We narrowed to 59,379 results for: %s

1. pX458-MMERVK9C-del-gRNA2

   Plasmid

   #185005

   Purpose

   expression of gRNA and Cas9 from S. pyogenes with 2A-EGFP

   Depositor

   Denes Hnisz

   Article

   Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

   Insert

   MMERVK9C-del-gRNA2
   

   Use

   CRISPR

   Available Since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX458-IAP-del-gRNA2

   Plasmid

   #185003

   Purpose

   expression of gRNA and Cas9 from S. pyogenes with 2A-EGFP

   Depositor

   Denes Hnisz

   Article

   Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

   Insert

   IAP-del-gRNA2 (Akp3 Mouse)
   

   Use

   CRISPR

   Available Since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDON223 Spike L241-L242-A243-

   Plasmid

   #180070

   Purpose

   Sars-CoV-2 protein mutation

   Depositor

   Markku Varjosalo

   Article

   Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

   Insert

   SARS-CoV-2 Spike L241-L242-A243- (S SARS-CoV-2)
   

   Tags

   MAC-tag

   Expression

   Mammalian

   Mutation

   Spike L241-L242-A243-

   Available Since

   Jan. 13, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pIFU002

   Plasmid

   #174299

   Purpose

   Encodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) with the N501Y mutation and can be combined with pJS697 (Addgene plasmid # 168778) through homologous recombination

   Depositor

   Timothy Whitehead

   Article

   Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

   Insert

   Spike RBD N343Q, N501Y (S Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   HA and cMyc

   Expression

   Yeast

   Mutation

   N343Q, N501Y

   Available Since

   Sept. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIFU001

   Plasmid

   #174298

   Purpose

   Encodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) with the E484K mutation and can be combined with pJS697 (Addgene plasmid # 168778) through homologous recombination

   Depositor

   Timothy Whitehead

   Article

   Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

   Insert

   Spike RBD N343Q, E484K (S Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   HA and cMyc

   Expression

   Yeast

   Mutation

   N343Q, E484K

   Available Since

   Sept. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. R619-M96-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(318-529)-3C-His8-SBP

   Plasmid

   #166020

   Purpose

   mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD) optimized for serology

   Depositor

   Dominic Esposito

   Article

   Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

   Insert

   SARS-CoV-2 RBD (S )
   

   Tags

   3C-His8-SBP

   Expression

   Mammalian

   Promoter

   CMV51

   Available Since

   March 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. Met1 diUb (1-152, R42W)

   Plasmid

   #110767

   Purpose

   Bacterial expression of Met1 diUb

   Depositor

   David Komander

   Article

   Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

   Insert

   UBB (UBB Human)
   

   Tags

   N-His6-3C

   Expression

   Bacterial

   Mutation

   R42W

   Promoter

   T7

   Available Since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. tdEos-EB3-7

   Plasmid

   #57610

   Purpose

   Localization: MT End Binding Protein, Excitation: 505 / 569, Emission: 516 / 581

   Depositor

   Michael Davidson

   Article

   Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

   Insert

   EB3 (MAPRE3 Human)
   

   Tags

   tdEos

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 16, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

9. pYJA5

   Plasmid

   #217778

   Purpose

   The empty vector for quadruple sgRNA cloning

   Depositor

   Adriano Aguzzi

   Article

   Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038

   Type

   Empty backbone

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Tags

   Puromycin resistance gene, T2A, and TagBFP

   Expression

   Mammalian

   Promoter

   PGK, CMV, U6

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-TRE3G-YAP1-5SA-CMV-mCherry-T2A-Puro

    Plasmid

    #176851

    Purpose

    lentiviral vector for doxycycline-inducible YAP1 5SA constitutively active expression

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    hYAP1-5SA (YAP1 Human)
    

    Use

    Lentiviral

    Mutation

    S61A, S109A, S127A, S164A, S381A (phosphorylation…

    Available Since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLEX-307_LgBiT

    Plasmid

    #191211

    Purpose

    Lentiviral-mediated stable expression of LgBiT for slit nanoluciferase complementation assays in mammalian cells

