Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 1,075 results for: sri

Showing: 861 - 880 of 1075 results

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pET28a(+)-NEDD4L_W3-W4-HECT_C874only

Plasmid

#187711

Purpose

Express his-tagged NEDD4L W3-W4-HECT_C874only in bacterial cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 492-975 with mutations (NEDD4L Human)

Tags

His-HRV 3C site

Expression

Bacterial

Mutation

deletion of residues 1-491; mutations C702A, C776…

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-NEDD4L_Δ1,2-linker

Plasmid

#187714

Purpose

Express GST-tagged NEDD4L FL with removal of the linker between the WW1 and WW2 domains in bacterial cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L with residues 226-382 removed (NEDD4L Human)

Tags

GST-HRV 3C site

Expression

Bacterial

Mutation

deletion of residues 226-382

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-NEDD4L_Δ2,3-linker

Plasmid

#187715

Purpose

Express GST-tagged NEDD4L FL with removal of the linker between the WW2 and WW3 domains in bacterial cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L with residues 418-495 removed (NEDD4L Human)

Tags

GST-HRV 3C site

Expression

Bacterial

Mutation

deletion of residues 418-495

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-HHEX

Plasmid

#222928

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of HHEX

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

HHEX (HHEX Human)

Expression

Mammalian

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pET28 mOct4-POU

Plasmid

#206393

Purpose

Expresses the DNA binding domain of the Oct4 with a 6xHis tag for protein purification

Depositor

Ralf Jauch

Article

Tan et al Mol Biol Evol. 2021 Jun 25;38(7):2854-28

Insert

Oct4 (Pou5f1 Mouse)

Tags

6xHis

Expression

Bacterial

Mutation

POU DNA binding domain only

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR201-Eps8L2_494-546

Plasmid

#196531

Purpose

Entry vector for Gateway with Eps8L2_494-546

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_494-546 (EPS8L2 Human)

Expression

Bacterial

Promoter

CMV promoter; T7 promoter

Available Since

July 21, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR201-Eps8L2_367-496

Plasmid

#196530

Purpose

Entry vector for Gateway with Eps8L2_367-496

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_367-496 (EPS8L2 Human)

Expression

Bacterial

Promoter

CMV promoter; T7 promoter

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR201-Eps8L2_1-183

Plasmid

#196527

Purpose

Entry vector for Gateway with Eps8L2_1-183

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_1-183 (EPS8L2 Human)

Expression

Bacterial

Promoter

CMV promoter; T7 promoter

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR201-Eps8L2_544-715

Plasmid

#196532

Purpose

Entry vector for Gateway with Eps8L2_544-715

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_544-715 (EPS8L2 Human)

Expression

Bacterial

Promoter

CMV promoter; T7 promoter

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR201-Eps8L2_181-299

Plasmid

#196528

Purpose

Entry vector for Gateway with Eps8L2_181-299

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_181-299 (EPS8L2 Human)

Expression

Bacterial

Promoter

CMV promoter; T7 promoter

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-Eps8L2_1-496

Plasmid

#196516

Purpose

Expression of GST-Eps8L2_1-496

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_1-496 (EPS8L2 Human)

Tags

GST

Expression

Bacterial

Mutation

Silent mutations for siRNA resistance

Promoter

lac promoter

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST15-Eps8L2_1-183

Plasmid

#196535

Purpose

Expression of GST-Eps8L2_1-183

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_1-183 (EPS8L2 Human)

Tags

GST

Expression

Bacterial

Promoter

T7 promoter; lac UV5 promoter

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST15-Eps8L2_181-299

Plasmid

#196536

Purpose

Expression of GST-Eps8L2_181-299

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_181-299 (EPS8L2 Human)

Tags

GST

Expression

Bacterial

Promoter

T7 promoter; lac UV5 promoter

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST15-Eps8L2_544-715

Plasmid

#196540

Purpose

Expression of GST-Eps8L2_544-715

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_544-715 (EPS8L2 Human)

Tags

GST

Expression

Bacterial

Promoter

T7 promoter; lac UV5 promoter

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Nmyc-Eps8L2-FL-siR

Plasmid

#196514

Purpose

Expression of Myc-Eps8L2-FL

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2-FL-siR (EPS8L2 Human)

Tags

Myc

Expression

Mammalian

Mutation

Silent mutations for siRNA resistance

Promoter

T7 promoter

Available Since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR201-Eps8L2_529-715

Plasmid

#196533

Purpose

Entry vector for Gateway with Eps8L2_529-715

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_529-715 (EPS8L2 Human)

Expression

Bacterial

Promoter

CMV promoter; T7 promoter

Available Since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST15-Eps8L2_297-370

Plasmid

#196537

Purpose

Expression of GST-Eps8L2_297-370

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_297-370 (EPS8L2 Human)

Tags

GST

Expression

Bacterial

Promoter

T7 promoter; lac UV5 promoter

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST15-Eps8L2_367-496

Plasmid

#196538

Purpose

Expression of GST-Eps8L2_367-496

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_367-496 (EPS8L2 Human)

Tags

GST

Expression

Bacterial

Promoter

T7 promoter; lac UV5 promoter

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST15-Eps8L2_494-546

Plasmid

#196539

Purpose

Expression of GST-Eps8L2_494-546

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_494-546 (EPS8L2 Human)

Tags

GST

Expression

Bacterial

Promoter

T7 promoter; lac UV5 promoter

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pS97splitRBP_S1dC2

Plasmid

#183769

Purpose

Expresses SHIP1-delta-C2-Enzyme domain flanked by RBP

Depositor

William Kerr

Article

Sudan et al Front Immunol. 2022 May 4;13:830961. doi

Insert

SHIP1deltaC2 (INPP5D Human)

Tags

6HIS, RBP (C-terminal) w/HRV3C cleavage site, and…

Expression

Bacterial

Mutation

SHIP1deltaC2 includes aa N393 to aa Q730 only

Promoter

T7lac

Available Since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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