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pALOD4 non-binding mutant

Plasmid

#111027

Purpose

Expresses non-binding mutant version of p-ALOD4, has six inactivating mutations (G501A, T502A, T503A, L504A, Y505A, and P506A)

Depositor

Arun Radhakrishnan

Article

Gay et al Biophys J. 2015 Mar 24;108(6):1459-69. d

Insert

Domain 4 of Anthrolysin O

Use

Tags

His6 tag

Expression

Bacterial

Mutation

Mutations: S404C, C472A, G501A, T502A, T503A, L5…

Promoter

T7

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pLYS1-EMRE/RNAi resistant

Plasmid

#50056

Purpose

Expression of human EMRE in mammalian cells with silent mutation making it resistant to RNAi

Depositor

Vamsi Mootha

Article

Sancak et al Science. 2013 Nov 14.

Insert

EMRE-RNAi resistant (SMDT1 Human)

Use

Tags

Expression

Mammalian

Mutation

RNAi resistant, silent mutant

Promoter

Available since

Feb. 24, 2014

Availability

Academic Institutions and Nonprofits only

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Anti-Ankyrin-G [N106/36R-1]

Plasmid

#206697

Purpose

Mammalian Expression Plasmid of anti-Ankyrin-G (Human). Derived from hybridoma N106/36-1.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Ankyrin-G (Homo sapiens) recombinant Mouse monoclonal antibody (ANK3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pHDR_REC8-T2A-mGreenLantern-puroTK

Plasmid

#222521

Purpose

Homology directed repair template for knocking in mGreenLantern reporter to REC8.

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

mGreenLantern

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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Anti-GluN2A [N327/95R]

Plasmid

#177531

Purpose

Mammalian Expression Plasmid of anti-GluN2A/NR2A glutamate receptor (Rat). Derived from hybridoma N327/95.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-GluN2A/NR2A glutamate receptor (Rattus norvegicus) recombinant mouse monoclonal antibody (Grin2a Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR FL CPAP RNAi resistant

Plasmid

#46390

Depositor

Pierre Gonczy

Article

Kitagawa et al J Cell Sci. 2011 Nov 15;124(Pt 22):3884-

Insert

CPAP RNAi resistant mutant (CENPJ Human)

Use

Entry vector

Tags

Expression

Mutation

silent mutation that renders it resistant to siRN…

Promoter

Available since

July 12, 2013

Availability

Academic Institutions and Nonprofits only
K9L mutant of H3K9me3 biosensor

Plasmid

#120807

Purpose

FRET biosensor. Eliminating the H3 lysine 9 methylation site in H3K9me3 biosensor to abolish the detection capability

Depositor

Yingxiao Wang

Article

Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

Insert

Truncated YPet-HP1-EV linker-ECFP-mouse histone H3(K9L mutation)

Use

Tags

Expression

Mammalian

Mutation

lysine 9 is mutated to leucine 9 on histone H3

Promoter

CMV

Available since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-Kv3.1b K+ channel [N16B/8R]

Plasmid

#114511

Purpose

Mammalian Expression Plasmid of anti-Kv3.1b K+ channel (Rat). Derived from hybridoma N16B/8.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Kv3.1b K+ channel (Rattus norvegicus) recombinant mouse monoclonal antibody (Kcnc1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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Anti-Gephyrin [L106/83R]

Plasmid

#182100

Purpose

Mammalian Expression Plasmid of anti-Gephyrin (Human). Derived from hybridoma L106/83.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Gephyrin (Homo sapiens) recombinant mouse monoclonal antibody (GPHN Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-QKI-6 [N182/17R-2b]

Plasmid

#206661

Purpose

Mammalian Expression Plasmid of anti-QKI-6 (Human). Derived from hybridoma N182/17-2b.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-QKI-6 (Homo sapiens) recombinant Mouse monoclonal antibody (QKI Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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