Addgene

We narrowed to 9,176 results for: Coli

Showing: 2151 - 2200 of 9176 results

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pZE27GFP3D

Plasmid

#75462

Purpose

Plasmid for constitutive expression of gfp-pdt3D

Depositor

James Collins

Article

Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

Insert

gfp-pdt#3D

Use

Tags

Expression

Bacterial

Mutation

Promoter

placIq promoter

Available since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pZE27GFP0

Plasmid

#75453

Purpose

Plasmid for constitutive expression of gfp-pdt

Depositor

James Collins

Article

Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

Insert

gfp-pdt

Use

Tags

Expression

Bacterial

Mutation

Promoter

placIq promoter

Available since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pZE27GFP2

Plasmid

#75455

Purpose

Plasmid for constitutive expression of gfp-pdt2

Depositor

James Collins

Article

Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

Insert

gfp-pdt#2

Use

Tags

Expression

Bacterial

Mutation

Promoter

placIq promoter

Available since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pZE27GFP4

Plasmid

#75457

Purpose

Plasmid for constitutive expression of gfp-pdt4

Depositor

James Collins

Article

Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

Insert

gfp-pdt#4

Use

Tags

Expression

Bacterial

Mutation

Promoter

placIq promoter

Available since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pCVD315

Plasmid

#63737

Purpose

Derivative of pKO-1 carrying the multiple cloning region from M13mp19RF

Depositor

James Kaper

Article

Vimr et al J Bacteriol. 1988 Apr;170(4):1495-504.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 6, 2015

Availability

Academic Institutions and Nonprofits only
pTGV-Express SMR5448

Plasmid

#28031

Depositor

Susan Rosenberg

Article

Gumbiner-Russo et al Gene. 2001 Jul 25. 273(1):97-104.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 18, 2011

Availability

Academic Institutions and Nonprofits only
pTGV-Light SMR5452

Plasmid

#28032

Depositor

Susan Rosenberg

Article

Gumbiner-Russo et al Gene. 2001 Jul 25. 273(1):97-104.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 20, 2011

Availability

Academic Institutions and Nonprofits only

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pTGV-CAT SMR5453

Plasmid

#28033

Depositor

Susan Rosenberg

Article

Gumbiner-Russo et al Gene. 2001 Jul 25. 273(1):97-104.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 20, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pTGV-Express-Tet SMR5918

Plasmid

#28035

Depositor

Susan Rosenberg

Article

Gumbiner-Russo et al Gene. 2001 Jul 25. 273(1):97-104.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 20, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pTGV-ProK Express SMR5454

Plasmid

#28034

Depositor

Susan Rosenberg

Article

Gumbiner-Russo et al Gene. 2001 Jul 25. 273(1):97-104.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 12, 2011

Availability

Academic Institutions and Nonprofits only

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pTGV-Express-Kan SMR5917

Plasmid

#28038

Depositor

Susan Rosenberg

Article

Gumbiner-Russo et al Gene. 2001 Jul 25. 273(1):97-104.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 15, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pKOV

Plasmid

#25769

Depositor

George Church

Article

Link et al J Bacteriol. 1997 Oct . 179(20):6228-37.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 3, 2010

Availability

Academic Institutions and Nonprofits only

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ptac::ccdB

Plasmid

#15922

Depositor

Nina Papavasiliou

Article

Besmer et al Mol Cell Biol. 2006 Jun . 26(11):4378-85

Insert

ccdB (ccdB E. coli)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 26, 2007

Availability

Academic Institutions and Nonprofits only

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ClpP-his

Plasmid

#22144

Depositor

Robert Sauer

Article

Martin et al Nature. 2005 Oct 20. 437(7062):1115-20.

Insert

ClpP-His6

Use

Tags

his

Expression

Bacterial

Mutation

C-terminal His tag

Promoter

Available since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21a-BirA

Plasmid

#20857

Depositor

Alice Ting

Article

Howarth et al Proc Natl Acad Sci U S A. 2005 May 24. 1

Insert

BirA

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

April 21, 2009

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-p15

Plasmid

#62656

Purpose

Arabinose inducible lambda red and anhydrotetracycline inducible sgRNA expression that targets pCas9 plasmid

Depositor

Kristala Prather

Article

Reisch et al Sci Rep. 2015 Oct 14;5:15096. doi: 10.10

Insert

exo, beta, gam, sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pUltra-chPheRS

Plasmid

#182257

Purpose

Genetic Incorporation of DOPA

Depositor

Han Xiao

Article

Chen et al J Mol Biol. 2021 Dec 20:167412. doi: 10.

Inserts

chPheRS2 for DOPA
chPheT for DOPA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-tTA

Plasmid

#104102

Purpose

Expresses tTA2 under the CAG promoter in mammalian cells.

