51(5):237

Insert

tRNAfMet-A37G expression cassette

Expression

Bacterial

Mutation

A37G in tRNAfMet

Promoter

metY native promoter

Available Since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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pIIS18-BufAI-2

Plasmid

#128651

Purpose

Entry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymes

Depositor

Tohru Suzuki

Article

Altaib et al Microbiol Resour Announc. 2019 Oct 10;8(

Type

Empty backbone

Use

Unspecified

Available Since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pIIS18-BufAI-1

Plasmid

#128650

Purpose

Entry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymes

Depositor

Tohru Suzuki

Article

Altaib et al Microbiol Resour Announc. 2019 Oct 10;8(

Type

Empty backbone

Use

Unspecified

Available Since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge