We narrowed to 5,620 results for: Feng Zhang

1. AXBEv2

   Plasmid

   #204606

   Purpose

   Mammalian expression of evolved adenine transversion base editor AXBEv2

   Depositor

   Dali Li

   Article

   Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103

   Insert

   bpNLS-TadA-8e-nSpCas9-mAAG (R165E/Y179F)-bpNLS-P2A-GFP
   

   Use

   CRISPR

   Tags

   6*His

   Expression

   Mammalian

   Mutation

   mAAG (R165E/Y179F); S. pyogenes Cas9 (D10A)

   Promoter

   Available since

   Aug. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. ACBE

   Plasmid

   #204607

   Purpose

   Mammalian expression of adenine-to-cytosine base editor ACBE

   Depositor

   Dali Li

   Article

   Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103

   Insert

   bpNLS-N terminal of nSpCas9-linker-mAAG (R165E/Y179F)-TadA-8e-linker-C terminal of nSpCas9-bpNLS-P2A-GFP
   

   Use

   CRISPR

   Tags

   6*His

   Expression

   Mammalian

   Mutation

   mAAG (R165E/Y179F); S. pyogenes Cas9 (D10A)

   Promoter

   Available since

   Aug. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. AXBE

   Plasmid

   #204605

   Purpose

   Mammalian expression of adenine transversion base editor AXBE

   Depositor

   Dali Li

   Article

   Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103

   Insert

   bpNLS-TadA-8e-nSpCas9-mAAG-bpNLS-P2A-GFP
   

   Use

   CRISPR

   Tags

   6*His

   Expression

   Mammalian

   Mutation

   S. pyogenes Cas9 (D10A)

   Promoter

   Available since

   Aug. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBoBi-hLAMP2-C-PEN2

   Plasmid

   #183646

   Purpose

   Lentiviral expression of a lysosome-residential PEN2.

   Depositor

   Sheng-cai Lin

   Article

   Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

   Insert

   PEN2 and LAMP2 (PSENEN, LAMP2 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   WT

   Promoter

   CMV

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.3-hAP1 1-419

   Plasmid

   #183644

   Purpose

   To (ectopically) express a mutant of ATP6AP1 lacking the interacting domain with PEN2; mammalian expression

   Depositor

   Sheng-cai Lin

   Article

   Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

   Insert

   ATP6AP1 (ATP6AP1 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   truncated ATP6AP1 mutant; contains only amino aci…

   Promoter

   CMV

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.3-hAP1 420-440

   Plasmid

   #183643

   Purpose

   To (ectopically) express a mutant of ATP6AP1 lacking the interacting domain with PEN2; mammalian expression

   Depositor

   Sheng-cai Lin

   Article

   Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

   Insert

   ATP6AP1 (ATP6AP1 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   truncated ATP6AP1 mutant; contains only the trans…

   Promoter

   CMV

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBoBi-hAP1 Δ420-440

   Plasmid

   #183642

   Purpose

   To (stably) express a mutant of ATP6AP1 lacking the interacting domain with PEN2; lentiviral expression

   Depositor

   Sheng-cai Lin

   Article

   Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

   Insert

   ATP6AP1 (ATP6AP1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   truncated ATP6AP1 mutant lacking the transmembran…

   Promoter

   CMV

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLentiCRISPR_mCherry

   Plasmid

   #229078

   Purpose

   Small lentiviral backbone for CRISPR guide libraries, improved titre for hard to transduce cells

   Depositor

   Bernhard Schmierer

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1s

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. PH-Btk-mRuby2

   Plasmid

   #124657

   Purpose

   PI(3,4,5)P3 probe. PH domain of BTK fused with mRuby2

   Depositor

   Cheng-han Yu

   Article

   Zhang et al Mol Biol Cell. 2019 Mar 1;30(5):622-635.

   Insert

   BTK (BTK Human)
   

   Use

   Tags

   mRuby2

   Expression

   Mammalian

   Mutation

   contains only the PH domain of Bruton tyrosine ki…

   Promoter

   Available since

   May 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAdEasy-hALDOA

    Plasmid

    #210812

    Purpose

    To package adenovirus expressing wild-type aldolaseA

    Depositor

    Sheng-cai Lin

    Article

    Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

    Insert

    ALDOA (ALDOA Human)
    

    Use

    Adenoviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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