We narrowed to 28,450 results for: tat

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TUBE1 F5.4 gRNA

Plasmid

#90928

Purpose

3rd generation lentiviral gRNA plasmid targeting human TUBE1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

TUBE1 (Guide Designation F5.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pGL2-MCT2Prom2.5

Plasmid

#87351

Purpose

Human monocarboxylate transporter 2 (MCT2) promoter 2.5 kbp

Depositor

Saroj Mathupala

Article

Caruso et al Neoplasia. 2017 Jan 13;19(2):121-134. do

Insert

MCT2 (SLC16A7 Human)

Use

Luciferase

Expression

Bacterial and Mammalian

Promoter

2.5 kbp proximal promoter of human MCT2 (nested d…

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pGL2-MCT2Prom1.1

Plasmid

#87352

Purpose

Human monocarboxylate transporter 2 (MCT2) promoter 1.1 kbp

Depositor

Saroj Mathupala

Article

Caruso et al Neoplasia. 2017 Jan 13;19(2):121-134. do

Insert

MCT2 (SLC16A7 Human)

Use

Luciferase

Expression

Bacterial and Mammalian

Promoter

1.1 kbp proximal promoter of human MCT2 (nested d…

Available Since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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pGL2-MCT2Prom3.2

Plasmid

#87349

Purpose

Human monocarboxylate transporter 2 (MCT2) promoter 3.2 kbp

Depositor

Saroj Mathupala

Article

Caruso et al Neoplasia. 2017 Jan 13;19(2):121-134. do

Insert

MCT2 (SLC16A7 Human)

Use

Luciferase

Expression

Bacterial and Mammalian

Promoter

3.2 kbp proximal promoter of MCT2 (nested deletio…

Available Since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCIneoRL-BCL2 3'UTR-mir200c-mut

Plasmid

#84598

Purpose

Renilla Luciferase reporter assay for BCL2 3'UTR with mutations on miR-200c binding site

Depositor

Yanan Yang

Article

Zhang et al Nat Commun. 2016 Jul 26;7:12231. doi: 10

Insert

BCL2 3'UTR (UTS2R Human)

Use

Luciferase

Tags

Renilla Luciferase

Mutation

Mutation on miR-200c binding site on BCL2 3'…

Promoter

CMV

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only
pCIneoRL-TOB1 3'UTR-mir200c-mut

Plasmid

#84590

Purpose

Renilla Luciferase reporter assay for TOB1 3'UTR with mutations on miR-200c binding site

Depositor

Yanan Yang

Article

Zhang et al Nat Commun. 2016 Jul 26;7:12231. doi: 10

Insert

TOB1 3'UTR (UTS2R Human)

Use

Luciferase

Tags

Renilla Luciferase

Mutation

Mutation on miR-200c binding site on TOB1 3'…

Promoter

CMV

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only
pCIneoRL-RanBP93'UTR-mir200c-mut

Plasmid

#84592

Purpose

Renilla Luciferase reporter assay for RANBP9 3'UTR with mutations on miR-200c binding site

Depositor

Yanan Yang

Article

Zhang et al Nat Commun. 2016 Jul 26;7:12231. doi: 10

Insert

RANBP9 3'UTR (RANBP9 Human)

Use

Luciferase

Tags

Renilla Luciferase

Mutation

Mutation on miR-200c binding site on RANBP9 3…

Promoter

CMV

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only
pVH003

Plasmid

#80397

Purpose

Contains pBAD-CusR (response regulator) and pCusR-antiscaffold-YFP-AAV. This plasmid was used for microscopy tracking experiments

Depositor

Richard Murray

Article

Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.

Inserts

Anti-scaffold - Venus YFP
CusR (cusR )
AraC (araC )

Use

Synthetic Biology

Tags

Fused by GS linker to LZx domain and Venus has N-…

Expression

Bacterial

Available Since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-Mbd3b-pA-pgk-bsd

Plasmid

#74920

Purpose

Mammalian expression of Mbd3b

Depositor

Jose Silva

Article

dos Santos et al Cell Stem Cell. 2014 Jul 3;15(1):102-10.

Insert

Mnd3b (Mbd3 Mouse)

Expression

Mammalian

Promoter

CAG

Available Since

May 17, 2016

Availability

Academic Institutions and Nonprofits only
pPB-CAG-Mbd3c-pA-pgk-bsd

Plasmid

#74921

Purpose

Mammalian expression of Mbd3c

Depositor

Jose Silva

Article

dos Santos et al Cell Stem Cell. 2014 Jul 3;15(1):102-10.

