We narrowed to 8,622 results for: PAN

1. pCAGGS-HaloTag-KRas4B(CT)

   Plasmid

   #214276

   Purpose

   Mammalian expression of HaloTag fused to the C-terminal tail of KRas4B

   Depositor

   Shinya Tsukiji

   Article

   Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

   Insert

   HaloTag7-KRas4B(174-188) (KRAS Human)
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Shank1 and Shank3 scFv [N367/51]

   Plasmid

   #206728

   Purpose

   Mammalian Expression of Shank1 and Shank3 scaffold protein scFV. Derived from hybridoma N367/51 scFv.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   Shank1 and Shank3 scaffold protein (Rattus norvegicus) recombinant scFV (Shank3 Mouse)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMEXneo-TrkC-Δ6

   Plasmid

   #190204

   Purpose

   To express the human TrkC that has in the extracellular domain only the second IgG like domain. Still binds NT-3.

   Depositor

   Pantelis Tsoulfas

   Article

   Urfer et al J Biol Chem. 1998 Mar 6;273(10):5829-40.

   Insert

   TrkC (NTRK3 Human)
   

   Expression

   Mammalian

   Mutation

   The extracellualr domain conatins only the signal…

   Promoter

   Moloney mouse sarcoma virus right LTR

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAGGS-chGrb2/eDHFR(69K6)-iRFP713

   Plasmid

   #178854

   Purpose

   Mammalian expression of Grb2-eDHFR(69K6) chimera fused to iRFP713

   Depositor

   Shinya Tsukiji

   Article

   Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

   Insert

   Grb2(1-59)-eDHFR(69K6)-Grb2(152-216)-iRFP713 (GRB2 Human)
   

   Expression

   Mammalian

   Mutation

   Grb2: Y160E

   Promoter

   CAG

   Available Since

   July 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR201-JIP60ml

   Plasmid

   #104959

   Purpose

   Encodes a modified, active, form of Hordeum vulgare c.v. Golden Promise Jasmonate Induced Protein 60 (JIP60)

   Depositor

   Pietro Spanu

   Article

   Pennington et et al Figshare (2018)

   Insert

   Jasmonate Induced Protein 60 (JIP60)
   

   Use

   Gateway entry vector for lr recombination into ot…

   Mutation

   Replaced amino acids 164 to 187 with a methionine…

   Available Since

   March 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. CSKP_HUMAN_D0

   Plasmid

   #79714

   Purpose

   This plasmid encodes the kinase domain of CSKP. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   John Chodera, Nicholas Levinson, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   CSKP (CASK Human)
   

   Tags

   His10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pKT2/CAGXSP/CD63mScarlet

   Plasmid

   #182972

   Purpose

   Stably express CD63 fused with mScarlet in mammalian cells via the sleeping beauty transposon system.

   Depositor

   Masamitsu Kanada

   Article

   Perez et al Adv Genet (Hoboken). 2022 Jan 19;3(1):21

   Insert

   CD63-mScarlet (CD63 Human)
   

   Expression

   Mammalian

   Mutation

   N/A

   Available Since

   April 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)-HA

   Plasmid

   #182653

   Purpose

   Encodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF),tRNACUAPyl & eRF1E55D used for amber codon suppression in mammalian cells

   Depositor

   Ivana Nikić-Spiegel

   Article

   Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

   Inserts

   codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
   PylT
   mutant eukaryotic release factor 1
   

   Tags

   HA tag and nuclear export signal (NES)

   Expression

   Mammalian

   Mutation

   E55D and Y306A/Y384F (AF) double mutant of Methan…

   Promoter

   CMV and U6

   Available Since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. EF1a-L274GMmMetRS-T2A-mCherry

   Plasmid

   #220800

   Purpose

   Lentivirus expressing mutant tRNA synthetase for incorporation of noncanonical amino acids in nascent proteins plus mCherry reporter and puro resistance

   Depositor

   Elizabeth Kirby

   Article

   Sridharan et al Stem Cell Res Ther. 2024 Jun 26;15(1):18

   Insert

   Mars1 (Mars1 Mouse)
   

   Use

   Lentiviral

   Tags

   2xFLAG and T2A-mCherry

   Mutation

   mutation in MetRS to change aa 274 from L to G

   Promoter

   EF1a

   Available Since

   June 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAcBac3-pAAFRS-8xYtR-EGFP-3TAA- 40TGA-152TAG-TEV-3xUAA

    Plasmid

    #228788

    Purpose

    EcTyr aaRS/tRNA system for pAAF incorporation with EGFP containing three stop codons for triple suppression

    Depositor

    Abhishek Chatterjee

    Article

    Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

    Inserts

    EcTyrRS
    EcYtR
    EGFP***
    TEV protease
    

    Use

    Baculoviral

    Expression

    Mammalian

    Mutation

    TAG suppressor

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPaGE Pyl TAG FliC T248TAG

    Plasmid

    #160089

    Purpose

    Encodes for MmPyl tRNA synthetase/tRNA pair and for flagellin protein (FliC) with TAG mutation at site 248, for unnatural amino acid incorporation into the flagellin protein in Pseudomonas aeruginosa.