    Depositor

    Mohan Babu

    Article

    Broderick et al Nat Commun. 2025 Jul 1;16(1):5784. doi:

    Insert

    LgBiT (large bit)
    

    Use

    Lentiviral and Luciferase

    Mutation

    AB030246.1 (mutated in PMID 26569370)

    Available Since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pmEos2-N1 PH-PLCd (wild-type) (PH-PLCd-mEos2)

    Plasmid

    #162877

    Purpose

    Expression in mammalian cells of Plekstrin-homology domain of Phospholipase C delta tagged with mEos2 to perform sptPALM

    Depositor

    Frederic Meunier

    Article

    Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

    Insert

    pleckstrin homology domain of PLCδ1 (phospholipase C-δ1) (PLCD1 Human)
    

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    Insert consists of AA1-170

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV-hSyn-ASAP4e-Kv-WPRE

    Plasmid

    #201031

    Purpose

    Expresses somatically enriched ASAP4e in neurons, can be used to package AAV

    Depositor

    Michael Lin

    Article

    Evans et al Nat Methods. 2023 Jul;20(7):1104-1113. d

    Insert

    ASAP4e-Kv
    

    Use

    AAV

    Available Since

    July 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJL1-sfGFP

    Plasmid

    #102634

    Purpose

    In vitro expression of sfGFP from the T7 promoter

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

    Insert

    sfGFP
    

    Tags

    strep tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-pro-Il1b-C-Flag

    Plasmid

    #75131

    Purpose

    Expresses C-terminal flag-tagged pro-IL-1β in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    interleukin 1 beta (Il1b Mouse)
    

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    (please see depositor comments below)

    Promoter

    CMV

    Available Since

    Sept. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. P3-Lenti-dCas9-Tet1-GFP

    Plasmid

    #190729

    Purpose

    Lentiviral construct to express spdCas9-Tet1-GFP.

    Depositor

    Maja Jagodic

    Article

    Pahlevan Kakhki et al Nat Commun. 2024 Jul 30;15(1):6419. doi:

    Insert

    dCas9-tet1
    

    Use

    CRISPR

    Tags

    SV40 NLS and T2A-EGFP

    Expression

    Mammalian

    Mutation

    Changed Pro434 codon from CCG to CCC and Gly468 f…

    Promoter

    UbC

    Available Since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMVtrunc F-tractinN9-39-msfGFPΔN7

    Plasmid

    #231552

    Purpose

    Mammalian expression of C-terminally truncated F-tractin fused to N-terminally truncated monomeric sfGFP, for actin filament organization measurements using fluorescence polarization microscopy

    Depositor

    Manos Mavrakis

    Article

    Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

    Insert

    F-tractin (Itpka Rat)
    

    Tags

    msfGFP

    Expression

    Mammalian

    Promoter

    CMVtrunc

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-N-Flag-NLRP3 711-1034

    Plasmid

    #75141

    Purpose

    Expresses N-terminal Flag-tagged amino acids 711-1034 of NLRP3 (the LRR domain) in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    NLR family, pyrin domain containing 3 (Nlrp3 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    fragment encoding amino acids 711-1034

    Promoter

    CMV

    Available Since

    May 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-N-Flag-NLRP3 1-90

    Plasmid

    #75137

    Purpose

    Expresses N-terminal Flag-tagged amino acids 1-90 of NLRP3 (the pyrin domain) in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    NLR family, pyrin domain containing 3 (Nlrp3 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    fragment encoding amino acids 1-90

    Promoter

    CMV

    Available Since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AAV-EF1a-FLEx-ASAP4e-Kv-WPRE

    Plasmid

    #201033

    Purpose

    Expresses somatically enriched ASAP4e from the EF-1alpha promoter in a cre-dependent manner

    Depositor

    Michael Lin

    Article

    Evans et al Nat Methods. 2023 Jul;20(7):1104-1113. d

    Insert

    ASAP4b-Kv
    

    Use

    AAV

    Available Since

    July 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge