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SlugCas9-HF

Plasmid

#163796

Purpose

Expresses highly specific SlugCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

Type

Empty backbone

Use

AAV and CRISPR

Expression

Mammalian

Mutation

SlugCas9 (R247A, N415A, T421A, R656A)

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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AAVS1-Neo-TRE-CMV-Cre-rtTA

Plasmid

#165457

Purpose

Tetracycline or doxycycline inducible CRE expression from AAVS1 locus

Depositor

Madeline Lancaster

Article

Benito-Kwiecinski et al Cell. 2021 Apr 15;184(8):2084-2102.e19.

Insert

CRE

Expression

Mammalian

Promoter

Tight TRE promoter

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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HyperADAR control

Plasmid

#166969

Purpose

Express the Hyperactive catalytic domain of Drosophila ADAR and linker at the 5' end of this domain, in the MSCV-IRES-GFP

Depositor

Michael Kharas

Article

Nguyen et al Nat Commun. 2020 Apr 24;11(1):2026. doi:

Insert

linker-hyperADAR

Use

Retroviral

Tags

linker-HyperADAR

Expression

Mammalian

Promoter

MSCV

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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ORF3a-EGFP

Plasmid

#165121

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

ORF3a (ORF3a codon optimized SARS-COV-2)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX/PD-L1 WT

Plasmid

#121466

Purpose

Expression of GST-human PD-L1 in E. Coli

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Insert

CD274 (CD274 Human)

Tags

GST

Expression

Bacterial

Promoter

Tac

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-PTDSS1-IRES-Puromycin

Plasmid

#134667

Purpose

Expresses EGFP-tagged Mitochondria-associated ER membrane marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

PTDSS1 (PTDSS1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast POLE1

Plasmid

#160802

Purpose

Express POLE1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

POLE1 (POLE Human)

Use

Retroviral

Tags

3xFLAG

Mutation

shPOLE_1 and shPOLE2 resistant

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pUC57-Curlcake3

Plasmid

#139342

Purpose

Expresses Curlcake3 (CC3) in E.coli

Depositor

Eva Maria Novoa

Article

Liu et al Nat Commun. 2019 Sep 9;10(1):4079. doi:

Insert

Curlcake3 with T7 promoter

Expression

Bacterial

Promoter

T7

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pX330-gRNA

Plasmid

#158973

Purpose

A human codon-optimized SpCas9 and chimeric guide RNA (gRNA3) expression plasmid.

Depositor

Charles P. Lai

Article

Chien et al Nucleic Acids Res. 2020 Aug 14. pii: 589

Insert

gRNA3

Expression

Mammalian

Promoter

U6

Available Since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pBWB162

Plasmid

#140634

Purpose

pBAV1k-lacI-Trc-mCherry base, lacI repression vector for ADP1

Depositor

Keith Tyo

Article

Biggs et al Nucleic Acids Res. 2020 Apr 4. pii: 5815

Insert

mCherry

Expression

Bacterial

Available Since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-His:dCas9:nls:10P3

Plasmid

#135982

Purpose

Expresses dCas9 fused to 10 copies of the P3 coiled coil in mammalian cells

Depositor

Roman Jerala

Article

Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

Insert

dCas9 fused to 10 copies of the P3 coiled-coil

Use

CRISPR

Tags

His

Expression

Mammalian

Mutation

D10A; H840A in S.pyogenes Cas9

Promoter

CMV

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-mCherry-anti-mouse-IgG(kappa)-nanobody

Plasmid

#138130

Purpose

Expresses GST-tagged mCherry-anti-mouse-IgG(kappa)-nanobody in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

Insert

mCherry-anti-mouse-IgG(kappa)-nanobody

Tags

GST

Expression

Bacterial

Available Since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-GRAB_Ado1.0mut

Plasmid

#153287

Purpose

Express the adenosine-insensitive control sensor GRAB_Ado1.0mut in neurons

Depositor

Yulong Li

Article

Peng et al Science. 2020 Sep 4;369(6508). pii: 369/

Insert

Adenosine-insensitive sensor GRAB_Ado1.0mut (control sensor)

Use

AAV

Promoter

hSyn

Available Since

July 7, 2020

Availability

Academic Institutions and Nonprofits only

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pMP71-hPDL-1

Plasmid

#118631

Purpose

human PDL-1 coding sequence subcloned into pMP71 retroviral plasmid

Depositor

Sébastien Walchli

Article

Josefsson et al Cancer Immunol Res. 2019 Mar;7(3):355-36

Insert

hPDL-1 (CD274 Human)

Expression

Mammalian

Promoter

LTR

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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epiCRISPR

Plasmid

#135960

Purpose

Expresses SpCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Xie et al Sci Rep. 2017 May 24;7(1):2320. doi: 10.

Type

Empty backbone

Expression

Mammalian

Available Since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Sox2-CreERT2

Plasmid

#135618

Purpose

Donor vector for genomic targeting of a P2A-CreERT2 cassette to the 3' end of the mouse Sox2 locus

Depositor

Lin He

Article

Chen et al Cell Rep. 2019 Jun 25;27(13):3780-3789.e

Insert

P2A-CreERT2 flanked by Sox2 homology arms (Sox2 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

P2A-CreERT2

Available Since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-PI4KB

Plasmid

#139310

Purpose

Recruitable PI4KB

Depositor

Gerry Hammond

Article

Zewe et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

mCherry: FKBP1A(3-108): GGSA]4GG:PI4KB (PI4KB Human)

Expression

Mammalian

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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EMTB-mScarletI

Plasmid

#137801

Purpose

Visualization of microtubules

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

Ensconsin microtubule binding domain

Expression

Mammalian

Promoter

CMV

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-VAMP5-IRES-Puromycin

Plasmid

#134662

Purpose

Expresses EGFP-tagged plasma membrane marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

vesicle-associated membrane protein 5 (VAMP5 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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amilCP_Orange chromoprotein

Plasmid

#117850

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses amilCP_Orange chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, amilCP_Orange

Use

Synthetic Biology; Escherichia coli

Mutation

64V,65G, BioBrick sites removed

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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