We narrowed to 42,759 results for: Ina

1. pCMV-hAR-L26A,F27A,W433A,L436A,F437A

   Plasmid

   #89085

   Purpose

   mammalian expression of human androgen receptor with mutations of N-terminal FxxLF and WxxLF motifs

   Depositor

   Elizabeth Wilson

   Article

   He et al J Biol Chem. 2000 Jul 28;275(30):22986-9

   Insert

   hAR FxxLF WxxLF mutant (AR Human)
   

   Expression

   Mammalian

   Mutation

   hAR-L26A,F27A,W433A,L436A,F437A

   Promoter

   CMV

   Available Since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

2. pDEST-mCherry-GJA1-20k-N1

   Plasmid

   #49861

   Purpose

   Encodes C-terminal fragment of human connexin 43 initiating at M213 with a V5 C-terminal fusion tag

   Depositor

   Robin Shaw

   Article

   Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

   Insert

   GJA1-20k (GJA1 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   GJA1-20k corresponds to residues 213 to 382 to Co…

   Promoter

   CMV

   Available Since

   Dec. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-DYRK1A-2A-mCherry

   Plasmid

   #118272

   Purpose

   Expresses mouse DYRK1A tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells.

   Depositor

   Vance Lemmon

   Article

   Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5

   Insert

   dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1a (Dyrk1a Mouse)
   

   Use

   AAV

   Tags

   2A peptide and mCherry

   Expression

   Mammalian

   Promoter

   CMV with beta global intron

   Available Since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFB-EPCP5

   Plasmid

   #228879

   Purpose

   Expression of Z-tagged LRRK2 KW using Baculoviruses

   Depositor

   Sebastian Mathea

   Article

   Raig et al bioRxiv [Preprint]. 2025 Feb 13:2024.09.

   Insert

   Leucine-rich repeat kinase 2 (LRRK2 Human)
   

   Tags

   His-Z-TEV

   Expression

   Insect

   Mutation

   Amino acids deleted 1-1846

   Available Since

   Jan. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-Tag4A-MMP8 Wt

   Plasmid

   #29545

   Depositor

   Yardena Samuels

   Article

   Palavalli et al Nat Genet. 2009 May . 41(5):518-20.

   Insert

   Metalloproteinase protein 8 (MMP8 Human)
   

   Tags

   FLAG

   Expression

   Bacterial and Mammalian

   Available Since

   May 23, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pLKO_Ollas.S.V5_mCherry-NLS

   Plasmid

   #178211

   Purpose

   Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

   Depositor

   Brian Brown

   Article

   Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

   Insert

   nPC Tagged mCherry-NLS
   

   Use

   Lentiviral

   Tags

   Nuclear Localization Signal and Ollas.S.V5

   Promoter

   eF1a

   Available Since

   July 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. K785R Smarca4-V5 IRES-puro

   Plasmid

   #107059

   Purpose

   Expression of dominant-negative K785R Smarca4 (Brg1)-V5 fusion with IRES puromycin resistance.

   Depositor

   Jerry Crabtree, Courtney Hodges

   Article

   Hodges et al Nat Struct Mol Biol. 2018 Jan;25(1):61-7

   Insert

   SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
   

   Use

   Lentiviral

   Tags

   IRES-puroR and V5

   Expression

   Mammalian

   Mutation

   K785R

   Promoter

   CAG

   Available Since

   April 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lenti-pNFkB::Cre-mRuby3

   Plasmid

   #164137

   Purpose

   Lentiviral construct for building stable cell lines expressing Cre recombinase and mRuby3 in the presence of TNFa

   Depositor

   Hyongbum Kim

   Article

   Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

   Insert

   NFkB promoter-Cre-T2A-mRuby3
   

   Use

   CRISPR, Cre/Lox, Lentiviral, and Synthetic Biolog…

   Expression

   Mammalian

   Promoter

   NFkB synthetic promoter

   Available Since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLVX-CLK1

   Plasmid

   #174088

   Purpose

   Inducibly expresses Myc-CLK1 in mammalian cells with the Tet-on system

   Depositor

   Wesley Sundquist, Katie Ullman

   Article

   Williams et al Elife. 2021 Aug 4;10. pii: 63743. doi: 1

   Insert

   CLK1 (CLK1 Human)
   

