We narrowed to 8,679 results for: sgRNA

1. pVC-Ds-DrU6a:scrambled3-sgRNA-Ds

   Plasmid

   #119072

   Purpose

   Construct with scrambled-3 sgRNA sequence for microinjections in zebrafish

   Depositor

   Tatjana Sauka-Spengler

   Article

   Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

   Insert

   U6a:scrambled3-sgRNA
   

   Expression

   Bacterial

   Promoter

   Zebrafish U6a promoter

   Available Since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pVC-Ds-DrU6a:scrambled4-sgRNA-Ds

   Plasmid

   #119073

   Purpose

   Construct with scrambled-4 sgRNA sequence for microinjections in zebrafish

   Depositor

   Tatjana Sauka-Spengler

   Article

   Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

   Insert

   U6a:scrambled4-sgRNA
   

   Expression

   Bacterial

   Promoter

   Zebrafish U6a promoter

   Available Since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA-H1-sgRNA-mTZAP

   Plasmid

   #87187

   Purpose

   guideRNA targeting exon2 of mouse TZAP

   Depositor

   Eros Lazzerini Denchi

   Article

   Li et al Science. 2017 Jan 12. pii: aah6752. doi:

   Insert

   TZAP (Zbtb48 Mouse)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   H1

   Available Since

   March 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. sgRNA(MS2)_Prnp_SAM2

   Plasmid

   #78625

   Purpose

   Encodes sgRNA2.0 targeting 138nt upstream of TSS

   Depositor

   Walker Jackson

   Article

   Kaczmarczyk et al PLoS One. 2016 Apr 29;11(4):e0154604. do

   Insert

   sgRNA(MS2)_Prnp_SAM2 (Prnp Mouse)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   July 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. sgRNA(MS2)_Prnp_SAM1

   Plasmid

   #78624

   Purpose

   Encodes sgRNA2.0 targeting 158nt upstream of TSS

   Depositor

   Walker Jackson

   Article

   Kaczmarczyk et al PLoS One. 2016 Apr 29;11(4):e0154604. do

   Insert

   sgRNA(MS2)_Prnp_SAM2 (Prnp Mouse)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   July 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. lentiCRISPR - C16orf80 sgRNA 1

   Plasmid

   #70651

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C16orf80 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

   Depositor

   David Sabatini

   Article

   Wang et al Science. 2015 Oct 15. pii: aac7041.

   Insert

   sgRNA against C16orf80
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Jan. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

7. lentiCRISPR - C16orf80 sgRNA 2

   Plasmid

   #70650

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C16orf80 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

   Depositor

   David Sabatini

   Article

   Wang et al Science. 2015 Oct 15. pii: aac7041.

   Insert

   sgRNA against C16orf80
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Jan. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

8. lentiCRISPR - C3orf17 sgRNA 2

   Plasmid

   #70653

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C3orf17 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

   Depositor

   David Sabatini

   Article

   Wang et al Science. 2015 Oct 15. pii: aac7041.

   Insert

   sgRNA against C3orf17
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

9. sgRNA1_pNeurog2-Luciferase reporter

   Plasmid

   #64160

   Purpose

   Photoactivatable transcription system. Mammalian expression of sgRNA1 to target pNeurog2-Luciferase reporter.

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   sgRNA1 for pNeurog2-Luciferase reporter
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   human U6

   Available Since

   June 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAN-PBAD-sgRNA-VR

    Plasmid

    #62286

    Purpose

    expression of VR sgRNA from the arabinose-inducible promoter

    Depositor

    Christopher Voigt

    Article

    Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

    Insert

    VR sgRNA
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-FLEX-SaCas9-U6-sgRNA

    Plasmid

    #124844

    Purpose

    Vector for Cre-dependent expression of SaCas9

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    SaCas9, gRNA scaffold
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    NLS and NLS-3xHA

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. tet-pLKO-sgRNA-puro

    Plasmid

    #104321

    Purpose

    3rd generation lentiviral plasmid for inducible expression of sgRNA; derived from tet-pLKO-puro; puromycin selection. See manual for detailed protocols.

    Depositor

    Nathanael Gray

    Article

    Huang et al eLife. 2017 Sep 19;6. pii: e26693. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    H1/TO

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV sgRNA-Notch1 #1 GFP

    Plasmid

    #106950

    Purpose

    Lentivirus encoding sgRNA targeting murine Notch1. Includes GFP marker.

    Depositor

    Albert Edge

    Article

    Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

    Insert

    gRNA targeting Notch1 (Notch1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV sgRNA-Hic1 GFP

    Plasmid

    #106953

    Purpose

    Lentivirus encoding sgRNA targeting murine Hic1. Includes GFP marker.

    Depositor

    Albert Edge

    Article

    Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

    Insert

    anti-Hic1 sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUF-Cas9-pre-sgRNA

    Plasmid

    #137845

    Purpose

    Cas9 and gRNA expression plasmid for P. falciparum with yDHODH selectable marker.

    Depositor

    Josh Beck

    Article

    Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    May 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. sgRNA(MS2) cloning backbone

    Plasmid

    #61424

    Purpose

    sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-U6-sgRNA-CMV-GFP

    Plasmid

    #85451

    Purpose

    Express sgRNA in mammalian cells

    Depositor

    Hetian Lei

    Article

    Duan et al J Biol Chem. 2016 May 31. pii: jbc.M116.

    Insert

    pCMV-EGFP
    

    Use

    AAV

    Promoter

    pCMV-EGFP

    Available Since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMS79 (eft-3p::Cas9 + sgRNA)

    Plasmid

    #154839

    Purpose

    Cas9 + sgRNA plasmid that is targeted to the synthetic guide sequence GGACAGTCCTGCCGAGGTGG

    Depositor

    Patrick Phillips

    Article

    Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

    Insert

    sgRNA
    

    Use

    CRISPR

    Expression

    Worm

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMSCV-U6sgRNA(BbsI)-PGKpuro2ABFP

    Plasmid

    #102796

    Purpose

    Retrovirus for delivery of one sgRNA (empty, bbsI)

    Depositor

    Sarah Teichmann

    Article

    Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

    Insert

    U6 sgRNA
    

    Use

    CRISPR and Retroviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHR-U6-sgRNA-CMV-puro

    Plasmid

    #169450

    Purpose

    backbone for sgRNA expression

    Depositor

    Stanley Qi

    Article

    Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

    Insert

    sgRNA
    

    Use

    Lentiviral

    Available Since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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