We narrowed to 11,617 results for: 110

1. pHL 1405

   Plasmid

   #53010

   Purpose

   pLtetO-1:RhyB, pCon:DsrA, pLtetO-1:hfq, pLlacO-1:sodB::gfp

   Depositor

   Han Lim

   Article

   Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

   Inserts

   RhyB sRNA
   DsrA sRNA
   hfq
   sodB
   

   Use

   Synthetic Biology

   Tags

   gfp

   Expression

   Bacterial

   Mutation

   contains -56 to +141 relative to start codon

   Promoter

   pCon, pLlacO-1, and pLtetO-1

   Available Since

   May 16, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pHL 1032

   Plasmid

   #52997

   Purpose

   pLtetO-1:RhyB, pCon:ompC::mCherry, pLlacO-1:sodB::gfp

   Depositor

   Han Lim

   Article

   Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

   Inserts

   RhyB sRNA
   ompC
   sodB
   

   Use

   Synthetic Biology

   Tags

   gfp and mCherry

   Expression

   Bacterial

   Mutation

   contains −56 to +141 bp relative to start codon a…

   Promoter

   pCon, pLlacO-1, and pLtetO-1

   Available Since

   May 16, 2014

   Availability

   Academic Institutions and Nonprofits only

3. Intron-Tagging-EGFP-Donor

   Plasmid

   #159740

   Purpose

   Contains EGFP flanked by a splice acceptor and a splice donor. Together with other intron tagging plasmids, it can be used to place the EGFP tag as a synthetic exon into introns of target genes.

   Depositor

   Stefan Kubicek

   Article

   Reicher et al Genome Res. 2020 Dec;30(12):1846-1855. d

   Insert

   EGFP
   

   Use

   Intron tagging donor

   Available Since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pK.myc-MYPT1

   Plasmid

   #24101

   Depositor

   Masumi Eto

   Article

   Eto et al Cell Motil Cytoskeleton. 2005 Oct . 62(2

   Insert

   Myosin-targeting subunit-1 (PPP1R12A Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   S50G, P415Q

   Available Since

   March 19, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDIRECT_10A

   Plasmid

   #91207

   Purpose

   Direct Cloning, Type: non-T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: none

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   35S:AtCas9 + AtU6:gRNA
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti_rtTA-P2A-BFP_HygroR

   Plasmid

   #186738

   Purpose

   rtTA-P2A-BFP expression construct cloned into lentiviral backbone

   Depositor

   Richard Sherwood

   Article

   Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

   Insert

   rtTA
   

   Use

   Lentiviral

   Tags

   P2A-BFP

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMC_mNG2(11)_BSDminus_F2

   Plasmid

   #184164

   Purpose

   Donor cassette plasmid for high-throughput tagging, encoding an mNG2(11) synthetic exon in frame 2, as well as a blasticidin resistance gene for selection of genomic integrants.

   Depositor

   Ophir Shalem

   Article

   Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

   Inserts

   mNG2(11)
   bsd
   

   Use

   CRISPR

   Available Since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAT.000

   Plasmid

   #119559

   Purpose

   Self replication Level T acceptor vector with LacZ compatible blue/white screening. Modified BioBrick vector, RSF1010-derived ori.

   Depositor

   Alistair McCormick

   Article

   Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hSynapsin1-FLEx-axon-jGCaMP8m-WPRE-SV40

   Plasmid

   #216533

   Purpose

   An axon-targeted jGCaMP8m controlled by hSynapsin1 promoter and flex switch

   Depositor

   Yuki Kambe

   Article

   Abe et al bioRxiv. 2023 Aug 24:2023.08.23.554475.

   Insert

   axon-jGCaMP8m
   

   Use

   AAV and Cre/Lox

   Tags

   GAP43

   Expression

   Mammalian

   Promoter

   hSynapsin1

   Available Since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. Stat3 Flag pRc/CMV

    Plasmid

    #8707

    Depositor

    Jim Darnell

    Article

    Horvath et al Genes Dev 1995 Apr 15;9(8):984-94.

