We narrowed to 5,620 results for: Feng Zhang

1. pLV-dt/deltaEGFP

   Plasmid

   #163919

   Purpose

   PGK-driven dTomato expression. Lacks the CBA-promoter controlling EGFP expression.

   Depositor

   John Engelhardt

   Article

   Choi et al Genes (Basel). 2020 Oct 6;11(10). pii: g

   Insert

   dtomato
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-PGK-dt/CBA-EGFP-NLS

   Plasmid

   #163918

   Purpose

   Contains bidirectional reporter genes dtomato and nuclear-targeting EGFP.

   Depositor

   John Engelhardt

   Article

   Choi et al Genes (Basel). 2020 Oct 6;11(10). pii: g

   Insert

   dtomato and EGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Added nuclear localization signal to EGFP

   Promoter

   Available since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTripZ-B2

   Plasmid

   #163924

   Purpose

   For making a cell line that expresses doxycyline-inducible RNAi suppressor protein B2 for increasing titer of lentivirus with bidirectional promoters during virus production.

   Depositor

   John Engelhardt

   Article

   Choi et al Genes (Basel). 2020 Oct 6;11(10). pii: g

   Insert

   Flock house virus B2 protein (FHVs1gp2 )
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBoBi-hTRPV4-C-GC6s

   Plasmid

   #154150

   Purpose

   To detect the calcium ion releases from TRPV4 channel

   Depositor

   Sheng-cai Lin

   Article

   Li et al Cell Metab. 2019 Sep 3;30(3):508-524.e12

   Insert

   TRPV4 and GCamp6s (TRPV4 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   CMV

   Available since

   Sept. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-sgRNA

   Plasmid

   #71409

   Purpose

   There is no gene/insert. It is a backbone plasmid that others can use to insert sgRNAs of interest. The cloning site for this BsmBI

   Depositor

   David Sabatini, Eric Lander

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. lenti.Cas9.BFP.sgRNA.parental

   Plasmid

   #196715

   Purpose

   Sortable WT-SpCas9 expression and guide cassette (1-vector system).

   Depositor

   Bernhard Schmierer

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Mutation

   Promoter

   Available since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. PP7 guide

   Plasmid

   #86168

   Purpose

   Expresses S. Pyogenes guide with PP7 aptamer in mammalian cells

   Depositor

   Amit Choudhary

   Article

   Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

   Insert

   PP7 guide
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. lentiGuide-Puro-Chen-tracrRNA_2xBsmBI

   Plasmid

   #196710

   Purpose

   Backbone for cloning single guides (Chen tracrRNA PMID: 24360272) or guide libraries with untranscribed barcodes and a tracrRNA of choice

   Depositor

   Bernhard Schmierer

   Article

   Schmierer et al Mol Syst Biol. 2017 Oct 9;13(10):945. do

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. AiP942-pX330-Sncg

   Plasmid

   #181774

   Purpose

   CRISPR/Cas9 guide vector

   Depositor

   Tanya Daigle

   Insert

   Sncg guide
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. AiP926-pX330-Batf3

    Plasmid

    #181772

    Purpose

    CRISPR/Cas9 guide vector

    Depositor

    Tanya Daigle

    Insert

    Batf3 guide
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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