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pET28a-IMX-DogTag

Plasmid

#113765

Purpose

Expresses IMX-DogTag in bacterial cells for modular antigen multimerization using SnoopLigase

Depositor

Mark Howarth

Article

Andersson et al Sci Rep. 2019 Mar 15;9(1):4625. doi: 10.

Insert

IMX-DogTag

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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S48I48 ELP

Plasmid

#68394

Purpose

Elastin-like polypeptide (VPGxG) with 48 repeats of serine guest residue followed by 48 repeats of isoleucine guest residue

Depositor

Andrew MacKay

Article

Shah et al Protein Sci. 2012 Jun;21(6):743-50. doi:

Insert

S48I48 ELP

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pET28a-SUMO-DogTag

Plasmid

#105629

Purpose

Plasmid for recombinant expression in bacteria of SUMO domain fused to DogTag, permitting covalent linkage to SnoopTagJr

Depositor

Mark Howarth

Article

Buldun et al J Am Chem Soc. 2018 Feb 28;140(8):3008-3

Insert

SUMO-DogTag

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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PGRP-SA

Plasmid

#21055

Depositor

Johann Deisenhofer

Article

Chang et al PLoS Biol. 2004 Sep . 2(9):E277.

Insert

PGRP-SA (PGRP-SA Fly)

Use

Tags

His

Expression

Insect

Mutation

Promoter

Available since

Sept. 8, 2009

Availability

Academic Institutions and Nonprofits only
pET28a-SnoopTagJr-MBP

Plasmid

#105628

Purpose

Plasmid for recombinant expression in bacteria of MBP fused to SnoopTagJr, permitting covalent linkage to DogTag by SnoopLigase

Depositor

Mark Howarth

Article

Buldun et al J Am Chem Soc. 2018 Feb 28;140(8):3008-3

Insert

SnoopTagJr-MBP

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pRset-OppA

Plasmid

#207869

Purpose

Bacterial expression of poly-his-tagged L. lactic OppA

Depositor

Mathew Tantama

Article

Norcross et al ACS Omega. 2019 Feb 28;4(2):2812-2822. d

Insert

OppA

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pH6-spTorA-mCherry

Plasmid

#169040

Purpose

mCherry preceeded by the signal peptide of TorA (spTorA) with an N-terminal 6xHis tag in pET28a.

Depositor

Siegfried Musser

Article

Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi

Insert

H6-spTorA-mCherry

Use

Tags

6xHis and spTorA (signal peptide of E. coli TorA)

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pQE30-AtAEP2

Plasmid

#159529

Purpose

Expresses Arabidopsis thaliana asparaginyl endopeptidase 2 in E. coli

Depositor

Joshua Mylne

Article

Bernath-Levin et al Chem Biol. 2015 May 21;22(5):571-82. doi

Insert

Arabidopsis thaliana asparaginyl endopeptidase 2

Use

Tags

Six-Histidine tag

Expression

Bacterial

Mutation

Promoter

T5

Available since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pQE30-CeAEP1

Plasmid

#159528

Purpose

Expresses jack bean (Canavalia ensiformis) asparaginyl endopeptidase 1 in E. coli

Depositor

Joshua Mylne

Article

Bernath-Levin et al Chem Biol. 2015 May 21;22(5):571-82. doi

Insert

Canavalia ensiformis asparaginyl endopeptidase 1

Use

Tags

Six-Histidine tag

Expression

Bacterial

Mutation

Promoter

T5

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pQE30-HaAEP1

Plasmid

#159530

Purpose

Expresses sunflower (Helianthus annuus) asparaginyl endopeptidase 1 in E. coli

Depositor

Joshua Mylne

Article

Bernath-Levin et al Chem Biol. 2015 May 21;22(5):571-82. doi

Insert

Helianthus annuus asparaginyl endopeptidase 1 (LOC110922574 Synthetic)

Use

Tags

Six-Histidine tag

Expression

Bacterial

Mutation

Promoter

T5

Available since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pLp_3050sNuc

Plasmid

#122030

Purpose

Plasmid for heterologous protein secretion in Lactobacillus plantarum and L. sakei

Depositor

Vincent Eijsink, Geir Mathiesen

Article

Mathiesen et al BMC Genomics. 2009 Sep 10;10:425. doi: 1

Insert

signal peptide Lp_3050

Use

Tags

Expression

Bacterial

Mutation

Promoter

sppA

Available since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pSSN6

Plasmid

#104340

Purpose

Express SSN6

Depositor

Alexander Varshavsky

Article

Xia et al J Biol Chem. 2008 Oct 24;283(43):28958-6

Insert

SSN6 (CYC8 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pTUP1

Plasmid

#104341

Purpose

Express TUP1

Depositor

Alexander Varshavsky

Article

Xia et al J Biol Chem. 2008 Oct 24;283(43):28958-6

Insert

TUP1 (TUP1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Dec. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pJFRC7-UAS-NPRR_ANP

Plasmid

#133243

Purpose

Fluorescent reporter for neuropeptide release imaging

Depositor

David Anderson

Article

Ding et al Elife. 2019 Jun 26;8. pii: 46421. doi: 1

Insert

rat atrial natriuretic peptide-fused codon-optimized GCaMP6s

Use

Tags

Expression

Insect

Mutation

Promoter

hsp70

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pET16b-Antp

Plasmid

#86866

Purpose

Bacterial expression of fusion protein with cell penetrating peptide, antennapedia fragment, at C-terminus.

Depositor

Michael Chin

Article

Chien et al Genesis. 2014 Jul;52(7):695-701. doi: 10

Insert

Linker-Antp

Use

Tags

Antp

Expression

Bacterial

Mutation

Promoter

Available since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-(RTRG)6

Plasmid

#69849

Purpose

The RTRG repeat act as a nuclear/cytoplasmic partition signal in the expressed fusion protein providing increased nuclear to cytoplasmic partition ratio when increasing the number of repeats

Depositor

Daniel Christophe

Article

Christophe-Hobertus et al Protein Pept Lett. 2008;15(4):397-401.

Insert

6 RTRG repeats

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pET41c-PhTET2

Plasmid

#182428

Purpose

Expresses P. horikoshii tetrahedral aminopeptidase 2 (TET2) in E. coli

Depositor

Bruno Franzetti

Article

Gauto et al Nat Commun. 2022 Apr 8;13(1):1927. doi:

Insert

Tetrahedral aminopeptidase TET2 from P. hirokoshii

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-RTRG

Plasmid

#69845

Purpose

The RTRG repeat act as a nuclear/cytoplasmic partition signal in the expressed fusion protein providing increased nuclear to cytoplasmic partition ratio when increasing the number of repeats

Depositor

Daniel Christophe

Article

Christophe-Hobertus et al Protein Pept Lett. 2008;15(4):397-401.

Insert

1 RTRG repeat

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 13, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-(RTRG)2

Plasmid

#69846

Purpose

The RTRG repeat act as a nuclear/cytoplasmic partition signal in the expressed fusion protein providing increased nuclear to cytoplasmic partition ratio when increasing the number of repeats

Depositor

Daniel Christophe

Article

Christophe-Hobertus et al Protein Pept Lett. 2008;15(4):397-401.

Insert

2 RTRG repeats

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 13, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-(RTRG)3

Plasmid

#69847

Purpose

The RTRG repeat act as a nuclear/cytoplasmic partition signal in the expressed fusion protein providing increased nuclear to cytoplasmic partition ratio when increasing the number of repeats

Depositor

Daniel Christophe

Article

Christophe-Hobertus et al Protein Pept Lett. 2008;15(4):397-401.

Insert

3 RTRG repeats

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 13, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge