We narrowed to 4,233 results for: gca

1. pLCHKOv3-mClover-intergenic-As

   Plasmid

   #209036

   Purpose

   Lentiviral vector expressing mClover3 along with U6 driven hybrid guide (hgRNA) targeting two intergenic sites in the human genome using SpCas9 and AsCas12a nucleases (CHyMErA system), respectively

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   intergenic hgRNA with SpCas9 tracrRNAv1 & AsCas12a DR
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLCHKOv3-mClover-intergenic_2-As

   Plasmid

   #218814

   Purpose

   Lentiviral vector expressing mClover3 along with U6 driven hybrid guide (hgRNA) targeting two intergenic sites in the human genome using SpCas9 and AsCas12a nucleases (CHyMErA system), respectively

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   intergenic hgRNA with SpCas9 tracrRNAv1 & AsCas12a DR
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLCHKOv3-mClover-intergenic_3-As

   Plasmid

   #218815

   Purpose

   Lentiviral vector expressing mClover3 along with U6 driven hybrid guide (hgRNA) targeting two intergenic sites in the human genome using SpCas9 and AsCas12a nucleases (CHyMErA system), respectively

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   intergenic hgRNA with SpCas9 tracrRNAv1 & AsCas12a DR
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLCHKOv3-mClover-intergenic_4-As

   Plasmid

   #218816

   Purpose

   Lentiviral vector expressing mClover3 along with U6 driven hybrid guide (hgRNA) targeting two intergenic sites in the human genome using SpCas9 and AsCas12a nucleases (CHyMErA system), respectively

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   intergenic hgRNA with SpCas9 tracrRNAv1 & AsCas12a DR
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. DGKE gRNA (BRDN0001147022)

   Plasmid

   #76609

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human DGKE

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   DGKE (DGKE Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pU6-pegRNA-HEK3-CTTins-px330-scaffold

   Plasmid

   #180017

   Purpose

   Transiently expressing a pegRNA to introduce HEK3 CTT insertion in human cells. It has a sgRNA scaffold from px330.

   Depositor

   Dirk Hockemeyer

   Article

   Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

   Insert

   Prime editing pegRNA for HEK3-CTTins, with px330 scaffold (EPHA8 Synthetic)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6

   Available since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMXS-IRES-BLAST flag-CAD hCPS1 AD

   Plasmid

   #188130

   Purpose

   Expresses C-terminal flag-tagged human CAD hCPS1 AD in mammalian cells

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   TCC -> AGT silent mutations at nt439-441 elimi…

   Promoter

   Available since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSLED.CaRPv1

   Plasmid

   #193018

   Purpose

   Excitatory/Inhibitory neuron calcium indicator (hSyn promoter, Synrg exon, jRGECO1a/jGCaMP7b)

   Depositor

   Jonathan Ling

   Article

   Ling et al Nat Commun. 2022 Oct 1;13(1):5773. doi:

   Insert

   Bichromatic calcium indicator (jGCaMP7b and jRGECO1a)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Ai148 (TIT2L-GC6f-ICL-tTA2) targeting vector

   Plasmid

   #114428

   Purpose

   Target a Cre-dependent GCaMP6f expression and a tTA2 cassette into the mouse TIGRE locus

   Depositor

   Hongkui Zeng

   Article

   Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

   Insert

   GCaMP6f
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   TRE2, CAG

   Available since

   Jan. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Ai162 (TIT2L-GC6s-ICL-tTA2) targeting vector

    Plasmid

    #114433

    Purpose

    Target a Cre-dependent GCaMP6s cassette and a tTA2 cassette into the mouse TIGRE locus

    Depositor

    Hongkui Zeng

    Article

    Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

    Insert

    GCaMP6s, tTA2
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    TRE2, CAG

    Available since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. AAV-KrasWT/sgKras/Cre

    Plasmid

    #99848

    Purpose

    Expresses Cre-recombinase, a barcoded HDR template that serves as a control for HDR into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.

    Depositor

    Monte Winslow

    Article

    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:

    Insert

    Kras (Kras Mouse)
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…

    Promoter

    Available since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLV-RnSyt1-sgRNA3-dCas9-KRAB-TagBFP2 (Identifier AAAA-0247)

    Plasmid

    #202556

    Purpose

    Synaptotagmin-1 sgRNA3 with TagBFP2

    Depositor

    Frederic Meunier

    Article

    Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

    Insert

    CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ - CACCTCCTCCTGCAGCGGCAGCATCGG) (Syt1 Rat)
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

    Expression

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Ai163 (TIT2L-GC6s-ICL-TPT) targeting vector

    Plasmid

    #114430

    Purpose

    Target a Cre-dependent GCaMP6s cassette and a tdTomato-P2A-tTA2 cassette to the mouse TIGRE locus

    Depositor

    Hongkui Zeng

    Article

    Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

    Insert

    GCaMP6s, tdTomato, tTA2
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    TRE2, CAG

    Available since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. lenti-hygro-sgDDX6-2

    Plasmid

    #240005

    Purpose

    Expresses human sgDDX6 in mammalian cells

    Depositor

    Rui Su

    Insert

    sgDDX6
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. lenti-hygro-sgEIF4E-1

    Plasmid

    #240006

    Purpose

    Expresses human sgEIF4E in mammalian cells

    Depositor

    Rui Su

    Insert

    sgEIF4E
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. SGEP-shRLuc-802

    Plasmid

    #188668

    Purpose

    control shRNA

    Depositor

    Lewis Cantley

    Insert

    shRLuc
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHJL4

    Plasmid

    #199379

    Purpose

    Encodes dual cistronic cytosolic jGCaMP7f and mScarlet-I optimized for C. elegans expression

    Depositor

    Hang Lu

    Insert

    jGCaMP7f::SL2::mScarlet-I
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    no mutations

    Promoter

    Available since

    April 24, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pHJL1

    Plasmid

    #199376

    Purpose

    Encodes dual cistronic cytosolic GCaMP6s and mScarlet-I optimized for C. elegans expression

    Depositor

    Hang Lu

    Insert

    GCaMP6s::SL2::mScarlet-I
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    no mutations

    Promoter

    Available since

    April 24, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pHJL3

    Plasmid

    #199378

    Purpose

    Encodes dual cistronic cytosolic jGCaMP7s and mScarlet-I optimized for C. elegans expression

    Depositor

    Hang Lu

    Insert

    jGCaMP7s::SL2::mScarlet-I
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    no mutations

    Promoter

    Available since

    April 24, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pHJL2

    Plasmid

    #199377

    Purpose

    Encodes dual cistronic cytosolic GCaMP6f and mScarlet-I optimized for C. elegans expression

    Depositor

    Hang Lu

    Insert

    GCaMP6f::SL2::mScarlet-I
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    no mutations

    Promoter

    Available since

    April 24, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge