We narrowed to 4,709 results for: golden gate
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Plasmid#226962PurposeCBh-SaCas9-2A-GFP, and hU6-sgRNA (Sa) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.DepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable SinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only
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pSB123 - pL0_rLUC-I (CDS1)
Plasmid#123181PurposeGolden Gate (MoClo; CDS1) compatible luciferase gene from Renilla reniformis with an intron for transient and stable expression in plantsDepositorInsertluciferase gene from Renilla reniformis with an plant intron and codon optimized for plants
UseLuciferase; Part for plant expressionExpressionPlantMutationNo BpiI and BsaI sites; codon-optimised for plantsAvailable SinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28:LanIII
Plasmid#225075PurposeExpresses precursor peptide and lanthipeptide synthetase from LanIII (FAST-RiPPs) in E. coliDepositorInsertLanA (LanIII)
TagsHis6ExpressionBacterialMutationWTPromoterT7Available SinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28:LanIII-43
Plasmid#225077PurposeExpresses precursor peptide and lanthipeptide synthetase from LanIII-43 (FAST-RiPPs) in E. coliDepositorInsertLanA (LanIII-43)
TagsHis6ExpressionBacterialMutationWTPromoterT7Available SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28:LanII
Plasmid#225066PurposeExpresses precursor peptide and lanthipeptide synthetase from LanII (FAST-RiPPs) in E. coliDepositorInsertLanA (LanII)
TagsHis6ExpressionBacterialMutationWTPromoterT7Available SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pICSL11060
Plasmid#68264PurposeLevel 1 Golden Gate Cassette: Cas9 expression cassette for plants (exemplified in Brassica oleracea)DepositorInsertPromoter/5UTR: CsVMV+ CDS:SpCas9 + 3UTR/terminator:35s
UseSynthetic BiologyExpressionPlantAvailable SinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCKC775
Plasmid#226626PurposepiggyBAC 7xTCF-LEF hCas9-T2A-A/T-biased2DepositorInsertsUseSynthetic BiologyExpressionMammalianPromoter7xTCF-LEF and EF1aAvailable SinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCKC764
Plasmid#226625PurposepiggyBAC pCAG-hCas9-T2A-Unbiased3DepositorInsertsCAG promoter, SpCas9, T2A, Unbiased3 variant of TdT (DNTT Chicken, Human, S. pyogenes)
EF1a promoter, blasticidin resistance
UseSynthetic BiologyExpressionMammalianPromoterCMV and EF1aAvailable SinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCKC776
Plasmid#226627PurposepiggyBAC 4xHRE YB_TATA hCas9-T2A-G/C-biased1DepositorInserts4x HRE_YB TATA, SpCas9, oxygen-dependent degron, T2A, G/C-biased1 variant of TdT (DNTT S. pyogenes, Human)
EF1a promoter, mCherry
UseSynthetic BiologyTagsoxygen dependent degron (ODD)ExpressionMammalianPromoter4xHRE_YB TATA and EF1aAvailable SinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-ITR-EcYtR-mCherry
Plasmid#217362PurposeExpresses E. coli tyrosine tRNA and a wild-type mCherry reporter; can be packaged into AAVDepositorInsertE. coli tyrosine tRNA
UseAAVExpressionMammalianPromoterU6Available SinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only