We narrowed to 9,085 results for: mel

1. pcDNAintron-EBOV-GP

   Plasmid

   #215692

   Purpose

   Produces the Ebola virus glycoprotein

   Depositor

   Melinda Brindley

   Article

   Acciani et al J Virol. 2021 Sep 27;95(20):e0116521. do

   Insert

   EBOV-GP
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pUDP293

   Plasmid

   #204228

   Purpose

   Episomal yeast plasmid expressing the Ercas12a and ErCas12a gRNA

   Depositor

   Jean-Marc Daran

   Article

   Bennis et al FEMS Yeast Res. 2023 Jan 4;23:foad043. d

   Inserts

   Eubacterium rectale cas12a (Ercas12a)
   BsaI_GFP drop-out_BsaI
   

   Use

   Synthetic Biology

   Tags

   NLS

   Mutation

   codon optimized for S. cerevisiae

   Promoter

   Saccharomyces cerevisiae PGK1 and Saccharomyces c…

   Available Since

   Sept. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNAintron-LASV-GPC

   Plasmid

   #215690

   Purpose

   Produces the Lassa virus glycoprotein

   Depositor

   Melinda Brindley

   Article

   Acciani et al J Virol. 2017 Aug 24;91(18):e00574-17. d

   Insert

   LASV GP
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pGEMHE-H2B-mEGFP

   Plasmid

   #105528

   Purpose

   T7 promotor drives in vitro transcription of mEGFP-tagged Histon 2B mRNA

   Depositor

   Melina Schuh

   Article

   Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

   Insert

   H2B (H2BC11 Human)
   

   Use

   In vitro transcription

   Tags

   mEGFP

   Promoter

   T7

   Available Since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. GFP-HuR

   Plasmid

   #121162

   Purpose

   Mammalian expression of GFP tagged HuR

   Depositor

   Christine Mayr

   Article

   Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

   Insert

   HuR (ELAVL1 )
   

   Expression

   Mammalian

   Available Since

   Feb. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5-FRT-TetO-U2B''-fSNAPc

   Plasmid

   #106988

   Purpose

   open reading frame of U2B'' inserted into pcDNA5-FRT-TetO-fSNAPc using the KpnI and NotI restriction sites

   Depositor

   Melissa Moore

   Article

   Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

   Insert

   UB2" (SNRPB2 Human)
   

   Tags

   fSNAP

   Available Since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-gfap(Intron1/5'/Exon1-zebrafish)

   Plasmid

   #39761

   Depositor

   Pamela Raymond

   Article

   Bernardos et al Gene Expr Patterns. 2006 Oct;6(8):1007-1

   Insert

   zebrafish gfap promoter- EGFP (gfap Zebrafish)
   

   Use

   Zebrafish expression

   Promoter

   gfap

   Available Since

   Aug. 21, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5-FRT-TetO-U1-70K-fSNAPc

   Plasmid

   #106987

   Purpose

   open reading frame of U1-70K inserted into pcDNA5-FRT-TetO-fSNAPc using the KpnI and NotI restriction sites

   Depositor

   Melissa Moore

   Article

   Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

   Insert

   U1-70K (SNRNP70 Human)
   

   Tags

   fSNAP

   Available Since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. 7MEQ

   Plasmid

   #176412

   Purpose

   Baculovirus expression for structure determination. May not contain entire coding region of gene.

   Depositor

   Cheryl Arrowsmith

   Insert

   TMPRSS2 – transmembrane serine protease 2 (TMPRSS2 Human)
   

   Use

   Baculovirus expresssion

   Tags

   8 x His Tag and Honeybee melittin signal

   Mutation

   etmprss2_S251D/R252D/Q253D/S254D/R255K

   Promoter

   Polyhedrin

   Available Since

   Oct. 21, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pHsp70-Cas9

    Plasmid

    #45945

    Purpose

    The codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter used in Gratz, et al. (2013). Plasmid is low copy number.

    Depositor

    Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger

    Article

    Gratz et al Genetics. 2013 May 24.

    Insert

    Codon optimized Cas9
    

    Use

    CRISPR

    Tags

    3x FLAG

    Mutation

    Silent change from C to A at bp 834 of insert

    Available Since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBABE.puro/TO/Flag/tGFP-HuR.wt

    Plasmid

    #110349

    Purpose

    Doxycycline inducible mammalian retroviral expression of HuR (wild type) with FLAG and turboGFP tags

    Depositor

    Sandra Martha Gomes Dias

    Insert

    ELAV like RNA binding protein 1 (ELAVL1 Human)
    

    Use

    Retroviral

    Tags

    FLAG and tGFP

    Expression

    Mammalian

    Promoter

    gag

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti Puro HA-Ubiquitin-K48

    Plasmid

    #236290

    Purpose

    Lentiviral vector expressing HA-tagged ubiquitin K48 mutant

    Depositor

    Melina Fan, Addgene Research Program

    Insert

    Ubiquitin K48 (UBB Human)
    

    Use

    Lentiviral

    Tags

    HA

    Mutation

    Single ubiquitin coding sequence containing K48 m…

    Promoter

    CMV

    Available Since

    April 10, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pLenti Puro HA-Ubiquitin-K63

    Plasmid

    #236291

    Purpose

    Lentiviral vector expressing HA-tagged ubiquitin K63 mutant

    Depositor

    Melina Fan, Addgene Research Program

    Insert

    Ubiquitin K63 (UBB Human)
    

    Use

    Lentiviral

    Tags

    HA

    Mutation

    Single ubiquitin coding sequence containing K63 m…

    Promoter

    CMV

    Available Since

    April 10, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pGEMHE-GST

    Plasmid

    #171486

    Purpose

    T7 promotor drives in vitro transcription of GST mRNA

    Depositor

    Melina Schuh

    Article

    Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

    Insert

    GST
    

    Use

    Pgehme

    Promoter

    T7

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV5-myc-Erk2

    Plasmid

    #39222

    Depositor

    Melanie Cobb

    Article

    Xu et al J Biol Chem. 2001 Jul 13;276(28):26509-1

    Insert

    Erk2 (Mapk1 Rat)
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

16. 1137=Hsp70Bb-Cas9[dsRed]

    Plasmid

    #149424

    Purpose

    The piggyBac plasmid harboring Hsp70Bb-Cas9-T2A-eGFP-p10 and Opie2-dsRed-SV10 (marker gene)

    Depositor

    Omar Akbari

    Article

    Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    eGFP

    Expression

    Insect

    Promoter

    Dmel Hsp70Bb

    Available Since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJK506

    Plasmid

    #155382

    Purpose

    dCas12a (D917A) + PA4-mVenus. (removed crRNAs)

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas12a (F. novicida)
    PA4-mVenus
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    D917A (nuclease-deactivating)

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. NpT7-5-Erk2

    Plasmid

    #39230

    Depositor

    Melanie Cobb

    Article

    Robbins et al J Biol Chem. 1993 Mar 5;268(7):5097-106.

    Insert

    Erk2 (Mapk1 Rat)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pH6HTC_hsHUR-Halo

    Plasmid

    #175303

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    HUR (ELAVL1 Human)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Available Since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA5/FRT/TO/Flag-tGFP-HuR.wt

    Plasmid

    #110376

    Purpose

    Mammalian expression of turboGFP-HuR (wild type) with FLAG tag, Flp-In T-Rex system

    Depositor

    Sandra Martha Gomes Dias

    Insert

    ELAV like RNA binding protein 1 (ELAVL1 Human)
    

    Tags

    FLAG and tGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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