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pPD135 ZF43x4-Spaced-Mid_EYFP

Plasmid

#138885

Purpose

EYFP reporter vector for the ZF43x4-Spaced-Mid promoter

Depositor

Joshua Leonard

Publication

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x4-Spaced-Mid

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x4-Spaced-Mid

Available since

Jan. 31, 2021

Availability

Academic Institutions and Nonprofits only

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pPD134 ZF43x2-Spaced-Mid_EYFP

Plasmid

#138884

Purpose

EYFP reporter vector for the ZF43x2-Spaced-Mid promoter

Depositor

Joshua Leonard

Publication

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x2-Spaced-Mid

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x2-Spaced-Mid

Available since

Jan. 31, 2021

Availability

Academic Institutions and Nonprofits only

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pHIE065 ZF43x12-S mKate2 EF1a EBFP2-P2A-BlastR

Plasmid

#138746

Purpose

Integration Vector, assembled with pHIE049 (TUPV1), pJM451 (TUPV2), pPD612

Depositor

Joshua Leonard

Publication

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x12-Spaced

Use

Synthetic Biology

Expression

Mammalian

Available since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pPD336 VPR-ZF43(RAAR)

Plasmid

#138823

Purpose

Constitutive expression of VPR-ZF43(RAAR) under the CMV promoter.

Depositor

Joshua Leonard

Publication

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VPR-ZF43(RAAR)

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Mutation

R15A, R43A

Promoter

CMV

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pPD111 VP16-ZF43(AARR)

Plasmid

#138816

Purpose

Constitutive expression of VP16-ZF43(AARR) under the CMV promoter.

Depositor

Joshua Leonard

Publication

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VP16-ZF43(AARR)

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Mutation

R6A, R15A

Promoter

CMV

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pPD108 VP16-ZF43(RAAR)

Plasmid

#138813

Purpose

Constitutive expression of VP16-ZF43(RAAR) under the CMV promoter.

Depositor

Joshua Leonard

Publication

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VP16-ZF43(RAAR)

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Mutation

R15A, R43A

Promoter

CMV

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pHIE060 ZF43x3-C mKate2 EF1a EBFP2-P2A-BlastR

Plasmid

#138741

Purpose

Integration Vector, assembled with pHIE044 (TUPV1), pJM451 (TUPV2), pPD612

Depositor

Joshua Leonard

Publication

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x3-Compact

Use

Synthetic Biology

Expression

Mammalian

Available since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pHIE063 ZF43x3-S mKate2 EF1a EBFP2-P2A-BlastR

Plasmid

#138744

Purpose

Integration Vector, assembled with pHIE047 (TUPV1), pJM451 (TUPV2), pPD612

Depositor

Joshua Leonard

Publication

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x3-Spaced

Use

Synthetic Biology

Expression

Mammalian

Available since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pHIE064 ZF43x6-S mKate2 EF1a EBFP2-P2A-BlastR

Plasmid

#138745

Purpose

Integration Vector, assembled with pHIE048 (TUPV1), pJM451 (TUPV2), pPD612

Depositor

Joshua Leonard

Publication

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x6-Spaced

Use

Synthetic Biology

Expression

Mammalian

Available since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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A102-Par-BoCCS-GRM

Plasmid

#123289

Purpose

Expression of truncated Glo3 (296-459) in E.Coli

Depositor

Blanche Schwappach

Publication

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Glo3 (GLO3 Budding Yeast)

Tags

His and MBP

Expression

Bacterial

Mutation

296-459

Promoter

T5

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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LCV2_AAVS1_sgRNA_4

Plasmid

#155090

Purpose

lentiviral plasmid expressing Cas9 and gRNA targeting AAVS1 safe harbor locus

Depositor

Jason Moffat

Publication

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

AAVS1_sgRNA_4

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter

Available since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Orf6

Plasmid

#157726

Purpose

mammalian expression of untagged SARS-CoV-2 Orf6 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Publication

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Orf6 (ORF6 SARS-CoV-2)

Expression

Mammalian

Mutation

codon optimized

Available since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT-TO-Nsp15

Plasmid

#157720

Purpose

mammalian expression of untagged SARS-CoV-2 Nsp15 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Publication

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp15 (ORF1ab SARS-CoV-2)

Expression

Mammalian

Mutation

codon optimized

Available since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT-TO-Nsp14

Plasmid

#157717

Purpose

mammalian expression of untagged SARS-CoV-2 Nsp14 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Publication

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp14 (ORF1ab SARS-CoV-2)

Expression

Mammalian

Mutation

codon optimized

Available since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3-CBX1mut-Dronpa

Plasmid

#138250

Purpose

Triple-mutant CBX1 chromodomain (residues 20-73) tagged with reversibly switchable FP Dronpa. Mutations V3E/K6E/D40S correspond to positions V22/K25/D59 in full-length CBX1.

Depositor

Jin Zhang

Publication

Hard et al Sci Adv. 2018 Nov 7;4(11):eaau1447. doi:

Insert

CBX1mut-Dronpa

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Nod-BFP2-TSERex

Plasmid

#124785

Purpose

Expresses the proton pump rhodopsin in mammalian cells under CAG promoter

Depositor

Yulong Li

Publication

Wu et al Elife. 2019 Jan 14;8. pii: 43366. doi: 1

Insert

Nod-BFP2

Expression

Mammalian

Promoter

CAG

Available since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pBAD-MTX_Snifit

Plasmid

#119043

Purpose

Plasmid encoding the bacterial expression of the control Snifit-MTX (FRET semi-synthetic biosensor)

Depositor

Kai Johnsson

Publication

Xue et al J Am Chem Soc. 2016 Apr 27;138(16):5258-

Insert

MTX_Snifit

Tags

His-tag and Strep-tag

Expression

Bacterial

Mutation

R44L H45Q

Promoter

pBAD

Available since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pTG-mTDGa.0

Plasmid

#81048

Purpose

bacterial expression of murine TDG

Depositor

Primo Schaer

Publication

Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

Insert

TDG (Tdg Mouse)

Tags

GST

Expression

Bacterial

Promoter

T7

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-PDAE

Plasmid

#110180

Purpose

Expresses propanediol dehydratase activating enzyme in E. coli BL21(DE3)

Depositor

Emily Balskus

Publication

Levin et al Science. 2017 Feb 10;355(6325). pii: 355

Insert

Propanediol Dehydratase Activating Enzyme

Tags

His6

Expression

Bacterial

Promoter

T7

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-P5CR

Plasmid

#110295

Purpose

Expresses pyrroline-5-carboxylate reductase in E. coli BL21(DE3)

Depositor

Emily Balskus

Publication

Levin et al Science. 2017 Feb 10;355(6325). pii: 355

Insert

Pyrroline-5-carboxylate Reductase

Tags

His6

Expression

Bacterial

Promoter

T7

Available since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

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