Depositor

Takeshi Imai

Article

Sakaguchi et al eLife. 2018 Nov 20;7. pii: 40350. doi: 1

Insert

tTA2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pKD4

Plasmid

#45605

Depositor

Barry L. Wanner

Article

Datsenko et al Proc Natl Acad Sci U S A. 2000 Jun 6;97(

Insert

FRT-kan-FRT

Use

Tags

Expression

Bacterial

Mutation

Promoter

R6K

Available since

June 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pJMK001

Plasmid

#33780

Depositor

Adam Cohen

Article

Kralj et al Science. 2011 Jul 15;333(6040):345-8.

Insert

Proteorhodopsin Optical Proton Sensor

Use

Tags

Expression

Bacterial

Mutation

D97N

Promoter

AraBAD

Available since

Dec. 14, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pKD3

Plasmid

#45604

Depositor

Barry L. Wanner

Article

Datsenko et al Proc Natl Acad Sci U S A. 2000 Jun 6;97(

Insert

FRT-cat-FRT

Use

Tags

Expression

Bacterial

Mutation

Promoter

R6K

Available since

June 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pTR47m4-GFP

Plasmid

#102436

Purpose

Formaldehyde biosensor plasmid with sGFP reporter and modified frm promoter

Depositor

Gregory Stephanopoulos

Article

Woolston et al Biotechnol Bioeng. 2017 Sep 16. doi: 10.

Inserts

Formaldehyde repressor protein (frmR)
Superfolder GFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCas9-CR4

Plasmid

#62655

Purpose

Cas9 nuclease under control of pTet promoter with ssrA tag and constitutive tetR

Depositor

Kristala Prather

Article

Reisch et al Sci Rep. 2015 Oct 14;5:15096. doi: 10.10

Insert

Cas9 nuclease

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pBSJL1

Plasmid

#71261

Purpose

E. coli-Veillonella shuttle plasmid

Depositor

Felicia Qi

Article

Liu et al Appl Environ Microbiol. 2012 May;78(9):3

Type

Empty backbone

Use

E. coli-veillonella shuttle plasmid

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only
pET28a-6xHis-MBP-TEV site-TadA8r

Plasmid

#208318

Purpose

Overxpression MBP-TadA8r

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA8r

Use

Tags

Expression

Bacterial

Mutation

mutations in TadA are described in the paper

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pFD152

Plasmid

#125546

Purpose

derivative of pFD116 with an optimized RBS for E. coli

Depositor

David Bikard

Article

Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)

Insert

optimized RBS

Use

CRISPR

Tags

Expression

Mutation

Promoter

anhydrotetracycline Ptet promoter

Available since

Aug. 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET11_Ec_ORF209

Plasmid

#69029

Purpose

Expresses E.coli ORF209/ADPRase in bacterial cells

Depositor

Sandra Gabelli, Maurice Bessman

Article

Gabelli et al Nat Struct Biol. 2001 May;8(5):467-72.

Insert

ORF209/ADPRase

Use

Tags

-

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 2, 2015

Availability

Academic Institutions and Nonprofits only
pBbA5k-acrAB-sfGFP

Plasmid

#195516

Purpose

Inducible expression vector for transcriptionally fused acrAB and sfGFP.

Depositor

Mary Dunlop

Article

Wen et al Sci Rep. 2018 Oct 11;8(1):15120. doi: 10

Insert

acrAB

Use

Synthetic Biology

Tags

sfgfp

Expression

Bacterial

Mutation

Promoter

lacUV5

Available since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-6xHis-MBP-TEV site-TadA8e

Plasmid

#208319

Purpose

Overxpression MBP-TadA8e

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA8e

Use

Tags

Expression

Bacterial

Mutation

mutations in TadA are described in the paper

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCVD364

Plasmid

#63739

Purpose

V cholerae 395 nanH gene cloned into the BamHI site of pCVD315

Depositor

James Kaper

Article

Vimr et al J Bacteriol. 1988 Apr;170(4):1495-504.

Insert

nanH

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 6, 2015

Availability

Academic Institutions and Nonprofits only
pMo168

Plasmid

#27389

Depositor

Martin Voskuil

Article

Hamad et al Gene. 2009 Feb 1. 430(1-2):123-31.

Insert

pMo168

Use

Tags

Expression

Bacterial

Mutation

A replicative vector for gene expression in Burkh…

Promoter

Available since

March 7, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-Syn1-tTA

Plasmid

#104109

Purpose

AAV-mediated expression of tTA under the Syn1 promoter.

Depositor

Takeshi Imai

Article

Sakaguchi et al eLife. 2018 Nov 20;7. pii: 40350. doi: 1

Insert

tTA2

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn1

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pdCas9-sgRNA-RFP

Plasmid

#166005

Purpose

Bacterial expression of dCas9 (aTc control) and sgRNA (arabinose control)

Depositor

Martin Buck

Article

Bradley et al Biotechnol Rep (Amst). 2021 Oct 9;32:e00

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 27, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDW363

Plasmid

#8842

Depositor

David Waugh

Article

Tsao et al Gene. 1996 Feb 22. 169(1):59-64.

