We narrowed to 7,940 results for: mme

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pCas3-CDA1

Plasmid

#229534

Purpose

pMV_hyg encoding Cas3(wt)-CDA1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning

Depositor

Kevin Verstrepen

Article

Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

Inserts

Cas3
Cytidine deaminase
Uracil glycosylase inhibitor

Tags

XTEN

Expression

Bacterial and Yeast

Mutation

codon optimized for S.cerevisiae and codon optimi…

Available Since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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CROP-seq-copGFP-v2-no-scaffold

Plasmid

#228520

Purpose

CROP-seq-puro-v2 with scaffold sequence removed for the insertion of CRISPRmap Opool with guide, scaffold and barcode sequences, using copGFP as selection marker

Depositor

Jellert Gaublomme

Article

Gu et al Nat Biotechnol. 2024 Oct 7. doi: 10.1038

Type

Empty backbone

Use

Lentiviral

Available Since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pGL4.15_pT-REX17

Plasmid

#195497

Purpose

Firefly luciferase promoter expression vector driven only by the promoter element of human lncRNA T-REX17

Depositor

Alexander Meissner

Article

Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

Insert

Promoter region of lncRNA T-REX17

Tags

luc2-hPEST

Expression

Mammalian

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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LR (hsp70I: cPLA2-mK2)

Plasmid

#162564

Purpose

Measuring cPLA2 activation in live cells after heat shock.

Depositor

Philipp Niethammer

Article

Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

Inserts

Heat shock protein 70 (HSPA1A Human)
cytosolic phospholipase a2 (pla2g4aa Zebrafish)

Use

Gateway Cloning

Tags

mK2

Promoter

cmlc2

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pBiex PKC - Peptide 14 mCit mCer

Plasmid

#83565

Purpose

Expresses a mCitrine-mCerulean FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the pseudosubstrate of PKCalpha.

Depositor

Sivaraj Sivaramakrishnan

Article

Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.

Insert

PKC alpha (PRKCA Human)

Tags

10 nm ER/K Helix, FLAG Purificaiton Tag, Tev Prot…

Expression

Bacterial and Insect

Mutation

Only catalytic domain of PKC (aa 335-672)

Promoter

T7 Promoter

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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Anti-GFP [N86/38.1R]

Plasmid

#114492

Purpose

Mammalian Expression Plasmid of anti-GFP. Derived from hybridoma N86/38.1.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-GFP (Aequorea victoria) recombinant mouse monoclonal antibody

Expression

Mammalian

Promoter

dual CMV

Available Since

May 28, 2019

Availability

Academic Institutions and Nonprofits only

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GFP scFv [N86/38]

Plasmid

#182095

Purpose

Mammalian Expression of GFP scFv. Derived from hybridoma N86/38.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting GFP

Tags

HA, His

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pMA7-SacB

Plasmid

#79968

Purpose

TM-MAGE strain

Depositor

Morten Sommer

Article

Lennen et al Nucleic Acids Res. 2016 Feb 29;44(4):e36

Inserts

Lambda Red recombinase beta subunit
DNA adenine methylase
Levansucrase

Use

Synthetic Biology

Expression

Bacterial

Promoter

pBAD

Available Since

Sept. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pX459_V2.0_pSpCas9(BB)-2A-Puro_T-REX17_Ex1_KI

Plasmid

#195504

Purpose

Cas9-2A-Puro expression vector bearing a sgRNA targeting Exon 1 of human lncRNA T-REX17

Depositor

Alexander Meissner

Article

Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

Insert

sgRNA

Use

CRISPR

Tags

3XFLAG, Puro, and T2A

Expression

Mammalian

Promoter

U6

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH-cGAS-E225A D227A-mCherry2-EF1-Puro

Plasmid

#132771

Purpose

Interacts with cytoplasmic DNA. Useful for identifying sites of nuclear envelope rupture.

Depositor

Jan Lammerding

Article

Denais et al Science. 2016 Apr 15;352(6283):353-8. do

Insert

Cyclic GMP-AMP synthase (CGAS Human)

Use

Lentiviral

Tags

mCherry2

Expression

Mammalian

Mutation

E225A D227A

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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GST scFv [N100/13]

Plasmid

#190521

Purpose

Mammalian Expression of GST scFV. Derived from hybridoma N100/13.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

GST (Schistosoma japonicum) recombinant scFV

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-Pannexin-2 [N121A/31R]

Plasmid

#140073

Purpose

Mammalian Expression Plasmid of anti-Pannexin-2 (Rat). Derived from hybridoma N121A/31.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Pannexin-2 (Rat) recombinant mouse monoclonal antibody (Panx2 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

June 24, 2020

Availability

Academic Institutions and Nonprofits only

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GFP scFv [N86/20]

Plasmid

#206744

Purpose

Mammalian Expression of GFP scFV. Derived from hybridoma N86/20 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

GFP (Aequorea victoria) recombinant scFV

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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GFP scFv [N86/44]

Plasmid

#206743

Purpose

Mammalian Expression of GFP scFV. Derived from hybridoma N86/44 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

GFP (Aequorea victoria) recombinant scFV

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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GFP scFv [N86/8]

Plasmid

#199429

Purpose

Mammalian expression of GFP scFv. Derived from hybridoma N86/8.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targetingGFP (Aequorea victoria)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pYFP-ErbB2

Plasmid

#66948

Purpose

Mammalian expression of rat ErbB2 tagged with YFP

Depositor

Mads Lerdrup, Bo van_Deurs

Article

Lerdrup et al Mol Biol Cell. 2007 Sep;18(9):3656-66. E

Insert

ErbB2 (Erbb2 Rat)

Tags

6xHis, V5 Tag, and YFP

Expression

Mammalian

Mutation

(See depositor comment below on mutations A24T an…

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
pFRLuc-CoV2-FLucStop

Plasmid

#177617

Purpose

Dual luciferase reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. In-frame stop codon inserted after FLuc (negative control).

Depositor

Junjie Guo

Article

Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11

Insert

SARS-CoV-2 FSE

Use

Luciferase

Expression

Mammalian

Mutation

In-frame stop codon added between FLuc and FSE

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pMX_mTAF3

Plasmid

#44334

Depositor

Marc Timmers

Article

Pijnappel et al Nature. 2013 Mar 28;495(7442):516-9. doi

Insert

TAF3 (Taf3 Mouse)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Promoter

LTR

Available Since

May 14, 2013

Availability

Academic Institutions and Nonprofits only

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pETDuet-1 Flag-LplA

Plasmid

#232118

Purpose

For bacterial expression of Flag-tagged E. coli LplA.

Depositor

Ruth Sommese

Article

Liu et al J Biol Chem. 2023 Mar;299(3):102959. doi

Insert

lplA (lplA Escherichia coli (strain K12))

Tags

Flag

Expression

Bacterial

Promoter

T7 Promoter

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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Anti-PICK1 [L20/8R]

Plasmid

#128630

Purpose

Mammalian Expression Plasmid of anti-PICK1 (Human). Derived from hybridoma L20/8.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-PICK1 (Human) recombinant mouse monoclonal antibody (PICK1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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