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CCNL1

Plasmid

#155440

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

CCNL1 (CCNL1 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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BTG4

Plasmid

#155428

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

BTG4 (BTG4 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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BTG3

Plasmid

#155427

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

BTG3 (BTG3 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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CPSF4L

Plasmid

#155479

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

CPSF4L (CPSF4L Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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SFRS17A-1

Plasmid

#156011

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

SFRS17A (SFRS17A Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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EXOSC2

Plasmid

#155590

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

EXOSC2 (EXOSC2 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-f1(+)-TOMM20-mEGFP

Plasmid

#115911

Purpose

donor to insert EGFP at the TOMM20 locus in human cells

Depositor

Erik Sontheimer

Article

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

TOMM20-mEGFP (TOMM20 Human)

Use

Donor template

Tags

EGFP

Available Since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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MAPK9 gRNA (BRDN0001146002)

Plasmid

#76719

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK9

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK9 (MAPK9 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TGFBR2 gRNA (BRDN0001149310)

Plasmid

#77469

Purpose

3rd generation lentiviral gRNA plasmid targeting human TGFBR2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TGFBR2 (TGFBR2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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EPHB2 gRNA (BRDN0001146273)

Plasmid

#77545

Purpose

3rd generation lentiviral gRNA plasmid targeting human EPHB2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

EPHB2 (EPHB2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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AURKA gRNA (BRDN0001148015)

Plasmid

#77726

Purpose

3rd generation lentiviral gRNA plasmid targeting human AURKA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

AURKA (AURKA Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pOPINF Ta-sro1 WWE-PARP

Plasmid

#192550

Purpose

E. coli expression vector for Triticum aestivum sro1 WWE-PARP domains (amino acids 2-434)

Depositor

Lennart Wirthmueller

Article

Vogt et al Plant Cell. 2022 Aug 18. pii: 6671231. d

Insert

SIMILAR TO RCD ONE 1 (SRO1) WWE-PARP domains, cultivar Shanrong No. 3 (SR3)

Tags

His6, 3C protease cleavage site

Expression

Bacterial, Insect, and Mamm…

Promoter

T7 promoter

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223-GOLPH3

Plasmid

#21688

Depositor

Lynda Chin

Article

Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

Insert

Golgi Phosphoprotein 3 (GOLPH3 Human)

Use

Gateway entry clone

Mutation

last nucleotide of stop codon removed for c-termi…

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only
GCK gRNA (BRDN0001144982)

Plasmid

#77581

Purpose

3rd generation lentiviral gRNA plasmid targeting human GCK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

GCK (GCK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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SIN-cPPT-H1-sgHtt1-7SK-sgCas9-PGK-mCherry-WPRE

Plasmid

#87912

Purpose

Kamicas9 lentiviral vector

Depositor

Nicole Déglon

Article

Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

Insert

shHTT1, sgCas9, cherry

Use

Lentiviral

Promoter

H1, 7SK, PGK

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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LUC7L

Plasmid

#155713

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

LUC7L (LUC7L Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 18, 2022

Availability

Academic Institutions and Nonprofits only

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LYN gRNA (BRDN0001145823)

Plasmid

#77028

Purpose

3rd generation lentiviral gRNA plasmid targeting human LYN

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

LYN (LYN Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCDB181

Plasmid

#80692

Purpose

for secretion of N-terminal OsmY, deca-histidine, SUMO tagged gEHEE_06

Depositor

David Baker

Article

Bhardwaj et al Nature. 2016 Sep 14. doi: 10.1038/nature

Insert

gEHEE_06

Tags

OsmY-10His-Smt3

Expression

Bacterial

Promoter

T7

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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CLK2 gRNA (BRDN0001148263)

Plasmid

#77604

Purpose

3rd generation lentiviral gRNA plasmid targeting human CLK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CLK2 (CLK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CLK2 gRNA (BRDN0001145900)

Plasmid

#77607

Purpose

3rd generation lentiviral gRNA plasmid targeting human CLK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CLK2 (CLK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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