We narrowed to 61,844 results for: SAP
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Plasmid#132373Purposecontains the sequence of TSapphire for C-term fusions in the pGGD000 backbone (GreenGate)DepositorInsertlinker-TSapphire
UseGreengate entry plasmidAvailable SinceOct. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
Ad-EEF1D-S133E
Plasmid#131577PurposeExpresses EEF1D with S133E mutation in mammalian cellsDepositorInsertEukaryotic translation elongation factor 1 delta (EEF1D Human)
UseAdenoviralTagsNoMutationchanged Serine 133 to Glutamic acidPromoterCMVAvailable SinceOct. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pCDNA3.1-V5-SPR
Plasmid#127236PurposeEukaryotic expression of SPRDepositorAvailable SinceJune 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pE2F3-donor
Plasmid#113809PurposeCRISPR donor plasmid to tag human transcription factor E2F3 with GFPDepositorAvailable SinceApril 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide2
Plasmid#118159PurposeCRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA2 against the EGFP CDSDepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianPromoterEF1a core and U6Available SinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide3
Plasmid#118160PurposeCRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA3 against the EGFP CDSDepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianPromoterEF1a core and U6Available SinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOZ-FH-C Bach1 K52R
Plasmid#73645PurposeRetroviral expression of Bach1 K52Rmutant (helicase null)DepositorInsertBach1 (BACH1 Human)
UseRetroviralTagsFLAG and HisExpressionMammalianMutationK52R (helicase null)PromoterunknownAvailable SinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSMAD3.1.0-gDNA
Plasmid#112407PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor SMAD3DepositorAvailable SinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pELF4-donor
Plasmid#112371PurposeCRISPR donor plasmid to tag human transcription factor ELF4 with GFPDepositorInsertELF4 homology arms flanking EGFP-IRES-Neo cassette (ELF4 Human)
UseCRISPRAvailable SinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVEZF1-donor
Plasmid#112341PurposeCRISPR donor plasmid to tag human transcription factor VEZF1 with GFPDepositorInsertVEZF1 homology arms flanking EGFP-IRES-Neo cassette (VEZF1 Human)
UseCRISPRMutationrs999907002, hg38:17:57975308:A>T, hg38:17:579…Available SinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNR1D2-donor
Plasmid#112314PurposeCRISPR donor plasmid to tag human transcription factor NR1D2 with GFPDepositorInsertNR1D2 homology arms flanking EGFP-IRES-Neo cassette (NR1D2 Human)
UseCRISPRMutationhg38:3:23977483:A>T, hg38:3:23977515:A>G, h…Available SinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
phSOD1-L126X-N’His
Plasmid#117707Purposemutation on stability/solubility of SOD1DepositorInsertSOD1 (SOD1 Human)
TagsHis tag with Thrombin cleavage siteExpressionBacterialMutationL126XPromoterT7Available SinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
phSOD1-D101G-N’His
Plasmid#117704Purposemutation on stability/solubility of SOD1DepositorInsertSOD1 (SOD1 Human)
TagsHis tag with Thrombin cleavage siteExpressionBacterialMutationD101GPromoterT7Available SinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHBS1201 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 6xΦ]
Plasmid#107846PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
ExpressionMammalianMutationAll hydrophobics combined in six clusters in spli…Available SinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHBS1109 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 ∆CTD]
Plasmid#107841PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
ExpressionMammalianMutationDeletion of C-terminal domain in splicing reporterAvailable SinceApril 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHBS1108 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 ∆RRM]
Plasmid#107840PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
ExpressionMammalianMutationDeletion of RRM domains in splicing reporterAvailable SinceApril 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 MAGI1 WW domain #1
Plasmid#104242PurposeBacterial expression of WW domain #1 from MAGI1DepositorInsertMAGI1 WW domain #1 (MAGI1 Human)
TagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable SinceMarch 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 MAGI1 WW domain #2
Plasmid#104243PurposeBacterial expression of WW domain #2 from MAGI1DepositorInsertMAGI1 WW domain #2 (MAGI1 Human)
TagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable SinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only