We narrowed to 88,248 results for: MAL

1. pePEmax-pPuro

   Plasmid

   #215688

   Purpose

   Cloning vector for Uni-Vector prime editing system

   Depositor

   Kou-Juey Wu

   Article

   Huang et al Biotechnol J. 2024 Feb;19(2):e2300353. d

   Insert

   PEmax
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   EFS promoter

   Available Since

   Dec. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Tom70 (1-70)-GFP

   Plasmid

   #127633

   Purpose

   Mitochondrial targeting sequence of Tom70 fused to GFP

   Depositor

   Josef Kittler

   Article

   Covill-Cooke et al EMBO Rep. 2020 Feb 5;21(2):e49865. doi:

   Insert

   Tom70 (TOMM70 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Only first 70 amino acids

   Promoter

   Available Since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAS_4xMx PylT_GFP 150TAG

   Plasmid

   #140016

   Purpose

   Plasmid with 4xMxPylT cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

   Insert

   sfGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   150TAG, K46E (Please see depositor comments below)

   Promoter

   EF1

   Available Since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR-CMV-TetO2_3C-Avi-His6_IRES-mRuby2

   Plasmid

   #113889

   Purpose

   Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of cytosolic mRuby2

   Depositor

   A. Radu Aricescu

   Article

   Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-Avi-His6

   Expression

   Mutation

   Promoter

   CMV-MIE-TetO2

   Available Since

   Aug. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. CBE-T1.14

   Plasmid

   #193277

   Purpose

   Expression of CBE-T1.14 base editor in mammalian cells, CMV promoter

   Depositor

   Nicole Gaudelli

   Article

   Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

   Insert

   CBE-T1.14
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D10A (Cas9), TadA mutations within

   Promoter

   CMV

   Available Since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. TRE-VEGFA EF1a-rtTA3

   Plasmid

   #195881

   Purpose

   Lentiviral construct of doxycycline inducible VEGFA

   Depositor

   Elaine Fuchs

   Article

   Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

   Insert

   VEGFA (Vegfa Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3GS

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRK5-FLAG-ATP6V0D1

   Plasmid

   #87931

   Purpose

   FLAG-tagged overexpression

   Depositor

   David Sabatini

   Article

   Zoncu et al Science. 2011 Nov 4;334(6056):678-83.

   Insert

   ATP6Vod1 (ATP6V0D1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3 Lats1 (Nigg HS189)

   Plasmid

   #41156

   Depositor

   Erich Nigg

   Article

   Chan et al Oncogene. 2005 Mar 17;24(12):2076-86.

   Insert

   Lats1 (LATS1 Human)
   

   Use

   Tags

   3xMyc and PreScission Site

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 7, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pAAV-TRE-FLEX-eNpHR-EYFP

   Plasmid

   #89876

   Purpose

   To generate AAV virus that will express eNpHR-EYFP in Cre-dependent recombination under control of tetO promoter

   Depositor

   Hyungbae Kwon

   Article

   Lee et al Nat Methods. 2017 May;14(5):495-503. doi

   Insert

   eNpHR=EYFP
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   TetO

   Available Since

   May 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. Mucolipin1-pEGFP C3

    Plasmid

    #62960

    Purpose

    full length human Mucolipin-1 cloned into pEGFP C3

    Depositor

    Paul Luzio

    Article

    Pryor et al Traffic. 2006 Oct;7(10):1388-98.

    Insert

    Mucolipin-1 (MCOLN1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Mucolipin-1 has 1 silent mutation compared to the…

    Promoter

    CMV

    Available Since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pRK5-HA-Sestrin2

    Plasmid

    #72593

    Purpose

    overexpression, codon optimized for mammalian expression

    Depositor

    David Sabatini

    Article

    Chantranupong et al Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1

    Insert

    SESN2 (SESN2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    codon optimized for mammalian expression, see dep…

    Promoter

    CMV

    Available Since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pCK351

    Plasmid

    #129696

    Purpose

    For insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + terminator ECK120034435

    Depositor

    John Heap

    Article

    Kelly et al Microorganisms. 2019 Aug 16;7(8). pii: m

    Inserts

    PrhaBAD
    yfp
    rhaS
    Terminator ECK120034435
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. GST-BHMT-I/G

    Plasmid

    #104442

    Purpose

    Autophagy cargo assay

    Depositor

    Carol Mercer

    Article

    Mercer et al Autophagy. 2008 Feb;4(2):185-94. Epub 20

    Insert

    BHMT (BHMT Human)
    

    Use

    Tags

    GFP-myc GFP is from Aequorea victoria and GST

    Expression

    Mammalian

    Mutation

    N43A in GST (in a potential KFERQ-like sequence).…

    Promoter

    CMV

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. CROPseq-Guide-BSD

    Plasmid

    #216123

    Purpose

    Makes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the blasticidin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.

    Depositor

    Stefan Kubicek

    Article

    Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. SMC2 CRISPR

    Plasmid

    #140649

    Purpose

    SMC2 tagging CRISPR

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    SMC2 (SMC2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMK312 (TOP2A CRISPR)

    Plasmid

    #140654

    Purpose

    TOP2A tagging CRISPR

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    TOP2A (TOP2A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. 12x PRE TK luc

    Plasmid

    #206163

    Purpose

    Luciferase reporter construct containing 12 consensus PGR binding sites upstream of a minimal TK promoter, derived from Addgene #11350

    Depositor

    Renee van Amerongen

    Article

    Aarts et al J Mammary Gland Biol Neoplasia. 2023 Nov

    Insert

    12X PRE TK luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    min TK

    Available Since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-hSyn-FLEX-RiboL1-jGCaMP8f

    Plasmid

    #192618

    Purpose

    AAV transfer plasmid for neuronal expression of Cre-dependent (FLEX), soma-targeted (RiboL1) jGCaMP8f.

    Depositor

    Marianne Fyhn

    Article

    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

    Insert

    FLEX-RiboL1-jGCaMP8f
    

    Use

    AAV and Cre/Lox

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMK395 (AAVS1 CMV-OsTIR1(F74A))

    Plasmid

    #159992

    Purpose

    Expression of OsTIR1(F74A) in human cells

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    CMV-OsTIR1(F74A)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. Nurr1

    Plasmid

    #35000

    Depositor

    Malin Parmar

    Article

    Pfisterer et al Proc Natl Acad Sci U S A. 2011 Jun 21;10

    Insert

    Nuclear receptor subfamily 4 group A member 2 (Nr4a2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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