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pcDNA3-mitoExRai-AKAR2

Plasmid

#161757

Purpose

ExRai-AKAR2 targeted to the outer mitochondrial membrane.

Depositor

Jin Zhang

Article

Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

Insert

DAKAP-ExRai-AKAR2

Tags

N-terminal 30 amino acids from DAKAP1

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pv9_CBX1_2xChromo-13XL-BASU

Plasmid

#179412

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX1.2xChromo-13XL-BASU

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

CBX1.2xChromo-13XL-BASU

Expression

Mammalian

Promoter

CAGGS

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ262-ABE8e

Plasmid

#161523

Purpose

Gateway entry clone (attL1 & attR5) for CRISPR-zSpCas9 ABE8e mediated A-G base editing

Depositor

Yiping Qi

Article

Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41

Insert

ABE8e-zSpCas9(D10A)

Use

CRISPR; Gateway compatible abe8e-zspcas9(d10a) en…

Expression

Plant

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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HP-Cas9hGem-Ade2-LEU2-LINEAR

Plasmid

#174839

Purpose

pRS414 backbone containing LINEAR fragment targeting ADE2 in H. polymorpha

Depositor

Zengyi Shao

Article

Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

Insert

HH-gRNA-HDV targetting ADE2 in Ogataea (para)polymorpha

Use

CRISPR and Synthetic Biology

Expression

Bacterial and Yeast

Promoter

TDH3p

Available Since

Jan. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pClneoMyc human ERBIN

Plasmid

#40214

Depositor

Yutaka Hata

Article

Ohno et al Oncogene. 2002 Oct 10;21(46):7042-9.

Insert

ERBIN (ERBIN Human)

Tags

myc

Expression

Mammalian

Mutation

L143S, S274L and P338L mutation in ERBIN compared…

Promoter

CMV

Available Since

Sept. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pSN16

Plasmid

#100103

Purpose

expresses a chimeric OCT4-activator fusion

Depositor

Mark Ptashne

Article

Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

Insert

vexGFP-P2A-OCT4 fused to a 'strong' VP16 domain (POU5F1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pLIB-WDR61

Plasmid

#231729

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

WDR61 (SKIC8 Human)

Expression

Insect

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pEF-Bos-FLAG MDA5 MI

Plasmid

#52875

Purpose

Expresses MDA5 with a mutation to motif I in mammalian cells

Depositor

Curt Horvath

Article

Bamming et al J Biol Chem. 2009 Apr 10;284(15):9700-12

Insert

IFIH1 (IFIH1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

K335A, E658K

Available Since

May 9, 2014

Availability

Academic Institutions and Nonprofits only

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pMIR-DsRed-IRES-His-Halo-Tev-Keap1

Plasmid

#58240

Purpose

expresses halo tagged human Keap1 protein

Depositor

Yimon Aye

Article

Fang et al J Am Chem Soc. 2013 Oct 2;135(39):14496-

Insert

Kelch like ECH associated protein 1 (KEAP1 Human)

Tags

His-Halo-Tev

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-Flag-hNF2

Plasmid

#107150

Purpose

mammalian expression

Depositor

Tina Izard

Article

Chinthalapudi et al Nat Commun. 2018 Apr 6;9(1):1338. doi: 1

Insert

neurofibromin 2 (NF2 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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