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pPD032 Reporter Template v1 (Mid Spaced)

Plasmid

#138753

Purpose

Template for COMET promoters, expresses EYFP reporter upon promoter activation.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pPD033 Reporter Template v1 (Far Spaced)

Plasmid

#138754

Purpose

Template for COMET promoters, expresses EYFP reporter upon promoter activation.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pPD529 VP16-ZF36

Plasmid

#138758

Purpose

Constitutive expression of VP16-ZF36 under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VP16-ZF36

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pHIE059 ZF43x1 mKate2 EF1a EBFP2-P2A-BlastR

Plasmid

#138740

Purpose

Integration Vector, assembled with pHIE043 (TUPV1), pJM451 (TUPV2), pPD612

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x1

Use

Synthetic Biology

Expression

Mammalian

Available Since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pHIE060 ZF43x3-C mKate2 EF1a EBFP2-P2A-BlastR

Plasmid

#138741

Purpose

Integration Vector, assembled with pHIE044 (TUPV1), pJM451 (TUPV2), pPD612

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x3-Compact

Use

Synthetic Biology

Expression

Mammalian

Available Since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pHIE063 ZF43x3-S mKate2 EF1a EBFP2-P2A-BlastR

Plasmid

#138744

Purpose

Integration Vector, assembled with pHIE047 (TUPV1), pJM451 (TUPV2), pPD612

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x3-Spaced

Use

Synthetic Biology

Expression

Mammalian

Available Since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHIE064 ZF43x6-S mKate2 EF1a EBFP2-P2A-BlastR

Plasmid

#138745

Purpose

Integration Vector, assembled with pHIE048 (TUPV1), pJM451 (TUPV2), pPD612

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x6-Spaced

Use

Synthetic Biology

Expression

Mammalian

Available Since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHIE062 ZF43x12-C mKate2 EF1a EBFP2-P2A-BlastR

Plasmid

#138743

Purpose

Integration Vector, assembled with pHIE046 (TUPV1), pJM451 (TUPV2), pPD612

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x12-Compact

Use

Synthetic Biology

Expression

Mammalian

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pJM466 EF1a VP16-ZF43 (TUPV2)

Plasmid

#138730

Purpose

mMoClo TUPV, with EF1alpha promoter and VP16-ZFa in the MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VP16-ZF43

Use

Synthetic Biology

Expression

Mammalian

Promoter

hEF1alpha

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pHIE048 ZF43x6-S mKate2 (TUPV1)

Plasmid

#138728

Purpose

mMoClo TUPV, with ZF43x6-S promoter and mKate2 in the MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x6-Spaced

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x6-Spaced

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHIE047 ZF43x3-S mKate2 (TUPV1)

Plasmid

#138727

Purpose

mMoClo TUPV, with ZF43x3-S promoter and mKate2 in the MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x3-Spaced

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x3-Spaced

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pHIE045 ZF43x6-C mKate2 (TUPV1)

Plasmid

#138725

Purpose

mMoClo TUPV, with ZF43x6-C promoter and mKate2 in the MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x6-Compact

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x6-Compact

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pHIE044 ZF43x3-C mKate2 (TUPV1)

Plasmid

#138724

Purpose

mMoClo TUPV, with ZF43x3-C promoter and mKate2 in the MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x3-Compact

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x3-Compact

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pHIE049 ZF43x12-S mKate2 (TUPV1)

Plasmid

#138729

Purpose

mMoClo TUPV, with ZF43x12-S promoter and mKate2 in the MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x12-Spaced

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x12-Spaced

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pmTgfa UTR

Plasmid

#31256

Depositor

David Lee

Article

Kornblum et al J Comp Neurol. 1997 Apr 7. 380(2):243-61

Insert

Tgfa (Tgfa Mouse)

Use

Cdna for in situ hybridization

Mutation

Exon 6 of Tgfa with 3'UTR

Available Since

Dec. 5, 2011

Availability

Academic Institutions and Nonprofits only
EV-A71 2A protease; aliases: Enterovirus EV71 2A protease, 6xHis-SUMO-TEV tag, self cleaving

Plasmid

#204810

Purpose

Bacterial expresison of Enterovirus A71 2A protease

Depositor

ASAP Discovery, Frank von Delft

Insert

A71 2A protease

Expression

Bacterial

Available Since

Dec. 18, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pLN426 (FuGW-G5p-mKate2)

Plasmid

#105183

Purpose

Module 3 - GAL4 synthetic promoter (with five binding sites) drives mKate2 expression

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

G5p-mKate2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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MERS Mpro - His6-SUMO with autoprocessing - MSAVMQ/SGLVKM---MGVVMQ/SGVRKVTYGTAHWL

Plasmid

#204790

Purpose

Bacterial expression of MERS Mpro

Depositor

ASAP Discovery, Frank von Delft

Insert

MERS Mpro

Expression

Bacterial

Mutation

MSAVMQ/SGLVKM

Available Since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MERS Mpro - His6-SUMO with autoprocessing - MSAVLQ/SGLVKM---MGVVMQ/SGVRKVTYGTAHWL

Plasmid

#204814

Purpose

Bacterial expression of MERS Mpro

Depositor

ASAP Discovery, Frank von Delft

Insert

MERS Mpro (orf1ab MERS)

Expression

Bacterial

Mutation

MSAVLQ/SGLVKM

Available Since

Feb. 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
EV-D68 2A protease; 6xHis-MBP-TEV tag, low expression levels

Plasmid

#204803

Purpose

Bacterial expresison of Enterovirus D68 2A protease

Depositor

ASAP Discovery, Frank von Delft

Insert

D68 2A protease

Expression

Bacterial

Available Since

Dec. 11, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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