We narrowed to 11,847 results for: NSI
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Plasmid#112718PurposeExpresses YFP-KRAS4B fusion protein used for FRET studies in eukaryotic cells.DepositorInsertYFP-KRAS4B (KRAS Human)
ExpressionMammalianAvailable SinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 SARS-CoV-2 NSP5
Plasmid#141259PurposeGateway-compatible Entry vector, with insert of NSP5 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1DepositorHas ServiceCloning Grade DNAInsertNSP5 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vectorMutationMany synonymous changes due to codon optimizationAvailable SinceApril 1, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTT3-ecdICAM1-ST3-His
Plasmid#210666PurposeExpression of the extracellular domain of human ICAM1 fused to Spytag003 + Histag in HEK293 (or similar)DepositorInsertExtracellular domain of human ICAM-1 fused to Spytag003 and Histag (ICAM1 Human, Synthetic)
TagsHistag and Spytag003ExpressionMammalianPromoterCMVAvailable SinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-RAP1A-E63-puro
Plasmid#156167PurposeLentiviral vector for expression of RAP1A-E63, with puromycin selectionDepositorInsertRAP1A (RAP1A Human)
UseLentiviralTagsnoneExpressionMammalianMutationchanged Glutamine 63 to Glutamic acidPromoterhuman PGKAvailable SinceJuly 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMXs-puro-hMGT
Plasmid#172393PurposeRetroviral expression vector for human direct cardiac reprogrammingDepositorAvailable SinceJuly 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
LgBiT-hACE2
Plasmid#173431Purposeprotein expression plasmid of LgBiT-hACE2-IgG1 FcDepositorAvailable SinceJan. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTol2-UAS-zArchon1-KGC-EGFP-ER2
Plasmid#108427PurposeCodon-optimized Archon1 fluorescent voltage reporter in zebrafish expression plasmidDepositorInsertzArchon1-KGC-EGFP-ER2
UseZebrafishPromoterUASAvailable SinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-GATA6
Plasmid#101418PurposeDonor Vector containing GATA6 transcription factor, part of the Human TFome CollectionDepositorInsertGATA6 (GATA6 Human)
UseGateway shuttling vectorMutationLast nucleotide of stop codon removed to allow fo…Available SinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
mEos3.2GG
Plasmid#203756PurposeEncodes a modified version of the membrane anchor of KRas, including the last 16 residues of the C terminus, with mEos3.2 fused to the N terminus. The base pairs encoding the CAAX box for KRas were replaced by the CAAX box from rap1B, resulting in a geranylgeranylation instead of farnesylation. Used as a fluorescent plasma membrane probe.DepositorAvailable SinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmEos2-N1 GBP (GBP-mEos2)
Plasmid#162876PurposeExpression in mammalian cells of GFP binding protein (GBP) tagged with photoconvertable protein mEos2 to track GFP proteins of interest to perform Fluorescent intrabody Localization MicroscopyDepositorInsertGFP Binding Protein tagged with mEos2
TagsmEos2ExpressionMammalianAvailable SinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLX_TRC311 ETV5
Plasmid#74984PurposeConstitutive ETV5 ORF (codon optimized), blasticidin selectionDepositorExpressionMammalianMutationCodon optimized (synonymous)Available SinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTT3-ecdCD19-ST3-His_withSUMO
Plasmid#223219PurposeExpression of the extracellular domain of human CD19 fused to Spytag003 + Histag in HEK293(or similar) with SUMO for better protein expressionDepositorInsertextracellular domain of B-lymphocyte antigen CD19 (CD19 Human)
TagsHistag, SUMO, and SpytagExpressionMammalianPromoterCMVAvailable SinceMarch 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-iSeroSnFR-Nlgn
Plasmid#128485PurposeFluorescent reporter for serotonin (dendrite localization tag)DepositorHas ServiceAAV9InsertiSeroSnFR
UseAAVTagsFull-length neuroligin, Ig-kappa leader, and cpsf…ExpressionMammalianPromoterCAGAvailable SinceDec. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
EGFP-p300 (D1399Y)
Plasmid#191763PurposeExpression of EGFP fused to catalytically dead core p300 histone acetyltransferase (D1399Y)DepositorInsertFRB-EGFP-p300 core (D1399Y) (EP300 Human)
TagsEGFP (N-terminal of p300 core (D1399Y)) and FRB (…ExpressionBacterial and MammalianMutationcatalytic D1399Y mutation in p300PromoterEF1alphaAvailable SinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC19_mRuby-3xFLAG-NLS-3xSV40-poly(A)_loxP-PGK-Puro-loxP
Plasmid#195505PurposeT-REX17 donor plasmid to generate locus specific integration of mRuby followed by 3xSV40 poly(A) into Exon 1 of human lncRNA T-REX17DepositorInsertpT-REX-mRuby-3xFLAG-NLS-3xSV40-poly(A)_loxP-PGK-Puro-loxP_T-REX(Ex1)
UseCRISPR; Donor vectorTagsmRuby-3xFLAG-NLS-3xSV40-poly(A)ExpressionMammalianAvailable SinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCVpuro-Mito-DsRed1
Plasmid#87379PurposeRetroviral transfection of cells to express DsRed1 (Ex555, Em585) localising to the mitochondrial matrix via the transit peptide from human TXN2 (Thioredoxin).DepositorInsertTXN2 mitochondrial transit peptide (TXN2 Human)
UseRetroviralTagsDsRed1ExpressionMammalianMutationFirst 60 aa onlyPromoterRetroviral 5' LTR promoterAvailable SinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lenti-CMV-PE2-IRES-ZeoR
Plasmid#207352PurposeLentiviral transfer plasmid containing CMV-driven expression cassette encoding PE2 enzyme for prime editing.DepositorInsertPrime editor 2 (PE2) enzyme
UseLentiviralAvailable SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX304_zeo_mmFbxw7a
Plasmid#160102PurposeExpresses murine Fbxw7 alpha in mammalian cellsDepositorAvailable SinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti_EF1a_Flag_TP53_R248W+P278A
Plasmid#183115PurposeExpresses flag-tagged p53 with both R248W and P278A mutations in mammalian cellsDepositorInsertTP53 (TP53 Human)
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationchanged proline 278 to alanine and arginine 248 t…PromoterEF1aAvailable SinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCW-ND-Cdt1Δ499-546-HA-Puro
Plasmid#193768PurposeDoxycyline-inducible (TetOn) human Cdt1 (SDV40 NLS , C-terminal HA ) w/ mutations stopping S phase degradation and in MCM binding domain (Δ499-546), expressed from all-in-one pCW vector (TetOn + rtTA)DepositorInsertND-Cdt1Δ499-546 (CDT1 Human)
UseLentiviralTagsHAMutationamino acids 1-19 deleted, ΔCy mutation (aa68-70 t…PromoterTREAvailable SinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only