    Depositor

    Lital Alfonta

    Article

    Ozer et al Sci Adv. 2021 Jun 11;7(24). pii: 7/24/ea

    Inserts

    Pyrrolysyl tRNA(cua) (Methanosarcina mazei)
    Pyrrolysyl tRNA synthetase (Methanosarcina mazei)
    Flagellin
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Changed Threonine 248 to TAG stop-codon.

    Promoter

    Pseudomonas aeruginosa Leu-tRNA native promoter a…

    Available Since

    June 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR223_DNAJC12_WT

    Plasmid

    #81974

    Purpose

    Gateway Donor vector containing DNAJC12, part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    DNAJC12 (DNAJC12 Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMEXneo-TrkC-TrkB-hybrid

    Plasmid

    #190205

    Purpose

    To express a Human TrkC receptor that binds only BDNF. The second IgG like domain which is the main binding domain comes from the TrkB receptor

    Depositor

    Pantelis Tsoulfas

    Article

    Urfer et al J Biol Chem. 1998 Mar 6;273(10):5829-40.

    Insert

    TrkC (NTRK3 Human)
    

    Expression

    Mammalian

    Mutation

    The second IgG like domain and the extracellular …

    Promoter

    Moloney mouse sarcoma virus right LTR

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. MK01_HUMAN_D0

    Plasmid

    #79713

    Purpose

    This plasmid encodes the kinase domain of MK01. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    MK01 (MAPK1 Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pAcBac3-OMeYRS-8xYtR-EGFP-3TAA-40TGA-152TAG-TEV-3xUAA

    Plasmid

    #228787

    Purpose

    EcTyr aaRS/tRNA system for TAG suppression with EGFP containing three stop codons for triple suppression

    Depositor

    Abhishek Chatterjee

    Article

    Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

    Inserts

    EcTyrRS
    EcYtR
    EGFP***
    TEV protease
    

    Use

    Baculoviral

    Expression

    Mammalian

    Mutation

    TAG suppressor

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. Anti-Shank2 [N23B/69R]

    Plasmid

    #225382

    Purpose

    Mammalian Expression Plasmid of anti-Shank2 (Rat) IgG2a R-mAb. Derived from hybridoma N23B/69.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    Anti-Shank2 (Rattus norvegicus) recombinant (Mouse) monoclonal antibody. (Shank2 Mouse)
    

    Expression

    Mammalian

    Promoter

    Dual CMV

    Available Since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. Anti-Shank2 [N23B/6R-2b]

    Plasmid

    #219475

    Purpose

    Mammalian Expression Plasmid of anti-Shank2 scaffold protein (Rat) subclass-switched IgG2b R-mAb. Derived from hybridoma N23B/6.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    Anti-Shank2 scaffold protein (Rattus norvegicus) recombinant mouse monoclonal antibody. (Shank2 Mouse)
    

    Expression

    Mammalian

    Promoter

    Dual CMV

    Available Since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. Anti-Shank3 [N69/38R]

    Plasmid

    #219458

    Purpose

    Mammalian Expression Plasmid of anti-Shank3 (Rat) IgG2a R-mAb. Derived from hybridoma N69/38.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    Anti-Shank3 (Rattus norvegicus) recombinant mouse monoclonal antibody. (Shank3 Mouse)
    

    Expression

    Mammalian

    Promoter

    Dual CMV

    Available Since

    July 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. Anti-Shank3 [N69/46R-2b]

    Plasmid

    #206675

    Purpose

    Mammalian Expression Plasmid of anti-Shank3 (Rat). Derived from hybridoma N69/46-2b.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-Shank3 (Rattus norvegicus) recombinant Mouse monoclonal antibody (Shank3 Mouse)
    

    Expression

    Mammalian

    Promoter

    Dual CMV

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pD649-HAsp-CNTN3-COMP5AP-AviTag-9xHis

    Plasmid

    #157582

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    CNTN3 (CNTN3 Human)
    

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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