    Insert

    Stat3 (Stat3 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    *see comments

    Available Since

    Sept. 26, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDULE-ABK

    Plasmid

    #49086

    Purpose

    A pyrrolysyl tRNA/tRNA sythetase pair evolved to efficiently incorporate an aliphatic diazirine amino acid into proteins in E. coli and mammalian cells for crosslinking experiments

    Depositor

    Peter Schultz

    Article

    Ai et al Chembiochem. 2011 Aug 16;12(12):1854-7.

    Inserts

    tRNA synthetase
    tRNA for a photocrosslinking amino acid
    

    Expression

    Bacterial

    Mutation

    L274M, C313A, Y349F

    Promoter

    GlnRS and lpp

    Available Since

    Nov. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. qs5

    Plasmid

    #24498

    Depositor

    Bruce Conklin

    Article

    Conklin et al Mol Pharmacol. 1996 Oct . 50(4):885-90.

    Insert

    Gq alpha (Gnaq Mouse)
    

    Tags

    HA (see comments)

    Expression

    Mammalian

    Mutation

    Replacement of the five carboxyl-terminal amino a…

    Available Since

    June 1, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pET15TV-L-hlPYK WT

    Plasmid

    #187975

    Purpose

    Expresses the human liver pyruvate kinase

    Depositor

    Audrey Lamb

    Article

    McFarlane et al Acta Crystallogr F Struct Biol Commun. 2

    Insert

    hlPYK WT
    

    Tags

    Hexahistag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pETM33_SUFU_Sufu

    Plasmid

    #178488

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    SUFU_Sufu (SUFU Synthetic, Human)
    

    Tags

    His-GST

    Expression

    Bacterial

    Promoter

    T7/LacO

    Available Since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMSCV-BLU-VIPR

    Plasmid

    #220497

    Purpose

    Retroviral plasmid allowing for potent induction of gRNA using blue light. The plasmid contains VPR-EL222-2A-Thy1.1 and the C120 element followed by cloning sites for ribozyme flanked gRNA (RGR).

    Depositor

    Alexander Espinosa

    Article

    Pulgarin et al bioRxiv [Preprint]. 2023 Nov 10:2023.11.

    Type

    Empty backbone

    Use

    CRISPR and Retroviral

    Expression

    Mammalian

    Available Since

    Aug. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBLU-VIPR

    Plasmid

    #220498

    Purpose

    Plasmid allowing for potent induction of gRNA using blue light. The plasmid contains VPR-EL222 and the C120 response element followed by mCherry and cloning sites for ribozyme flanked gRNA (RGR).

    Depositor

    Alexander Espinosa

    Article

    Pulgarin et al bioRxiv [Preprint]. 2023 Nov 10:2023.11.

    Type

    Empty backbone

    Use

    CRISPR; Transposon

    Expression

    Mammalian

    Available Since

    Aug. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. mCherry-CRY2-iSH2

    Plasmid

    #66839

    Purpose

    Expression of a light-inducible PI3-kinase, generated by fusing the iSH2 domain of human p85α (residues 420–615) to mCherry-CRY2. Binds endogenous PI3-kinase p110α catalytic subunits constitutively.

    Depositor

    Pietro De Camilli

    Article

    Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

    Insert

    iSH2
    

    Tags

    mCherry-CRY2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pETM33_SIN3A_PAH

    Plasmid

    #178486

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    SIN3A_PAH (SIN3A Synthetic, Human)
    

    Tags

    His-GST

    Expression

    Bacterial

    Promoter

    T7/LacO

    Available Since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDIRECT_25F

    Plasmid

    #91143

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9 + TaU6:gRNA, Plant Selection: PvUbi2:hpt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: ZmUbi:TaCas9 + TaU6:gRNA
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. G10 Cas9 MDC123

    Plasmid

    #59187

    Purpose

    G10 promoting Glycing Max codon optimized Cas9 (N and C terminus NLS) with bar plant selection

    Depositor

    Robert Stupar

    Article

    Michno et al GM Crops Food. 2015;6(4):243-52. doi: 10

    Insert

    Glycine Max codon optimized Cas9
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    G10

    Available Since

    Dec. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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