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We narrowed to 15,735 results for: LEA

Showing: 8581 - 8600 of 15735 results

  1. NKAP.001.140

    Plasmid
    #137206
    Purpose
    Expresses N-terminal (His)6-TEV cleavage site fusion of human gene or portion of gene in bacterial strains. pET based vector.
    Depositor
    Tara Davis
    Insert
    NKAP (NKAP Human)
    Tags
    (His)6-TEV
    Expression
    Bacterial
    Mutation
    001-140
    Available Since
    Jan. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    137206-plasmid-map-sequence-id-322594

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  2. UC16m

    Plasmid
    #121038
    Purpose
    MoClo golden gate assembly DE part for gQi gRNA (guide RNA for S. pyogenes Cas9; sequence from DOI: 10.1016/j.cell.2013.02.022). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    gQi Cas9 gRNA UC part
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121038-plasmid-map-sequence-id-236383

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  3. UC17m

    Plasmid
    #121039
    Purpose
    MoClo golden gate assembly DE part for gV1 gRNA (guide RNA for S. pyogenes Cas9; sequence from doi: [10.15252/msb.20145735]). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    gV1 Cas9 gRNA UC part
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121039-plasmid-map-sequence-id-236352

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  4. C105m

    Plasmid
    #121008
    Purpose
    MoClo golden gate assembly CD part for sfYFP-AAV (superfolder YFP with AAV degradation tag for ssrA). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    sfYFP-AAV ssrA (has bbsI)
    Use
    Synthetic Biology
    Tags
    AAV degradation tag for ssrA
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121008-plasmid-map-sequence-id-236163

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  5. C106m

    Plasmid
    #121009
    Purpose
    MoClo golden gate assembly CD part for sfYFP-DAS (superfolder YFP with DAS degradation tag for ssrA). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    sfYFP-DAS ssrA (has bbsI)
    Use
    Synthetic Biology
    Tags
    DAS degradation tag for ssrA
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121009-plasmid-map-sequence-id-236172

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  6. C109m

    Plasmid
    #121011
    Purpose
    MoClo golden gate assembly CD part for mfLon-pdt3 (mfLon protease with self-targeting degradation tag pdt3). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    mfLon protease with pdt3 tag
    Use
    Synthetic Biology
    Tags
    pdt3 mfLon degradation tag
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121011-plasmid-map-sequence-id-236323

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  8. DsRed-CPI-17-T38A-NLS

    Plasmid
    #100792
    Purpose
    Mammalian expression of Fluorescent CPI-17 neutral mutation nuclear
    Depositor
    Joseph Gordon
    Insert
    protein phosphatase 1 regulatory inhibitor subunit 14A (PPP1R14A Human)
    Tags
    DsRed
    Expression
    Mammalian
    Mutation
    T38A
    Promoter
    CMV
    Available Since
    Nov. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    100792-plasmid-map-sequence-id-193236

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  9. Py-U5-15kD

    Plasmid
    #25561
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    Py-U5-15kD
    Tags
    His
    Expression
    Bacterial
    Available Since
    May 7, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits

  10. pET His6 Sumo TEV LIC cloning vector (1S)

    Plasmid
    #29659
    Depositor
    Scott Gradia
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Tags
    6xHis, SUMO, and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available Since
    June 7, 2011
    Availability
    Academic Institutions and Nonprofits only
    29659-plasmid-map-sequence-id-354447

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  11. pET His6 MBP TEV LIC cloning vector (1M)

    Plasmid
    #29656
    Depositor
    Scott Gradia
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Tags
    6xHis, MBP, and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available Since
    Aug. 17, 2011
    Availability
    Academic Institutions and Nonprofits only
    29656-plasmid-map-sequence-id-237947

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  12. pUBC-SV40NLS-Pfv-Sapphire

    Plasmid
    #203653
    Purpose
    Mammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC promoter.
    Depositor
    Liam Holt
    Article
    Shu T et et al bioRxiv 2022
    Insert
    PfV
    Use
    Lentiviral
    Tags
    Sapphire
    Available Since
    July 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    203653-plasmid-map-sequence-id-402484

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  13. pOpen-phi29pol

    Plasmid
    #165573
    Purpose
    DNA polymerase responsible for protein-primed viral DNA replication by strand displacement with high processivity and fidelity. Possesses three enzymatic activities: DNA synthesis (polymerase), primer terminal protein (TP) deoxynucleotidylation, and 3' to 5' exonuclease activity.
    Depositor
    Jennifer Molloy, FreeGenes Project, Drew Endy
    Insert
    phi29 DNA Polymerase
    Use
    Synthetic Biology
    Available Since
    Aug. 24, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165573-plasmid-map-sequence-id-340935

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  14. USP2

    Plasmid
    #36894
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    USP2 (USP2 Human)
    Tags
    His tag with thrombin cleavage
    Expression
    Bacterial
    Available Since
    June 19, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  15. USP15

    Plasmid
    #28282
    Depositor
    Cheryl Arrowsmith
    Insert
    USP15 (USP15 Human)
    Tags
    His tag with thrombin cleavage
    Expression
    Bacterial
    Mutation
    Contains dusp and Ubl domains (residues 6-223).
    Available Since
    April 14, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  16. pET His6 GFP TEV LIC cloning vector (1GFP)

    Plasmid
    #29663
    Depositor
    Scott Gradia
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Tags
    6xHis, GFP, and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available Since
    June 7, 2011
    Availability
    Academic Institutions and Nonprofits only
    29663-plasmid-map-sequence-id-197189

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  17. pHR-mCh-Cry2WT_NLS

    Plasmid
    #221925
    Purpose
    Expression of SV40 nuclear localization signal (NLS) fused to optogenetic protein mCh-Cry2WT
    Depositor
    Daniel Bose
    Insert
    SV40 NLS
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    insertion of SV40 NLS
    Available Since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    221925-plasmid-map-sequence-id-437713

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  18. Hsp68-LacZ-Gibson

    Plasmid
    #170102
    Purpose
    Transgenic LacZ reporter construct for validation of enhancer sequences (inserted via Gibson-cloning). Can be introduced into the mouse genome via random integration following pro-nuclear injection.
    Depositor
    Len Pennacchio
    Type
    Empty backbone
    Use
    Mouse Targeting
    Promoter
    Mouse Hsp68 minimal promoter
    Available Since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    170102-plasmid-map-sequence-id-346308

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  19. pET tdTomato LIC cloning vector (H6-tdTomato)

    Plasmid
    #86928
    Purpose
    pET LIC cloning vector for Biotin-His6-tev-yORF-tdTomato
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Tags
    6xHis, AviTag (biotin), TEV cleavage site, and td…
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    86928-plasmid-map-sequence-id-175058

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  20. Cre Shine

    Plasmid
    #37404
    Purpose
    This is a 2A peptide construct that produces two different proteins from a single open reading frame, a nuclear targeted Cre recombinase, and a membrane targeted GFP.
    Depositor
    Thomas Hughes
    Insert
    Cre Shine
    Use
    Cre/Lox
    Tags
    eGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 1, 2012
    Availability
    Academic Institutions and Nonprofits only
    37404-plasmid-map-sequence-id-114840

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Showing: 8581 - 8600 of 15735 results