Type

Empty backbone

Use

Tags

MBP and biotin acceptor peptide

Expression

Bacterial

Mutation

Promoter

Available since

April 19, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSETa mEos2

Plasmid

#20341

Purpose

bacterial expression of an EosFP variant, mEos2 (a photoconvertible fluorescent protein)

Depositor

Loren Looger

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

mEos-2

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pPR219.05

Plasmid

#78554

Purpose

Plasmid expressing UirR, response regulator and PcsiR1-regulated GFP output

Depositor

Jeffrey Tabor

Article

Ramakrishnan et al ACS Synth Biol. 2016 Apr 27.

Insert

uirR

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

J23115

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pKB224

Plasmid

#170014

Purpose

Encodes LacI-controlled expression of surface-displayed NC and PvirK-mNeonGreen reporter

Depositor

Jeffrey Tabor

Article

Brink et al bioRxiv

Inserts

mNeonGreen
NC

Use

Synthetic Biology

Tags

Lpp, OmpA, and Tether

Expression

Bacterial

Mutation

Promoter

Ptac and PvirK

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPkan-S2

Plasmid

#61601

Purpose

PCR template for en passant (two-step Red-mediated) mutagenesis

Depositor

Nikolaus Osterrieder

Article

Tischer et al Biotechniques. 2006 Feb;40(2):191-7.

Insert

Kana_I-SceI

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD33.lon

Plasmid

#22145

Depositor

Robert Sauer

Article

Gur et al Genes Dev. 2008 Aug 15. 22(16):2267-77.

Insert

Lon (lon E. coli K12)

Use

Tags

Expression

Bacterial

Mutation

None, WT gene

Promoter

Available since

Sept. 28, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
PRExpress

Plasmid

#122486

Purpose

A non-leaky heat-inducible gene expression system for Drosophila. (GenBank: MF058598.1)

Depositor

Renato Paro

Article

Akmammedov et al Sci Rep. 2017 Jul 31;7(1):6899. doi: 10.

Inserts

LacZ
poly-PRE

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

May 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-mNaChBacMut-T2A-tdTomato

Plasmid

#60657

Purpose

Expresses mNaChBacMut-T2A-tdTomato

Depositor

Massimo Scanziani

Article

Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

Insert

mNaChBacMut-T2A-tdTomato

Use

Tags

Expression

Mammalian

Mutation

E191K

Promoter

Available since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPkan-S

Plasmid

#41017

Purpose

PCR template for en passant (two-step Red-mediated) mutagenesis

Depositor

Nikolaus Osterrieder

Article

Tischer et al Biotechniques. 2006 Feb;40(2):191-7.

Insert

Kana_I-SceI

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pBSM

Plasmid

#67505

Purpose

Express the tRNAMet scaffold

Depositor

Luc Ponchon

Article

Ponchon et al Nat Protoc. 2009;4(6):947-59. doi: 10.10

Insert

modified tRNAMet

Use

Tags

Expression

Bacterial

Mutation

Promoter

lpp

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
FLIPrbs

Plasmid

#17860

Depositor

Wolf Frommer

Article

Lager et al FEBS Lett. 2003 Oct 9. 553(1-2):85-9.

Insert

FLIPrbs

Use

Tags

ECFP and EYFP

Expression

Bacterial

Mutation

D208G

Promoter

Available since

April 29, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pNK141g2

Plasmid

#156380

Purpose

Expresses orthogonal RBS mPapaya reporter

Depositor

Ahmed Badran

Article

Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

Insert

mPapaya

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pFF753

Plasmid

#61453

Purpose

IPTG-inducible msd(wt)

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

msd(wt)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

pLacO

Available since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pTK204

Plasmid

#13534

Depositor

Wolf Frommer

Article

Kaper et al PLoS Biol. 2007 Oct;5(10):e257.

Insert

FLIPW-CTYT

Use

Tags

See map

Expression

Bacterial

Mutation

Promoter

Available since

July 27, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pYHY1

Plasmid

#215926

Purpose

E. coli-Bacteroides shuttle vector.

Depositor

Shannon Sirk

Article

Yeh et al Nat Commun. 2024 Nov 11;15(1):9741. doi:

Type

Empty backbone

Use

Synthetic Biology; E. coli-bacteroides shuttle ve…

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pFF530

Plasmid

#61447

Purpose

IPTG-inducible msd_kanR(ON)

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

msd_kanR(ON)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

pLacO

Available since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
DVC_p15A_FG

Plasmid

#217590

Purpose

Destination vector with chloramphenicol resistance and p15A origin of replication. Carries F-G CIDAR MoClo overhangs for level 1 construct cloning.

Depositor

Wei Huang

Article

Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

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