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pEM7_CL7_Bac_2NLS-Flex-Cas12a (LbCas12a)-4NLS

Plasmid

#244842

Purpose

Bacterial expression of Flex-Cas12a LbCas12a with 2xSV40 NLS at N-terminus and 4xSV40 at C-terminus

Depositor

Jennifer Doudna

Article

Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

Flex-Cas12a

Use

CRISPR

Tags

10xHis-CL7-2xSV40 NLS and 4xSV40 NLS

Expression

Bacterial

Mutation

G146R/R182V/D535G/S551F/D665N/E795Q

Promoter

T7

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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TOPO-SV40

Plasmid

#226453

Purpose

For subcloning of SV40 promoter or for assays using M13 phage

Depositor

Glen Borchert

Article

DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

Insert

SV40

Use

Cloning vector

Mutation

No

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pRSF-G1(7CNW)RS

Plasmid

#222874

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of 7-Cyano-L-Tryptophan (7-CN-Trp) into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Article

Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

Insert

tRNA synthetase

Expression

Bacterial

Promoter

T7

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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ECFP-FRB-Sec61b

Plasmid

#210290

Purpose

nucleus OM marker and "anchor" for FKBP-FRB CID

Depositor

Takanari Inoue

Article

Nakamura et al Cell Rep. 2023 Sep 2:113089. doi: 10.101

Insert

ECFP-FRB-Sec61b

Tags

ECFP and FRB

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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Tom20-EYFP-miLID

Plasmid

#210291

Purpose

mitochondria marker and "anchor" for optogenetics

Depositor

Takanari Inoue

Article

Nakamura et al Cell Rep. 2023 Sep 2:113089. doi: 10.101

Insert

Tom20-EYFP-miLID

Tags

EYFP and iLID

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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mActA-mSspB-mCherry

Plasmid

#210294

Purpose

ActuAtor for iLID optogenetics

Depositor

Takanari Inoue

Article

Nakamura et al Cell Rep. 2023 Sep 2:113089. doi: 10.101

Insert

mActA-,SspB-mCherry

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pEMS2075

Plasmid

#158098

Purpose

High Copy number plasmid containing smCBA insert.

Depositor

Elizabeth Simpson

Article

de Moura Gomes et al Sci Rep. 2024 Nov 15;14(1):28194. doi: 1

Insert

smCBA

Promoter

smCBA

Available Since

Nov. 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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PGK-LCB3_v1.2-NotchGal4VP64-T2A-mCherry (pZYW047)

Plasmid

#194230

Purpose

Lentiviral expression vector expressing LCB3 SynNotch and mCherry transduction marker

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv 2021.04.20.440678

Insert

PGK-LCB3-SynNotch-T2A-mCherry

Use

Lentiviral

Mutation

E45D mutation in LCB3

Promoter

PGK

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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EF1a-LCB3_v1.2-PDGFR-T2A-mCherry (pZYW049)

Plasmid

#194232

Purpose

Lentiviral expression vector expressing LCB3 on the cell surface attached to PDGFR's transmembrane domain and mCherry transduction marker

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv 2021.04.20.440678

Insert

EF1a-LCB3-PDGFR TMD-T2A-mCherry

Use

Lentiviral

Promoter

EF1a

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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L2111-FKBP-GPA-20-11S

Plasmid

#222882

Purpose

Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 11S.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FKBP-GPA-20GS-NanoLuc11S

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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