We narrowed to 19,676 results for: Cre

1. CROP-seq-opti

   Plasmid

   #106280

   Purpose

   A version of the CROP-seq plasmid as presented in Datlinger et al. that contains the sgRNA-(F+E)-combined optimized backbone for CRISPRi from Chen et al.

   Depositor

   Jay Shendure

   Article

   Hill et al Nat Methods. 2018 Feb 19. pii: nmeth.460

   Insert

   EF1a-Puro-WPRE-hU6-gRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMXsIP GFP-VMP1

   Plasmid

   #117417

   Purpose

   Expresses VMP1 tagged with GFP in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Morita et al J Cell Biol. 2018 Aug 9. pii: jcb.201804

   Insert

   VMP1 (VMP1 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Oct. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBabe_Hyg_cOVA_T2A_mStrawberry

   Plasmid

   #161737

   Purpose

   Expression of cOVA and mStrawberry

   Depositor

   Alec Kimmelman

   Article

   Yamamoto et al Nature. 2020 May;581(7806):100-105. doi:

   Insert

   cytoplasmic ovalbumin
   

   Use

   Retroviral

   Tags

   mStrawberry

   Expression

   Mammalian

   Available Since

   Dec. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PGK-H2BeGFP

   Plasmid

   #21210

   Depositor

   Mark Mercola

   Article

   Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

   Insert

   Phosphoglycerate kinase promoter (PGK1 Human)
   

   Use

   Lentiviral

   Tags

   H2B-eGFP

   Available Since

   July 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMSCV-SMYD2

   Plasmid

   #85658

   Purpose

   retroviral expression of human SMYD2

   Depositor

   Julien Sage

   Article

   Reynoird et al Genes Dev. 2016 Apr 1;30(7):772-85. doi:

   Insert

   SMYD2 (SMYD2 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   LTR

   Available Since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLentiRNAGuide_001 - hU6-RfxCas13d-DR1-BsmBI-EFS-Puro-WPRE

   Plasmid

   #138150

   Purpose

   For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

   Insert

   hU6_Rfxcas13d-DR1_BsmBI
   

   Use

   Lentiviral

   Mutation

   DR1 A>T

   Available Since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEGFP VAMP8

   Plasmid

   #42311

   Depositor

   Thierry Galli

   Article

   Paumet et al J Immunol. 2000 Jun 1;164(11):5850-7.

   Insert

   VAMP 8 (VAMP8 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBabe_Zeo_cOVA_T2A_mStrawberry

   Plasmid

   #161736

   Purpose

   Expression of cOVA and mStrawberry

   Depositor

   Alec Kimmelman

   Article

   Yamamoto et al Nature. 2020 May;581(7806):100-105. doi:

   Insert

   cytoplasmic ovalbumin
   

   Use

   Retroviral

   Tags

   mStrawberry

   Expression

   Mammalian

   Available Since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. EF1a_SOX10_P2A_Hygro_Barcode

   Plasmid

   #120479

   Purpose

   Barcoded lentiviral vector to express SOX10 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   SOX10 (SOX10 Human)
   

   Use

   Lentiviral

   Promoter

   EF1a

   Available Since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pECE-M2-PP1Cbeta

    Plasmid

    #31677

    Depositor

    Anne Brunet

    Article

    Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

    Insert

    protein phosphatase 1, catalytic subunit, beta isozyme (PPP1CB Human)
    

    Tags

    M2

    Expression

    Mammalian

    Available Since

    April 11, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pLenti6/V5-p53_R273H

    Plasmid

    #22934

    Depositor

    Bernard Futscher

    Article

    Junk et al Neoplasia. 2008 May . 10(5):450-61.

    Insert

    tumor protein 53 (TP53 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    R273H Mutant

    Available Since

    Jan. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGMC00028 (aka pTS0057)

    Plasmid

    #200832

    Purpose

    AAVS1 guide RNA cloned into the lentiSAM puro backbone

    Depositor

    Raj Chari

    Article

    Yang et al Sci Adv. 2024 Feb 16;10(7):eadj2445. doi

    Insert

    AAVS1 guide RNA
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Lenti-guide-puro mSptlc2 - 2

    Plasmid

    #198494

    Purpose

    lentiviral stable expression of mSptlc2 gRNA 2

    Depositor

    Suzanne Pfeffer

    Article

    Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

    Insert

    mSptlc2 (Sptlc2 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pIGc-Xph20

    Plasmid

    #133024

    Purpose

    Expresses Xph20 in bacteria

    Depositor

    Matthieu Sainlos

    Article

    Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

    Insert

    Xph20
    

    Tags

    His10

    Expression

    Bacterial

    Mutation

    S63K

    Promoter

    T7

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRG01-U6-DR-crRNA-BsmbI(x2)-6T; EFS-Puro-2A-Fluc-WPRE

    Plasmid

    #123362

    Purpose

    Lentiviral vector with empty U6 cassette containing LbCpf1 direct repeat and U6 terminator, with constitutive expression of puromycin resistance and Firefly luciferase.

    Depositor

    Sidi Chen

    Article

    Chow et al Nat Methods. 2019 May;16(5):405-408. doi

    Insert

    Firefly luciferase
    

    Use

    CRISPR, Lentiviral, and Luciferase

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLenti6/V5-p53_R175H

    Plasmid

    #22936

    Depositor

    Bernard Futscher

    Article

    Junk et al Neoplasia. 2008 May . 10(5):450-61.

    Insert

    tumor protein 53 (TP53 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    R175H Mutant

    Available Since

    March 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. HotPETase_pET21b

    Plasmid

    #215407

    Purpose

    Expression construct for HotPETase gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    HotPETase
    

    Expression

    Bacterial

    Mutation

    S121E/D186H/R280A/P181V/S207R/S214Y/Q119K/S213E/N…

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pZR158_Lenti-U6-gRNA-10xCS1-PP7_hPGK-PCP-P65-HSF1-Puro

    Plasmid

    #180273

    Purpose

    Lentiviral expression vector for CRISPRa-SAM sgRNA with 10x capture sequence, and PCP-P65-HSF1 cassette

    Depositor

    Alexander Marson

    Article

    Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLentiRNACRISPR_007 - TetO-NLS-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

    Plasmid

    #138149

    Purpose

    Expresses RfxCas13d in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    CasRx
    

    Use

    Lentiviral

    Tags

    NLS-HA

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLentiRNAGuide_002 - hU6-RfxCas13d-DR-BsmBI-EFS-Puro-WPRE

    Plasmid

    #138151

    Purpose

    For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    hU6_Rfxcas13d-DR_BsmBI
    

    Use

    Lentiviral

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge