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NPM1-TurboID-MSCV-IRES-GFP

Plasmid

#240134

Purpose

Expresses NPM1-TurboID fusion in mammalian cells

Depositor

Timothy Ley

Article

Kramer et al J Clin Invest. 2025 Aug 7:e195929. doi:

Insert

NPM1 (NPM1 Human)

Use

Retroviral

Tags

IRES-GFP and TurboID

Expression

Mammalian

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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Gab2-MSCV-IRES-GFP

Plasmid

#240126

Purpose

Expresses Gab2 (mouse) in mammalian cells

Depositor

Timothy Ley

Article

Kramer et al J Clin Invest. 2025 Aug 7:e195929. doi:

Insert

Gab2 (Gab2 Mouse)

Use

Retroviral

Tags

IRES-GFP

Expression

Mammalian

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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Pak1-MSCV-IRES-GFP

Plasmid

#240127

Purpose

Expresses Pak1 (mouse) in mammalian cells

Depositor

Timothy Ley

Article

Kramer et al J Clin Invest. 2025 Aug 7:e195929. doi:

Insert

Pak1 (Pak1 Mouse)

Use

Retroviral

Tags

IRES-GFP

Expression

Mammalian

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pET-29_GSS-6xHis-SUMO-VidC-EGFP-FLAG

Plasmid

#241009

Purpose

VidC-EGFP construct that can be purified via His-tag and undergo N-terminal conjugation using the VEPL system.

Depositor

Cole DeForest

Article

Gharios et al Bioconjug Chem. 2024 Jun 19;35(6):750-75

Insert

GSS-6xHis-SUMO-VidC-EGFP-FLAG

Tags

FLAG-tag, His-tag, and SUMO-tag

Expression

Bacterial

Promoter

T7

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pET-29_GSS-6xHis-SUMO-VidC-mCherry-FLAG

Plasmid

#241010

Purpose

VidC-mCherry construct that can be purified via His-tag and undergo N-terminal conjugation using the VEPL system.

Depositor

Cole DeForest

Article

Gharios et al Bioconjug Chem. 2024 Jun 19;35(6):750-75

Insert

GSS-6xHis-SUMO-VidC-mCherry-FLAG

Tags

FLAG-tag, His-tag, and SUMO-tag

Expression

Bacterial

Promoter

T7

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pET-21_GSS-6xHis-SUMO-VidC-bla-FLAG

Plasmid

#241012

Purpose

VidC-bla construct that can be purified via His-tag and undergo N-terminal conjugation using the VEPL system.

Depositor

Cole DeForest

Article

Gharios et al Bioconjug Chem. 2024 Jun 19;35(6):750-75

Insert

GSS-6xHis-SUMO-VidC-bla-FLAG

Tags

FLAG-tag, His-tag, and SUMO-tag

Expression

Bacterial

Promoter

T7

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pET-21_GSS-6xHis-SUMO-VidC-EGF-FLAG

Plasmid

#241011

Purpose

VidC-EGF construct that can be purified via His-tag and undergo N-terminal conjugation using the VEPL system.

Depositor

Cole DeForest

Article

Gharios et al Bioconjug Chem. 2024 Jun 19;35(6):750-75

Insert

GSS-6xHis-SUMO-VidC-EGF-FLAG

Tags

FLAG-tag, His-tag, and SUMO-tag

Expression

Bacterial

Promoter

T7

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pVT-LUC

Plasmid

#234915

Purpose

This all-in-one vector is used to express the LUC gene

Depositor

Guoli Song

Article

Hao et al Plant Commun. 2025 May 19:101379. doi: 1

Insert

LUC

Expression

Plant

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pVT-EGFP

Plasmid

#234917

Purpose

This all-in-one vector is used to express the EGFP gene

Depositor

Guoli Song

Article

Hao et al Plant Commun. 2025 May 19:101379. doi: 1

Insert

EGFP

Expression

Plant

Available Since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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pVTHV3

Plasmid

#234924

Purpose

All-in-one bimolecular fluorescence complementation (BiFC) vector

Depositor

Guoli Song

Article

Hao et al Plant Commun. 2025 May 19:101379. doi: 1

Type

Empty backbone

Expression

Plant

Available Since

June 16, 2025

Availability

Academic Institutions and Nonprofits only

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pVTc

Plasmid

#234913

Purpose

All-in-one plant virus vector to detect circular genomes.

Depositor

Guoli Song

Article

Hao et al Plant Commun. 2025 May 19:101379. doi: 1

Type

Empty backbone

Expression

Plant

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyso-InPTapp

Plasmid

#236000

Purpose

Genetically encoded FRET-based phosphoinositide sensor for monitoring PI(3,4)P2. Targeted to lysosome surface.

Depositor

Jin Zhang

Article

Sahan et al Proc Natl Acad Sci U S A. 2025 Apr;122(1

Insert

Lyso-InPTapp (PLEKHA1, LAMP1 Human, Synthetic)

Tags

Citrine, Full-length LAMP1, and truncECFP

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyso-InPGrp

Plasmid

#236002

Purpose

Genetically encoded FRET-based phosphoinositide sensor for monitoring PI(3,4,5)P3. Targeted to lysosome surface.

Depositor

Jin Zhang

Article

Sahan et al Proc Natl Acad Sci U S A. 2025 Apr;122(1

Insert

Lyso-InPGrp (Cyth3 Synthetic, Rat)

Tags

Cerulean, Full-length LAMP1, and cpVenus[E172]

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT376: pTwinStrep-bdSUMO-ZooTsy_TnsF-Y584F

Plasmid

#200877

Purpose

ZooTsy_TnsF expression construct for protein purification.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

ZooTsy_TnsF

Expression

Bacterial

Mutation

TnsF-Y584F

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT221: pR6K-Donor(ZooTsy)_H1:12_E1:135_E2:20_H2:12

Plasmid

#200874

Purpose

ZooTsy donor harboring KanR cargo.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

ZooTsy

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT384: pSC101-Donor(ZooTsy)

Plasmid

#200875

Purpose

ZooTsy donor harboring KanR cargo.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

ZooTsy

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT81: pTarget(ZooTsy)

Plasmid

#200876

Purpose

Target plasmid containing attachement site for ZooTsy.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

ZooTsy_comM

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT315: pHelper(ZooTsy)_TnsF-Y584F

Plasmid

#200872

Purpose

Inducible expression of ZooTsy proteins.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

ZooTsy

Expression

Bacterial

Mutation

TnsF-Y584F

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT155: pHelper(ZooTsy)_ΔHTH

Plasmid

#200868

Purpose

Inducible expression of ZooTsy (except HTH) proteins.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

ZooTsy

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT156: pHelper(ZooTsy)_ΔTnsF

Plasmid

#200869

Purpose

Inducible expression of ZooTsy (except TnsF) proteins.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

ZooTsy

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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