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pLenti CMV Puro DEST (w118-1) RALB

Plasmid

#107506

Purpose

Expressing RALB, puromycin resistant

Depositor

Alejandro Gutierrez

Publication

Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

Insert

RALB (RALB Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Puro DEST (w118-1) CRIP2

Plasmid

#107509

Purpose

Expressing CRIP2, puromycin resistant

Depositor

Alejandro Gutierrez

Publication

Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

Insert

CRIP2 (codon optimized) (CRIP2 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Codon optimized sequence

Promoter

CMV

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynap-FLEX.SF-iGluSnFR.S72A

Plasmid

#106182

Purpose

Weak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Publication

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.S72A

Use

AAV

Mutation

GltI: S72A

Promoter

hSynapsin

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pUC19_CoV-2_frC

Plasmid

#253184

Purpose

For recombinant virus rescue; encodes fragment C of SARS-CoV-2 (nt15100-20958)

Depositor

Thomas Klimkait

Publication

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2 frC

Expression

Bacterial

Available since

April 27, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD_SV40

Plasmid

#253185

Purpose

For recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867)

Depositor

Thomas Klimkait

Publication

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2 frD

Expression

Bacterial

Available since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frB

Plasmid

#253183

Purpose

For recombinant virus rescue; encodes fragment B of SARS-CoV-2 (nt8590-15107)

Depositor

Thomas Klimkait

Publication

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2 frB

Expression

Bacterial

Available since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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pdCas12f1Cas_ABE-LVA

Plasmid

#220995

Purpose

Prokaryotic and constitutive expression of AsCas12f1/TadA8e-LVA fusion

Depositor

John van der Oost

Publication

Swartjes et al iScience. 2025 Nov 12;28(12):114033. doi

Insert

Acidibacillus sulfuroxidans Cas12f1-ABE

Use

CRISPR

Tags

LVA

Expression

Bacterial

Mutation

D225A

Available since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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SLX4IP-SFB

Plasmid

#247871

Purpose

Mammalian expression construct for C-terminal S protein-Flag-Streptavidin binding peptide (SFB)-tagged SLX4IP

Depositor

Junjie Chen, Huimin Zhang

Publication

Zhang et al Nucleic Acids Res. 2019 Nov 4;47(19):101

Insert

SLX4IP (C20orf94 Human)

Tags

S protein-Flag-Streptavidin binding peptide (SFB)

Expression

Mammalian

Available since

Dec. 12, 2025

Availability

Academic Institutions and Nonprofits only

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SFB-XPF

Plasmid

#247887

Purpose

Mammalian expression construct for N-terminal S protein-Flag-Streptavidin binding peptide (SFB)-tagged XPF

Depositor

Junjie Chen, Huimin Zhang

Publication

Zhang et al Nucleic Acids Res. 2019 Nov 4;47(19):101

Insert

XPF (ERCC4 Human)

Tags

S protein-Flag-Streptavidin binding peptide (SFB)

Expression

Mammalian

Available since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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ED_p_126

Plasmid

#246073

Purpose

Bacterial expression of ECOR31 2TMbeta residues 72-200 with N-terminal His-SUMO tag and co-expression of ECOR31 mCpol

Depositor

Jennifer Doudna

Publication

Doherty et al Nature. 2025 Nov;647(8091):997-1004. doi

Insert

2TMbeta

Expression

Bacterial

Available since

Oct. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pLPC-Myc-BirA

Plasmid

#234039

Purpose

Expresses Myc-His-BirA(R118G)

Depositor

Roderick O'Sullivan

Publication

Garcia-Exposito et al Cell Rep. 2016 Nov 8;17(7):1858-1871. do

Insert

BirA

Use

Retroviral

Tags

Myc-6xHis

Expression

Mammalian

Mutation

R118G

Available since

Aug. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-YFP-Rb-IRES-blasticidin

Plasmid

#223964

Purpose

Fluorescent reporter with exogenous Rb

Depositor

Hee Won Yang

Publication

Kim et al Cell Rep. 2023 Nov 28;42(11):113198. doi

Insert

H2B-iRFP670-p2a-YFP-Rb (RB1, H2B Human)

Use

Lentiviral

Tags

YFP

Expression

Mammalian

Promoter

EF-1a

Available since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pYO395

Plasmid

#235734

Purpose

Expression of human VPS34 complex I in mammalian cells

Depositor

Roger Williams

Publication

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Inserts

ATG14 (ATG14 Human)
EGFP
BECN1 (BECN1 Human)
PIK3C3 (PIK3C3 Human)
PIK3R4 (PIK3R4 Human)

Tags

ZZ-3xTEV

Expression

Mammalian

Mutation

N256S

Promoter

CMV

Available since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pYO1025

Plasmid

#235705

Purpose

Expression of FL human VPS34 and human VPS15-ZZ in mammalian cells

Depositor

Roger Williams

Publication

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Inserts

VPS34 (PIK3C3 Human)
VPS15 (PIK3R4 Human)

Tags

3xTEV-ZZ

Expression

Mammalian

Promoter

CAG and CMV

Available since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pBK_Gal4-DBD-GR(NR3C1)

Plasmid

#227100

Purpose

GR receptor overexpression for luciferase and barcode assays

Depositor

Michael Wehr

Publication

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

GR (NR3C1 Human)

Expression

Mammalian

Available since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pGL4-CMV-3xFLAG-NLS/SV40-AcGFP-NES/PKI-P2A-Puro

Plasmid

#228399

Purpose

Encodes 3xFLAG-NLS/SV40-AcGFP-NES/PKI which is a AcGFP with an N-terminal SV40 NLS and a C-terminal PKI NES that shuttles between the nucleus and the cytoplasm

Depositor

Dirk Daelemans

Publication

Vercruysse et al Commun Biol. 2024 Nov 23;7(1):1560. doi:

Insert

3xFLAG-NLS/SV40-AcGFP-NES/PKI

Expression

Mammalian

Promoter

CMV

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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TAK8

Plasmid

#227096

Purpose

Luciferase reporter vector with wild type 5' Element (MMTV) insert

Depositor

Farah Mustafa

Publication

Khader et al Commun Biol. 2024 Nov 16;7(1):1521. doi:

Insert

CMV Promoter and R to 400bp Gag

Use

Luciferase

Promoter

CMV

Available since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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JD180

Plasmid

#229486

Purpose

Adenoviral helper plasmid containing inducible E2A and L4-22k/33k genes

Depositor

Nerea Zabaleta

Publication

Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

Insert

E2A, L4-22k/33k, mCitrine

Use

AAV and Adenoviral

Expression

Mammalian

Promoter

Tre (E2A, L4-22k/33k), ETR (mCitrine)

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV GFP

Plasmid

#213685

Purpose

Expresses Green Fluorescent Protein (GFP) with an N-terminal twin-Strep tag

Depositor

Thomas Kukar

Publication

Root et al Cell Rep. 2024 Nov 19;43(12):114985. doi

Insert

Twin-Strep GFP

Use

AAV

Tags

Twin-Strep

Promoter

cytomegalovirus enhancer/chicken β-actin promoter

Available since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-3xGFP11-mem-3xControlgRNA

Plasmid

#224583

Purpose

Ubiquitous expression plasmid with CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage, three membrane split GFP(11) reporter.

Depositor

Erica Hutchins

Publication

Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

Insert

3xGFP11

Use

CRISPR

Tags

Membrane Localization Signal

Mutation

Codon optimized

Available since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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