Insert

Mbd3c (Mbd3 Mouse)

Expression

Mammalian

Promoter

CAG

Available Since

May 17, 2016

Availability

Academic Institutions and Nonprofits only
pES006 (pTet-qacR 2/pQacA-deGFP)

Plasmid

#69035

Purpose

reporter with qacR 2 on single plasmid

Depositor

Richard Murray

Article

de Los Santos et al ACS Synth Biol. 2015 Aug 19.

Inserts

qacR (qacR Synthetic)
deGFP

Tags

His6 tag

Expression

Bacterial

Mutation

E57Q, E58L, W61Y, E90Q, I99Q, M116Q, L119Y, E120Q…

Promoter

pQacA and pTet

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pET151-hUCH37 (ISF1)(M148D/F149D)

Plasmid

#71388

Purpose

Expression of hUCH37 (M148D/F149D) in E.coli

Depositor

Chris Hill

Article

VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

Insert

hUCH37 (ISF1) M148D/F149D (UCHL5 Human)

Tags

HIS-TEV sequence

Expression

Bacterial

Mutation

changed methionine 148 to aspartate, phenylalanin…

Promoter

T7

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only
Hy_pMT Laccase2 MCS-Laccase2 608-1097

Plasmid

#69890

Purpose

Expresses the Drosophila laccase2 exon 2 circular RNA

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

Laccase2 (stw Fly)

Expression

Insect

Mutation

L158I in laccase2

Promoter

Metallothionein Promoter (pMT)

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pET30Xa-SBL1(160E)

Plasmid

#66846

Purpose

Expresses soybean lipoxygenase-1 S160E mutant with N-term Histag

Depositor

Betty Gaffney

Article

Coffa et al J Biol Chem. 2005 Nov 18;280(46):38756-6

Insert

soybean lipoxygenase-1 (LOC547694, NEWENTRY Glycine max)

Tags

His-tag

Expression

Bacterial

Mutation

S160E

Promoter

T7lac

Available Since

Sept. 16, 2015

Availability

Academic Institutions and Nonprofits only

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CFP(159-238) in pcDNAI/Amp

Plasmid

#60896

Purpose

This vector attaches CFP(159-238) to the N-terminus of a protein.

Depositor

Catherine Berlot

Article

Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E

Insert

CFP(159-238)

Expression

Mammalian

Mutation

Asn-164 to His

Promoter

CMV

Available Since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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pFRT-TODestFLAGHA_RBPMS_KDR_E97A_K100A

Plasmid

#65660

Purpose

FLAG-HA expression vector with RBPMS carrying mutation E97A, K100A and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)

Depositor

Thomas Tuschl

Article

Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

Insert

RBPMS (RBPMS Human)

Tags

FLAG HA

Expression

Mammalian

Mutation

changed glutamic acid 97 to alanine, lysine 100 t…

Promoter

CMV, 2x Tet

Available Since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-hRIP1-FL I541P

Plasmid

#61381

Purpose

Expresses Human full length RIPK1 with I541P mutation in the mammalian cells

Depositor

Francis Chan

Article

Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

Insert

Full length human RIP1with I541P mutation (RIPK1 Human)

Tags

EGFP and HA tag

Expression

Mammalian

Mutation

changed I541 to P

Available Since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
pSicoR-ATF7IPi1

Plasmid

#59614

Purpose

Expression of shRNA against human ATF7IP

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

ATF7IP (ATF7IP Human)

Use

RNAi

Expression

Mammalian

Promoter

U6

Available Since

Oct. 1, 2014

Availability

Academic Institutions and Nonprofits only
pBABEpuro-ARAF_D429A

Plasmid

#51080

Purpose

mammalian expression of ARAF D429A

Depositor

Matthew Meyerson

Article

Imielinski et al J Clin Invest. 2014 Apr;124(4):1582-6. d

Insert

ARAF (ARAF Human)

Use

Retroviral

Expression

Mammalian

Mutation

D429A

Available Since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

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1287 p3E-mut let-7 etv2 3’ UTR

Plasmid

#49011

Purpose

Zebrafish Long length etv2 3' UTR with five let7 binding site mutations in gateway 3' Entry vector

Depositor

Nathan Lawson

Article

Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

Insert

ets variant gene 2 (etsrp Zebrafish)

Use

Gateway 3' entry

Mutation

Mutated the Let7 miRNA binding sites

Available Since

Nov. 7, 2013

Availability

Academic Institutions and Nonprofits only