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pEGFP_sfCherry2(11)_H2B

Plasmid

#82605

Purpose

Expresses sfCherry2(11) tagged H2B in mammalian cells

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

sfCherry2(11)_H2B

Expression

Mammalian

Mutation

changed Gly12 to Ala in sfCherry11+

Available Since

June 29, 2017

Availability

Academic Institutions and Nonprofits only

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WNK2 WT

Plasmid

#24569

Depositor

Joseph Costello

Article

Hong et al Proc Natl Acad Sci U S A. 2007 Jun 26. 1

Insert

WNK2 (WNK2 Human)

Tags

His6 and V5

Expression

Mammalian

Available Since

May 20, 2010

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR_Puro_sgAAVS1

Plasmid

#187818

Purpose

pLentiCRISPR that is selectable with puromycin with an sgRNA targeting AAVS1

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

sgAAVS1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pMP71-CD40L

Plasmid

#174595

Purpose

Stably expresses the extracellular and transmembrane domain of murine CD40L

Depositor

Stanley Riddell

Article

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Insert

CD40L

Expression

Mammalian

Available Since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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HPV16 E6 TCR

Plasmid

#122727

Purpose

Express HPV16 E6 TCR

Depositor

Christian Hinrichs

Article

Draper et al Clin Cancer Res. 2015 Oct 1;21(19):4431-

Insert

HPV16 E6 TCR

Use

Retroviral

Available Since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

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pK036.TRE-Flpe-WPRE (Supernova)

Plasmid

#85007

Purpose

For Flpe/FRT-based Supernova, pK036 should be used with pK068 etc.

Depositor

Takuji Iwasato

Article

Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

Insert

NLS-FLPe-WPRE

Expression

Mammalian

Available Since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

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MSCVhygro-F-G9a

Plasmid

#41721

Purpose

Retroviral expression of human G9a in mammalian cells

Depositor

Kai Ge

Article

Wang et al EMBO J. 2012 Nov 23. doi: 10.1038/emboj.

Insert

G9a (EHMT2 Human)

Use

Retroviral

Tags

flag

Expression

Mammalian

Available Since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only

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NW11

Plasmid

#133443

Purpose

Covalently circularized nanodisc NW11 for studying membrane proteins and viral entry.

Depositor

Gerhard Wagner

Article

Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

Insert

NW11

Expression

Bacterial

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-TagGFP2

Plasmid

#121426

Purpose

TAgGFP2 was amplified from pLenti-Origene-Nrf21 and inserted into XhoI and EcoRI digested pLenti-Origene-Nrf21.

Depositor

Ovijit Chaudhuri, Stanley Qi

Article

Lee et al Nat Commun. 2019 Apr 23;10(1):1848. doi:

Insert

empty TagGFP2

Use

Lentiviral

Tags

TagGFP2

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-mROCK2

Plasmid

#101296

Purpose

Mammalian expression of Rock2-GFP

Depositor

Alpha Yap

Article

Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

Insert

Rock2 (Rock2 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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human STIM1 YFP

Plasmid

#19754

Depositor

Anjana Rao

Article

Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

Insert

STIM1 (STIM1 Human)

Use

Retroviral

Tags

YFP

Expression

Mammalian

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only

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pBMN-dGBP1-TagBFP

Plasmid

#80087

Purpose

MMLV retroviral vector for expression of dGBP1-TagBFP with t-hcRed as an infection/transfection marker

Depositor

Connie Cepko

Article

Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

Insert

dGBP1-TagBFP

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 17, 2018

Availability

Academic Institutions and Nonprofits only

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cfSGFP2-anti-AlfaTag nanobody

Plasmid

#171818

Purpose

Bacterial expression vector for production of fluorescent anti-AlfaTag nanobody

Depositor

Ikuo Wada

Article

Matsumoto et al J Biol Chem. 2021 Jul;297(1):100851. doi

Insert

cfSGFP2-anti-AlfaTag nanobody

Tags

6xHis and SEP tag (6xD)

Expression

Bacterial

Available Since

June 27, 2022

Availability

Academic Institutions and Nonprofits only

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Kras-Src FRET biosensor

Plasmid

#78302

Purpose

FRET biosensor. To monitor Src kinase activity in mammalian cells by FRET

Depositor

Yingxiao Wang

Article

Ouyang et al Proc Natl Acad Sci U S A. 2008 Sep 23;10

Insert

Src biosensor

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5 HIS-Ub-K48R

Plasmid

#221368

Purpose

Expresses 6xHIS-tagged Ubiquitin containing K48R mutation in mammalian cells

Depositor

Aga Gambus

Article

Jones et al iScience. 2024 Jun 12;27(7):110260. doi:

Insert

Ubiquitin

Tags

6xHIS

Expression

Mammalian

Mutation

Changed Lysine 48 to Arginine

Promoter

CMV

Available Since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pZyxin-mMaple3

Plasmid

#101151

Purpose

Fluorescent protein for superresolution imaging and a focal adhesion protein for binding substrate

Depositor

Xiaowei Zhuang

Article

Wang et al Proc Natl Acad Sci U S A. 2014 Jun 10;11

Insert

human codon optimized mMaple3 fused to zyxin

Expression

Mammalian

Promoter

cmv

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pDonr201_PARG WT

Plasmid

#240315

Purpose

Entry vector for Gateway with PARG

Depositor

Junjie Chen

Article

Nie et al Elife. 2024 Apr 5;12:RP89303. doi: 10.75

Insert

PARG (PARG Human)

Expression

Bacterial

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pFastBac-Dual- GST-TEV-TBK1

Plasmid

#208875

Purpose

For expression in insect cells of TBK1

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

TBK1 (TBK1 Human)

Tags

GST-TEV

Expression

Insect

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pX330-BsaIx2-DD-Cas9

Plasmid

#75380

Purpose

Expresses DD-SpCas9 in mammalian cells and has a cloning site for an sgRNA. The FKBP12 L106P destabilization domain allows for inducible stabilization of Cas9 through the addition of Shield-1

Depositor

Michele Calos

Article

Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv

Insert

DD-SpCas9

Use

CRISPR

Tags

FKBP12 L106P DD

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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p3E-FRT-KanR-FRT pA

Plasmid

#82601

Purpose

3' entry vector containing FRT-flanked kanamycin resistance cassette (KanR) with pA for FLP-induced kanamycin resistance, drug selection

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

FRT-Kan-FRT

Promoter

None

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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