We narrowed to 9,586 results for: Coli
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Plasmid#53732PurposePlpp/PlacUV5- directed synthesis of the alpha NTD fused to E. coli σ70 region 4. Used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734.DepositorInsertRNAP alpha NTD (aa 1-248) fused to E. coli sigma 70 (aa 528-613)
ExpressionBacterialMutationThe sigma 70 moiety carries the D581G substitutionPromoterlpp/lacUV5 (tandem promoter)Available SinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-yibDp2-GFPuv
Plasmid#139083PurposeLow phosphate inducible promoter yibDp2 for GFP expressionDepositorInsertyibDp2-GFPuv
ExpressionBacterialAvailable SinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMAL-EFSep
Plasmid#34627DepositorInsertEF-Sep (tufB E. coli)
Tags6xHIS and MBPExpressionBacterialMutationHis67 to Arg , Glu216 to Asn, Asp217 to Gly, Phe2…Available SinceMarch 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSMART-GFPuv
Plasmid#65822PurposeGFPuv Expression, Low Phosphate Dependent E. coli ExpressionDepositorInsertgfpuv
ExpressionBacterialPromoterE . coli insulated waaH gene promoter, low phosp…Available SinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMJA180
Plasmid#128538PurposeExpresses E coli Bir A proteinDepositorInsertE coli BirA
ExpressionYeastPromoterAOX1Available SinceAug. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4136464
Plasmid#149534PurposeBacterial expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
Tags6xHIS;Linker_GGSG;GST;Linker_GGSG;Cleavable TEVExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJES103
Plasmid#135410PurposeE. coli 'blaTEM-1 with M. tuberculosis mpt63 signal sequence under the control of hsp60 promoter that can be used as a reporterDepositorInsertblaTEM-1
ExpressionBacterialMutationE. coli 'blaTEM-1 with M. tuberculosis mpt63…Promoterhsp60Available SinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4136461
Plasmid#149538PurposeBacterial expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
Tags6xHIS;Linker_GGSG;MBP;Linker_GGSG;Cleavable TEVExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046758
Plasmid#145670PurposeBacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;HAExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCS4
Plasmid#55751PurposeProduces one half (alpha) of aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.DepositorInsertKanR
ExpressionBacterialMutationResidues 1-59 of KanRPromoterT7-lacAvailable SinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKM263-EcPPXc
Plasmid#38328DepositorInsertE. coli polyphosphatase (ppx Escherichia coli)
Tags6xHIS, GST, and TEVExpressionBacterialMutationC-terminus of E. coli polyphosphatase (described …PromoterT7Available SinceAug. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4136463
Plasmid#149527PurposeBacterial expression plasmid for SARS-CoV-2 E (envelope)DepositorInsertSARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
Tags6xHIS;Linker_GGSG;SUMO tag;Linker_GGSG;Cleavable …ExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSA10(gfcB-His-CD)
Plasmid#176194Purposeexpresses 3 overlapping genes (gfcB-6xHis, gfcC, gfcD) from this one operonDepositorInsertgfcBCD
TagsGfcB-6xHisExpressionBacterialPromoterPtacAvailable SinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBH31(gfcD)
Plasmid#176199PurposeEncodes pelC signal sequence followed by His6-GfcD. Should secrete to periplasmDepositorInsertgfcD
TagspelC leader-6xHisExpressionBacterialPromoterT7Available SinceOct. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBH31(gfcD-10xHis)
Plasmid#176200PurposeEncodes pelC signal sequence followed by His10-GfcD. Should secrete to periplasmDepositorInsertgfcD
TagspelC leader-10xHisExpressionBacterialPromoterT7Available SinceOct. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS5
Plasmid#55750PurposeProduces one half (beta) of aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.DepositorInsertKanR
ExpressionBacterialMutationResidues 59-264 of KanRPromoterT7-lacAvailable SinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046801
Plasmid#145741PurposeBacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;MycExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4136454
Plasmid#149529PurposeBacterial expression plasmid for SARS-CoV-2 M (membrane)DepositorInsertSARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
Tags6xHIS;Linker_GGSG;SUMO tag;Linker_GGSG;Cleavable …ExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceMay 13, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046931
Plasmid#145706PurposeBacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;FLAG_2ExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4136460
Plasmid#149532PurposeBacterial expression plasmid for SARS-CoV-2 E (envelope)DepositorInsertSARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
Tags6xHIS;Linker_GGSG;MBP;Linker_GGSG;Cleavable TEVExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits