Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 15,990 results for: nes

Showing: 8601 - 8620 of 15990 results

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Enterovirus A71 (EV-A71) isolate TW-2553-1998 3C protease

Plasmid

#228636

Purpose

Bacterial expression of the A71 3C protease (isoform B) used in crystallisation

Depositor

ASAP Discovery, Frank von Delft

Insert

Enterovirus A71 3C protease isoform B

Tags

His-SUMO

Expression

Bacterial

Available Since

Feb. 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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AiP13591 - pAAV-AiE0512h_3xC3-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3591)

Plasmid

#229913

Purpose

AiE0512h_3xC3 is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific brain cell types

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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AiP13152 - pAAV-AiE0050m_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN3152)

Plasmid

#224056

Purpose

AiE0050m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L4/5_IT neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

iCre(R297T)

Use

AAV

Mutation

NA

Promoter

minBG

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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AiP12612 - pAAV-AiE0772m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2612)

Plasmid

#224000

Purpose

AiE0772m is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L6_IT_Car3 neurons (note CLA neurons)

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Mutation

NA

Promoter

minBG

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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AiP13029 - pAAV-AiE0462m_3xC1-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3029)

Plasmid

#224023

Purpose

AiE0462m_3xC1 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L5_ET neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Mutation

NA

Promoter

minBG

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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AiP13028 - pAAV-AiE0461m_3xC3-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3028)

Plasmid

#224022

Purpose

AiE0461m_3xC3 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L5_ET neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Mutation

NA

Promoter

minBG

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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AiP14908 - pAAV-AiE0354h-minBG-mScarlet-WPRE3-BGHpA (Alias: CN4908)

Plasmid

#224052

Purpose

AiE0354h is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical Vip+ neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.

Insert

mScarlet

Use

AAV

Mutation

NA

Promoter

minBG

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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AiP13583 - pAAV-AiE0475m_3xC1-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3583)

Plasmid

#224030

Purpose

AiE0475m_3xC1 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical Pvalb+ neurons (sparse ChC cells)

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Mutation

NA

Promoter

minBG

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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AiP12944 - pAAV-AiE0667m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2944)

Plasmid

#224017

Purpose

AiE0667m is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L5_ET_Chrna6 neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Mutation

NA

Promoter

minBG

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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AiP12863 - pAAV-AiE0724m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2863)

Plasmid

#224015

Purpose

AiE0724m is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical Lamp5 GABAergic neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Mutation

NA

Promoter

minBG

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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AiP12653 - pAAV-AiE0720h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2653)

Plasmid

#224001

Purpose

AiE0720h is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L4/5_IT neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Mutation

NA

Promoter

minBG

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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AiP14480 - pAAV-AiE2118m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN4480)

Plasmid

#224046

Purpose

AiE2118m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in forebrain astrocytes

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Mutation

NA

Promoter

minBG

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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AiP14668 - pAAV-AiE0724m-minBG-iCre(R297T)-BGHpA (Alias: CN4668)

Plasmid

#224051

Purpose

AiE0724m is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical Lamp5 GABAergic neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

iCre(R297T)

Use

AAV

Mutation

NA

Promoter

minBG

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pSG058

Plasmid

#214261

Purpose

PaFtsH1-6xHis cloned between XbaI/NheI in pET-28a(+)

Depositor

Ute Romling

Article

Mawla et al J Biol Chem. 2024 Feb;300(2):105622. doi

Insert

Core genome PaFtsH1 proteinase from Pseudomonas aeruginosa SG17M

Tags

6xHis-tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pBABE-puro-Halo-hMRE11(wt)

Plasmid

#208393

Purpose

To generate stable cell line expressing Halo-human MRE11(WT) by Retroviral infection.

Depositor

Gaorav Gupta

Article

Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

Insert

Human MRE11 (MRE11 Human)

Use

Luciferase and Retroviral

Expression

Mammalian

Mutation

H129N

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti-sgRNA-Cre-GpNLuc-sgmcGAS

Plasmid

#208385

Purpose

Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine cGAS gene.

Depositor

Gaorav Gupta

Article

Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

Insert

sgcGAS (Cgas Mouse)

Use

Lentiviral and Luciferase

Expression

Mammalian

Mutation

WT

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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U6-CjCas9-MS2gRNA-Backbone

Plasmid

#210711

Purpose

This plasmid express MS2 loop containing Cj Cas9 gRNA

Depositor

Isaac Hilton

Article

Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

Insert

MS2 loop containing Cj Cas9 gRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1-V5-HisB-ObKHC-wt-HaloTag

Plasmid

#201551

Purpose

Expresses wild-type Octopus kinesin-1 motor with HaloTag in Drosophila S2 cells

Depositor

Joshua Rosenthal

Article

Birk et al Cell. 2023 Jun 8;186(12):2544-2555.e13.

Insert

Kinesin-1 (partial)

Tags

HaloTag

Expression

Insect

Mutation

Codon-optimized for Drosophila expression

Promoter

Ac5

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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TRE-UBC-mCD59-T2A-GFP

Plasmid

#205451

Purpose

lentiviral plasmid for expression of mouse CD59

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

mCD59 (Cd59a Mouse)

Expression

Mammalian

Promoter

UBC

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TIE-V5/HIS

Plasmid

#205612

Purpose

Expression of human TIE receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

TIE receptor tyrosine kinase (TIE1 Human)

Tags

V5-His

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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Showing: 8601 - 8620 of 15990 results

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