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NDC80 F12.1 gRNA

Plasmid

#90787

Purpose

3rd generation lentiviral gRNA plasmid targeting human NDC80

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

NDC80 (Guide Designation F12.1)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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NDC80 F11.1 gRNA

Plasmid

#90786

Purpose

3rd generation lentiviral gRNA plasmid targeting human NDC80

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

NDC80 (Guide Designation F11.1)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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SGO1 H1.1 gRNA

Plasmid

#90886

Purpose

3rd generation lentiviral gRNA plasmid targeting human SGO1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

SGO1 (Guide Designation H1.1)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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SGO1 H2.1 gRNA

Plasmid

#90887

Purpose

3rd generation lentiviral gRNA plasmid targeting human SGO1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

SGO1 (Guide Designation H2.1)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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ASF1B A4.2 gRNA

Plasmid

#90535

Purpose

3rd generation lentiviral gRNA plasmid targeting human ASF1B

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

ASF1B (Guide Designation A4.2)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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ASF1B A3.2 gRNA

Plasmid

#90534

Purpose

3rd generation lentiviral gRNA plasmid targeting human ASF1B

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

ASF1B (Guide Designation A3.2)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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TUBE1 F6.4 gRNA

Plasmid

#90929

Purpose

3rd generation lentiviral gRNA plasmid targeting human TUBE1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

TUBE1 (Guide Designation F6.4)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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BUB3 A10.1 gRNA

Plasmid

#90557

Purpose

3rd generation lentiviral gRNA plasmid targeting human BUB3

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

BUB3 (Guide Designation A10.1)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 17, 2017

Availability

Academic Institutions and Nonprofits only

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NDEL1 F4.2 gRNA

Plasmid

#90792

Purpose

3rd generation lentiviral gRNA plasmid targeting human NDEL1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

NDEL1 (Guide Designation F4.2)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

Aug. 15, 2017

Availability

Academic Institutions and Nonprofits only

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NDEL1 F3.2 gRNA

Plasmid

#90790

Purpose

3rd generation lentiviral gRNA plasmid targeting human NDEL1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

NDEL1 (Guide Designation F3.2)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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PPP2R5E C1.5 gRNA

Plasmid

#90855

Purpose

3rd generation lentiviral gRNA plasmid targeting human PPP2R5E

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

PPP2R5E (Guide Designation C1.5)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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TPX2 H2.2 gRNA

Plasmid

#90919

Purpose

3rd generation lentiviral gRNA plasmid targeting human TPX2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

TPX2 (Guide Designation H2.2)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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CTPS1 E12.3 gRNA

Plasmid

#90645

Purpose

3rd generation lentiviral gRNA plasmid targeting human CTPS1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CTPS1 (Guide Designation E12.3)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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CTPS1 E11.3 gRNA

Plasmid

#90644

Purpose

3rd generation lentiviral gRNA plasmid targeting human CTPS1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CTPS1 (Guide Designation E11.3)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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FBXO5 D4.4 gRNA

Plasmid

#90687

Purpose

3rd generation lentiviral gRNA plasmid targeting human FBXO5

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

FBXO5 (Guide Designation D4.4)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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MAD2L1BP F1.1 gRNA

Plasmid

#90744

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAD2L1BP

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

MAD2L1BP (Guide Designation F1.1)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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MAD2L1BP F2.1 gRNA

Plasmid

#90745

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAD2L1BP

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

MAD2L1BP (Guide Designation F2.1)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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pEF-HA/Net1 Δ1-121

Plasmid

#28263

Depositor

Jeffrey Frost

Article

Carr et al J Biol Chem. 2009 Sep 4. 284(36):24269-8

Insert

Net1 (Net1 Mouse)

Use

Tags

2x HA

Expression

Mammalian

Mutation

Missing aa 1-121

Promoter

Available since

April 11, 2011

Availability

Academic Institutions and Nonprofits only
pYPQAT58

Plasmid

#223430

Purpose

T-DNA vector for dSpCas9 mediated gene activation for monocot plants; NGG PAM; dSpCas9-Act3.0 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; Hygromycin for plants selection.

Depositor

Yiping Qi

Article

Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

Insert

ZmUbi-dSpCas9-Act3.0-OsU3-gRNA scaffold 2.0

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Plant

Mutation

Promoter

Available since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pYPQAT61

Plasmid

#223433

Purpose

T-DNA vector for dSpCas9 mediated gene activation for monocot plants; NGG PAM; dSpCas9-Act3.0 was driven by ZmUbi1 and the sgRNA was driven by ZmUbi1 promoter; Hygromycin for plants selection.

Depositor

Yiping Qi

Article

Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

Insert

ZmUbi-dSpCas9-Act3.0-ZmUbi-gRNA scaffold 2.0-tRNA

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Plant

Mutation

Promoter

Available since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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