We narrowed to 14,602 results for: pET

1. K560_AviC_coBirA

   Plasmid

   #129765

   Purpose

   Expresses Drosophila Kinesin-1 truncated at 560 and biotinylated at tail for single-molecule assays

   Depositor

   William Hancock

   Insert

   KHC (Khc Fly)
   

   Use

   Tags

   Avi-tag GLNDIFEAQKIEWH and His6

   Expression

   Bacterial

   Mutation

   Truncation at 559

   Promoter

   Available since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUG-124

   Plasmid

   #104635

   Purpose

   Level 2 construct

   Depositor

   Marco Trujillo

   Article

   Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

   Insert

   HIS-Ubq_UBA1_HA-UBC8_GST-AvrPtoB_EL4
   

   Use

   Tags

   HIS-Ubq, HA-E2, GST-E3

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDJSVT91

   Plasmid

   #109305

   Purpose

   Express periplasmic, outer membrane, or secreted proteins in E. coli - SufI signal (TAT) and His6 tag

   Depositor

   Daniel Slade

   Article

   Casasanta et al Methods Mol Biol. 2019;1960:123-138. doi

   Type

   Empty backbone

   Use

   Tags

   SufI 1-30, 6His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBD-0016

   Plasmid

   #12345

   Depositor

   Axel Brunger

   Article

   DeLaBarre et al Mol Cell. 2006 May 19. 22(4):451-62.

   Insert

   VCP R313K (Vcp Mouse)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   R313K, I206V*(see below)

   Promoter

   Available since

   Aug. 24, 2006

   Availability

   Academic Institutions and Nonprofits only

5. pCri-13a

   Plasmid

   #61321

   Purpose

   Protein expression.

   Depositor

   F Xavier Gomis-Ruth

   Article

   Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

   Insert

   Green fluorescent protein
   

   Use

   Tags

   His-tag and MISTIC-tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. K560_AviN_coBirA

   Plasmid

   #129766

   Purpose

   Expresses Drosophila Kinesin-1 truncated at 560 and biotinylated on the head for single-molecule assays

   Depositor

   William Hancock

   Insert

   KHC (Khc Fly)
   

   Use

   Tags

   Avi-tag GLNDIFEAQKIEWH and His6

   Expression

   Bacterial

   Mutation

   Truncation at 559

   Promoter

   Available since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDJSVT89

   Plasmid

   #109303

   Purpose

   Express periplasmic, outer membrane, or secreted proteins in E. coli - TamA signal and His6 tag

   Depositor

   Daniel Slade

   Article

   Casasanta et al Methods Mol Biol. 2019;1960:123-138. doi

   Type

   Empty backbone

   Use

   Tags

   TamA 1-27, 6His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pKEW215_AcrIIA13

   Plasmid

   #138281

   Purpose

   Expression of AcrIIA13 protein

   Depositor

   Jennifer Doudna

   Article

   Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

   Insert

   AcrIIA13
   

   Use

   CRISPR

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. 6xHis-MBP-huLb5Cas12a

   Plasmid

   #174684

   Purpose

   Expresses 6xHis-MBP tagged Lb5Cas12a

   Depositor

   Piyush Jain

   Article

   Nguyen et al medRxiv 2021.07.21

   Insert

   huLb5Cas12a
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis, MBP, TEV and NLS, 6xHis, 3xHA

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p2CT-Cdi

    Plasmid

    #83044

    Purpose

    Expresses Corynebacterium diphtheriae Cas9 (CdiCas9)

    Depositor

    Jennifer Doudna

    Article

    Ma et al Mol Cell. 2015 Nov 5;60(3):398-407. doi:

    Insert

    CdiCas9
    

    Use

    Tags

    His-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only