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Plasmid#192664Purpose3rd generation lenti vector encoding dCas9-KRAB-MeCP2 with 2A Hygro resistance markerDepositorInsertdCas9-KRAB-MeCP2
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A, D839A, H840A and N863A in Cas9PromoterEF1aAvailable sinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mouse PKA-Calpha-mEGFP
Plasmid#45521DepositorInsertPKA catalytic subunit alpha tagged by mEGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-anti-TNFalpha(Ozoralizumab) PAGER(FL)
Plasmid#230024PurposeExpresses anti-TNFalpha PAGER(FL) in mammalian cellsDepositorInsertpAAV-MT3-Ozoralizumab-TEVcs-ALFA-hM4DxcpGFP
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-anti-TNFalpha(Ozoralizumab) PAGER(Gq)
Plasmid#230015PurposeExpresses anti-TNFalpha PAGER(Gq) in mammalian cellsDepositorInsertMT1-Ozoralizumab-TEVcs-ALFA-hM1D
UseAAVTagsExpressionMammalianMutationW442A on hM1DPromoterCMVAvailable sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-anti-TNFalpha(Ozoralizumab) PAGER(TF)
Plasmid#230001PurposeExpresses anti-TNFalpha PAGER(TF) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)DepositorInsertIL2SP-Aro6-Ozoralizumab-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
UseAAVTagsExpressionMammalianMutationV360A/R361A on KORDPromoterCMVAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSV human p85 alpha (HA tag)
Plasmid#11499DepositorInsertp85 alpha (PIK3R1 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJuly 19, 2006AvailabilityAcademic Institutions and Nonprofits only -
pDGB3 alpha2 AtUBQ10:LbCas12a:Tnos (GB2479)
Plasmid#160629PurposeTU for the expression of LbCas12aDepositorInsertAtUBQ10-:LbCpf1(Cas12a):Tnos
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterUBQ10Available sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
Flag-neo-PP1-Alpha-H248K
Plasmid#171268PurposeMammalian expression of 3x-Flag-tagged human PP1 alpha catalytic subunit H248K mutant that loses enzymatic activityDepositorInsert3x-Flag-tagged human PP1 alpha catalytic subunit H248K mutant (PPP1CA Human)
UseTagsFlagExpressionMammalianMutationPPP1CA-His248Lys (H248K)PromoterCMVAvailable sinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-pre-Ost1-alphaf(I)-E2-Crimson
Plasmid#117661PurposeTest intracellular localisation of E2-Crimson and study the secretion efficiencyDepositorInsertpre-Ost1-alphaf(I)-E2-Crimson
UseTagsExpressionYeastMutationThis plasmid encodes the fluorescent protein E2-C…PromoterpAOX1Available sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
HA-HIF1alpha P402A/P564A-pBabe-puro
Plasmid#19005DepositorInsertHypoxia inducible factor 1 alpha (HIF1A Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationcDNA changed amino acids: Proline 402 to Alanin…PromoterAvailable sinceAug. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
Px461-Cas9n-Trp53-sgRNA-alpha
Plasmid#88846PurposeCRISPR KO of Trp53DepositorInsertTrp53 (Trp53 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1-AtUBQ10:HR1b-GFP-T35S
Plasmid#211837PurposePlant transient expression cassette for HR1b-GFP fusion under UBQ10 promoter.DepositorInsertHR1b
UseSynthetic BiologyTagsGFPExpressionPlantMutationPromoterUBIQUITIN10Available sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
PCDNA3 Flag p38 alpha (agf)
Plasmid#20352DepositorInsertpDCNA3 Flag p38 alpha (agf) (Mapk14 Mouse)
UseTagsFlagExpressionMammalianMutationReplace Thr 180 and Tyr 182 with Ala and Phe resp…PromoterAvailable sinceApril 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
Stat1 alpha Flag pRc/CMV
Plasmid#8691DepositorInsertSTAT1 alpha (STAT1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceFeb. 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
HA-HIF2alpha-P405A/P531A-pBabe-puro
Plasmid#19006DepositorInsertHypoxia inducible factor 2 alpha (EPAS1 Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationcDNA changed amino acids: P405A and P531APromoterAvailable sinceAug. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-pre-pro-alphaf-Btl2
Plasmid#117664PurposeStudy secretion efficiency of Btl2DepositorInsertpre-pro-alphaf-Btl2
UseTagsExpressionYeastMutationThis plasmid encodes the lipase Btl2 together wit…PromoterpAOX1Available sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAG426-GAL-Alpha-synuclein-E46K
Plasmid#181723PurposeGalactose inducible expression of Alpha-synuclein-E46KDepositorInsertAlpha-synuclein (SNCA Human)
UseTagsExpressionYeastMutationE46KPromoterAvailable sinceJuly 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Galpha-i3 G183S ONE-GO
Plasmid#204338PurposeGPCR/G protein BRET biosensorDepositorInsertGalpha-i3 G183S ONE-GO
UseLentiviralTagsExpressionMutationG183S mutation in Galpha-i3 (renders it RGS insen…PromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-mouse PKA-RIIalpha-mEGFP
Plasmid#45527DepositorInsertPKA regulatory subunit RII alpha tagged by mEGFP
UseTagsHisExpressionMammalianMutationPromoterAvailable sinceJune 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-mouse PKA-RIalpha-mEGFP
Plasmid#45525DepositorInsertPKA regulatory subunit RI alpha tagged by mEGFP
UseTagsHisExpressionMammalianMutationPromoterAvailable sinceJune 12, 2013AvailabilityAcademic Institutions and Nonprofits only