We narrowed to 12,197 results for: 112

1. pGEX-4T-1 3xFlag-p38alpha

   Plasmid

   #47575

   Purpose

   Bacterial expression plasmid for GST-3xFlag-tagged p38alpha

   Depositor

   Kevin Janes

   Article

   Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

   Insert

   p38alpha (Mapk14 Mouse)
   

   Tags

   3xFlag and GST

   Expression

   Bacterial

   Available Since

   Aug. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

2. NPY1R-DuET

   Plasmid

   #213354

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   NPY1R (NPY1R Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMV-dLight1.2

   Plasmid

   #111054

   Purpose

   Expresses dLight1.2 in mammalian cells

   Depositor

   Lin Tian

   Article

   Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

   Insert

   dLight1.2
   

   Tags

   Flag tag

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Sept. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. mouse CENP-B-EGFP

   Plasmid

   #107264

   Purpose

   mouse CENP-B-EGFP in pIVT vector for in vitro transcription

   Depositor

   Michael Lampson

   Article

   Akera et al Science. 2017 Nov 3;358(6363):668-672. d

   Insert

   CENP-B-EGFP (Cenpb Mouse)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   Sept. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLC-mCherry-MLKL

   Plasmid

   #75167

   Purpose

   LentiCRISPR-mCherry with sgRNA targeting human MLKL

   Depositor

   Beat Bornhauser

   Article

   McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

   Insert

   MLKL sgRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA

   Plasmid

   #50942

   Purpose

   Bicistronic vector expressing mRuby2 and GCaMP6s from a single open reading frame.

   Depositor

   Tobias Rose, Tobias Bonhoeffer, Mark Huebener

   Article

   Rose et al Science. 2016 Jun 10;352(6291):1319-22.

   Has Service

   AAV1

   Insert

   mRuby2-P2A-GCaMP6s
   

   Use

   AAV

   Promoter

   hSyn1

   Available Since

   March 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pQE-meGFP-DLC1

   Plasmid

   #192048

   Purpose

   Plasmid expresses DLC1 binding peptide to Talin R8 domain in chimera with meGFP

   Depositor

   Ionel Popa

   Article

   Dahal et al Sci Adv. 2022 Jul 15;8(28):eabl7719. doi

   Insert

   DLC1 (DLC1 Human)
   

   Expression

   Bacterial

   Available Since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFUW-tetO-Myb

   Plasmid

   #139826

   Purpose

   doxycycline-inducible expression of mouse Myb in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

   Insert

   Myb (Myb Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   P94H and Q619R- Please see depositor comment belo…

   Promoter

   TRE-mCMV

   Available Since

   April 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. MF64_PB-TRE3G-6x42bs-6x(37bs_37bs)-miniCMV-NLS-FKBP12F36V-37ZFR2AR11AR39AR67A-VP16-NLS-DHFR-IRES-mCitrine-PEST-BGHpA

   Plasmid

   #176624

   Purpose

   TF B self-activation cassette in MultiFate-2.1 and MultiFate-2.3

   Depositor

   Michael Elowitz

   Article

   Zhu et al Science. 2022 Jan 21;375(6578):eabg9765.

   Insert

   TRE3G-6x42bs-6x(37bs_37bs)-miniCMV-NLS-FKBP12F36V-37ZFR2AR11AR39AR67A-VP16-NLS-DHFR-IRES-mCitrine-PEST-BGHpA
   

   Use

   Synthetic Biology

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. MF85_PB-14xUAS-12x42bs-10x(ErbB2bs_ErbB2bs)-miniCMV-NLS-FKBP12F36V-ErbB2ZFR2AR39A-VP16-NLS-DHFR-IRES-mTurquoise2-PEST-BGHpA

    Plasmid

    #176629

    Purpose

    TF C self-activation cassette in MultiFate-3

    Depositor

    Michael Elowitz

    Article

    Zhu et al Science. 2022 Jan 21;375(6578):eabg9765.

