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Plasmid#158035Purposelenti-viral construct with Cre recombinase and U6 driven sgRNA against mouse Ripk4DepositorInsertRipk4 sgRNA
UseTagsExpressionMammalianMutationPromoterU6Available sinceSept. 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pPICZ-His6-K2P4.1A-mCherry-StreptagII
Plasmid#158743PurposeExpresses K2P4.1 isoform A with TEV protease cleavable N- and C-terminal affinity tags.DepositorInsertK2P4.1-A (KCNK4 Human)
UseTagsHis6 and mCherry with Streptag IIExpressionYeastMutationResidues 1-264 with N-linked glycosylation sites …PromoterAOX1Available sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-His6-K2P4.1B-mCherry-StreptagII
Plasmid#158744PurposeExpresses K2P4.1 isoform B with TEV protease cleavable N- and C-terminal affinity tags.DepositorInsertK2P4.1-B (KCNK4 Human)
UseTagsHis6 and mCherry with Streptag IIExpressionYeastMutationResidues 1-290 and N-linked glycosylation sites r…PromoterAOX1Available sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHJ4
Plasmid#158785PurposePan-neuronal nuclear-localized GCaMP expression in C. elegansDepositorInsertPrab-3::nls::GCaMP6m
UseTagsNLSExpressionWormMutationPromoterAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-HiGlpG- S116A, H196A
Plasmid#158539PurposeBacterial expression of rhomboid protease catalytic mutant HiGlpG-S116A,H196ADepositorInsertHiGlpG
UseTagsGST tagExpressionBacterialMutationS116A;H169APromoterAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-S*HiGlpG
Plasmid#158534PurposeBacterial expression of rhomboid protease mutant S*HiGlpGDepositorInsertS*HiGlpG
UseTagsGST tagExpressionBacterialMutationSector 1 and 2 mutationsPromoterAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-S1*HiGlpG
Plasmid#158535PurposeBacterial expression of rhomboid protease mutant S1*HiGlpGDepositorInsertS1*HiGlpG
UseTagsGST tagExpressionBacterialMutationSector 1 mutationsPromoterAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-S2*HiGlpG
Plasmid#158536PurposeBacterial expression of rhomboid protease mutant S2*HiGlpGDepositorInsertS2*HiGlpG
UseTagsGST tagExpressionBacterialMutationSector 2 mutationsPromoterAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-delN-EcGlpG
Plasmid#158537PurposeBacterial expression of rhomboid protease EcGlpG without N terminusDepositorInsertdelta N-terminus EcGlpG
UseTagsGST tagExpressionBacterialMutationN-terminal deletion (1-86)PromoterAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA3
Plasmid#158481PurposeEntry clone for Golden Gate assembly. Golden Gate compatible A. thaliana ZAR1 L17E with STOP codonDepositorInsertZAR1 (ZAR1 Mustard Weed)
UseGolden gate entry vectorTagsExpressionMutationL17E, STOP codonPromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA44
Plasmid#158522PurposeIn planta gene expression of Golden gate compatible A. thaliana ZAR1 D498V/L17E-eGFP. Km plant selection marker.DepositorInsertZAR1-eGFP (ZAR1 Mustard Weed)
UseTagseGFPExpressionPlantMutationL17E/D489VPromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA22
Plasmid#158500PurposeIn planta gene expression of Golden gate compatible N. benthamiana ZAR1 D481V. Km plant selection marker.DepositorInsertZAR1
UseTagsExpressionPlantMutationD481VPromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3xFLAG-MAVS-Gln93,Ala271
Plasmid#158632PurposeGateway entry vector for an inducible 3xFLAG-tagged MAVS mutantDepositorInsertMAVS (MAVS Human)
UseGateway entry vectorTags3XFLAGExpressionMutationE93Q/Q271APromoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3xFLAG-MAVS-Ala148,Ala271
Plasmid#158633PurposeGateway entry vector for an inducible 3xFLAG-tagged MAVS mutantDepositorInsertMAVS (MAVS Human)
UseGateway entry vectorTags3XFLAGExpressionMutationQ148A/Q271APromoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLIK 3xFLAG-MAVS-Ala148,Ala271 hygro
Plasmid#158639PurposeLentiviral expression vector for an inducible 3xFLAG-tagged MAVS mutant constructDepositorInsertMAVS (MAVS Human)
UseLentiviralTags3XFLAGExpressionMutationQ148A/Q271APromoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3xFLAG-MAVS-Ala148
Plasmid#158630PurposeGateway entry vector for an inducible 3xFLAG-tagged MAVS mutantDepositorInsertMAVS (MAVS Human)
UseGateway entry vectorTags3XFLAGExpressionMutationQ148APromoterAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLIK 3xFLAG-MAVS-Ala148 hygro
Plasmid#158636PurposeLentiviral expression vector for an inducible 3xFLAG-tagged MAVS mutant constructDepositorInsertMAVS (MAVS Human)
UseLentiviralTags3XFLAGExpressionMutationQ148APromoterAvailable sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLIK 3xFLAG-MAVS-Gln93 hygro
Plasmid#158635PurposeLentiviral expression vector for an inducible 3xFLAG-tagged MAVS mutant constructDepositorInsertMAVS (MAVS Human)
UseLentiviralTags3XFLAGExpressionMutationE93QPromoterAvailable sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA16
Plasmid#158494PurposeEntry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 D481V/L17E without STOP codonDepositorInsertZAR1
UseGolden gate entry vectorTagsExpressionMutationL17E, D481V, no STOP codonPromoterAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-PHKG1-V5-miniTurbo_IDG-K
Plasmid#158192PurposeGateway destination clone of PHKG1 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertPHKG1-V5-miniTurbo (PHKG1 Human)
UseLentiviral; Gateway destinationTagsV5-miniTurboExpressionMammalianMutationPromoterUbiquitinAvailable sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only