   Use

   Lentiviral

   Tags

   Myc-GSSS

   Expression

   Mammalian

   Promoter

   TREtight

   Available Since

   Sept. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. Plxnd1-Fc-His

    Plasmid

    #72132

    Purpose

    Expresses the extracellular region of the PlexinD1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Plxnd1 (Plxnd1 Mouse)
    

    Tags

    Fc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lenti-pHSE::Cre-mRuby3

    Plasmid

    #164136

    Purpose

    Lentiviral construct for building stable cell lines expressing Cre recombinase and mRuby3 in the presence of heat induction

    Depositor

    Hyongbum Kim

    Article

    Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

    Insert

    HSE promoter-Cre-T2A-mRuby3
    

    Use

    CRISPR, Cre/Lox, Lentiviral, and Synthetic Biolog…

    Expression

    Mammalian

    Promoter

    HSE synthetic promoter

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPSV39-dddAI

    Plasmid

    #186557

    Purpose

    Broad host range regulated expression of the DddA immunity determinant (DddAI)

    Depositor

    Joseph Mougous

    Article

    Gallagher et al Nat Microbiol. 2022 Jun;7(6):844-855. do

    Insert

    dddAI
    

    Expression

    Bacterial

    Promoter

    lacUV5

    Available Since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA-GalNAc-T2-GFP-ESCargo(FTV)

    Plasmid

    #140163

    Purpose

    Expresses a regulatable secretory cargo for mammalian cells and a Golgi marker

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    GalNAc-T2-msGFP2 and piGH-FTVNTT-DsRed-Express2-FKBP(LV)(C22V) (GALNT2 Human, Synthetic)
    

    Tags

    ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. L1cam-Fc-His

    Plasmid

    #72083

    Purpose

    Expresses the extracellular region of the L1CAM protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    L1cam (L1cam Mouse)
    

    Tags

    Fc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLAP-BUBR1

    Plasmid

    #114052

    Purpose

    transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

    Depositor

    Geert Kops

    Article

    Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

    Insert

    BUBR1 (BUB1B Human)
    

    Tags

    YFP-TEV-S- Human LAP

    Expression

    Mammalian

    Mutation

    C2823A and G2826A (siRNA-resistance)

    Available Since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCOTS-pyl-GFP(35TAG)

    Plasmid

    #92047

    Purpose

    This is a S. elongatus (PCC7942) cyanobacterial plasmid that encodes the pylRS orthogonal translation system. In addition it encodes for a GFP reporter with TAG mutation at site 35.

    Depositor

    Lital Alfonta

    Article

    Chemla et al Biochemistry. 2017 Apr 12. doi: 10.1021/

    Inserts

    EGFP
    Pyrrolysyl tRNA(cua)
    Pyrrolysyl tRNA synthetase (methanosarcina mazei)
    

    Use

    Replicative expression plasmid for cyanobacteria …

    Tags

    Histag

    Mutation

    Tyrosine in position 35 of the GFP was mutated f…

    Promoter

    LeuP (native S. elongatus promoter), PpsbII, and …

    Available Since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP-hLC3

    Plasmid

    #87872

    Purpose

    Express a EGFP-human MAP1-LC3 in mammalian cells

    Depositor

    Eiki Kominami, Isei Tanida

    Article

    Tanida et al J Biol Chem. 2001 Jan 19;276(3):1701-6.

    Insert

    MAP1LC3 (MAP1LC3B Human)
    

    Expression

    Mammalian

    Available Since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. myr-FLAG-PIK3CA-pcw107

    Plasmid

    #64605

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    David Sabatini, Kris Wood

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    PIK3CA (PIK3CA Bovine)
    

    Use

    Lentiviral

    Tags

    FLAG and myristoylated

    Promoter

    PGK

    Available Since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pGEX6P1-human full-length LIC1

    Plasmid

    #74597

    Purpose

    used for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag

    Depositor

    Ron Vale

    Article

    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

    Insert

    full length human dynein light intermediate chain 1 (DYNC1LI1 Human)
    

    Tags

    N-terminal GST and Strep II

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEMHE-mScarlet-hCenpC

    Plasmid

    #171490

    Purpose

    T7 promotor drives in vitro transcription of mScarlet-tagged human hCenpC mRNA

    Depositor

    Melina Schuh

    Article

    Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

    Insert

    CenpC (CENPC Human)
    

    Use

    Pgehme

    Tags

    mScarlet

    Promoter

    T7

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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