    Insert

    14xUAS-12x42bs-10x(ErbB2bs_ErbB2bs)-miniCMV-NLS-FKBP12F36V-ErbB2ZFR2AR39A-VP16-NLS-DHFR-IRES-mTurquoise2-PEST-BGHpA
    

    Use

    Synthetic Biology

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCEP4-BG505.SOSIP-8his

    Plasmid

    #111847

    Purpose

    Mammalian expression plasmid for soluble BG505 SOSIP.664

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 Env (BG505 SOSIP.664)
    

    Tags

    8his purification tag and CD5 leader sequence

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene; has SOSIP mutatio…

    Promoter

    CMV

    Available Since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLentiCRISPRv2_mSTING_scrambled_gRNA_2

    Plasmid

    #196628

    Purpose

    Scrambled control 2 for STING knock-out in murine cells.

    Depositor

    Nicolas Manel

    Article

    Jneid et al Sci Immunol. 2023 Jan 13;8(79):eabn6612.

    Insert

    mSTING scrambled gRNA 2
    

    Use

    CRISPR and Lentiviral

    Mutation

    WT

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCAG-MBP-ATG9

    Plasmid

    #188076

    Purpose

    Recombinant overexpression of human ATG9 in mammalian cells

    Depositor

    James Hurley

    Article

    Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

    Insert

    ATG9 (ATG9A Human)
    

    Tags

    MBP tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGEX-4T-1 3xHA-MEK1-DD

    Plasmid

    #47576

    Purpose

    Bacterial expression plasmid for GST-3xHA-tagged constitutively active MEK1

    Depositor

    Kevin Janes

    Article

    Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

    Insert

    MEK1-DD (Map2k1 Mouse)
    

    Tags

    3xHA and GST

    Expression

    Bacterial

    Mutation

    Ser218 to Asp, Ser221 to Asp

    Available Since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pLGB36

    Plasmid

    #135621

    Purpose

    Suicide vector for allelic replacement in Bacteroides species including B. fragilis strain 638R, erythromycin selection and aTC-inducible ss-Bfe3 counterselection

    Depositor

    Laurie Comstock

    Article

    Ito et al mBio. 2020 Feb 4;11(1). pii: mBio.03238-

    Insert

    bfe3
    

    Use

    Bacteroides suicide vector with inducible counter…

    Tags

    signal sequence for periplasmic targeting

    Promoter

    Bacteroides promoter regualted by TetR transcript…

    Available Since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAF123

    Plasmid

    #135921

    Purpose

    All-in-one Sleeping Beauty vector for stable transfection of eukaryotic cells with hygromycin restistance. CMV protomor drives SB100X transposase for transposon integration.

    Depositor

    Andrew S. Flies

    Article

    Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab

    Type

    Empty backbone

    Use

    Transposon

    Expression

    Mammalian

    Available Since

    Jan. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCFJ150-mCherry(dpiRNA)::ANI-1(AHPH)

    Plasmid

    #107939

    Purpose

    pie-1p::mCherry(dpiRNA)::ANI-1(AHPH)::pie-1 3'UTR construct used for MosSCI in nematode. mCherry is optimized by removing all piRNA targeting sites to allow germline expression. RhoA biosenser.

    Depositor

    Heng-Chi Lee

    Article

    Zhang et al Science. 2018 Feb 2;359(6375):587-592. d

    Insert

    mCherry(dpiRNA)::ANI-1(AHPH)
    

    Expression

    Worm

    Promoter

    pie-1

    Available Since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCin4-3xFLAG-MKL1

    Plasmid

    #19838

    Depositor

    Ron Prywes

    Article

    Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

    Insert

    MKL1 (MRTFA Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    Dec. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA

    Plasmid

    #50943

    Purpose

    Bicistronic vector expressing mRuby2 and GCaMP6f from a single open reading frame.

    Depositor

    Tobias Rose, Tobias Bonhoeffer, Mark Huebener

    Article

    Rose et al Science. 2016 Jun 10;352(6291):1319-22.

    Insert

    mRuby2-P2A-GCaMP6f
    

    Use

    AAV

    Promoter

    hSyn1

    Available Since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pWPT-/GCCACC-mEGFP-IRES-mCherry

    Plasmid

    #49235

    Purpose

    Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

    Depositor

    Clifford Wang

    Article

    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

    Inserts

    mEGFP
    mCherry
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    SfuI site to create C-terminal fusions

    Expression

    Mammalian

    Promoter

    EF1-short

    Available Since

    Dec. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge