We narrowed to 22,181 results for: TES

1. TRS6E1b-luc(-732/-721) mutant 3

   Plasmid

   #45386

   Depositor

   Thomas Gelehrter

   Article

   Song et al J Biol Chem. 1998 Nov 6;273(45):29287-90

   Insert

   6 copies of hPAI-1 promoter -732/-721 mutated at -723/-721
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   -723/-721 mutated from tgt to aca

   Available Since

   June 20, 2013

   Availability

   Academic Institutions and Nonprofits only

2. TRS6E1b-luc(-732/-721) mutant 2

   Plasmid

   #45385

   Depositor

   Thomas Gelehrter

   Article

   Song et al J Biol Chem. 1998 Nov 6;273(45):29287-90

   Insert

   6 copies of hPAI-1 promoter -732/-721 mutated at -730/-728
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   mutated at -730/-728 from aca to tgt

   Available Since

   June 20, 2013

   Availability

   Academic Institutions and Nonprofits only

3. TRS6E1b-luc(-732/-721) mutant1

   Plasmid

   #45384

   Depositor

   Thomas Gelehrter

   Article

   Song et al J Biol Chem. 1998 Nov 6;273(45):29287-90

   Insert

   6 copies of hPAI1 promoter -732/-721 mutated at -732/-731
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   mutated -732/-731 from ag to tc

   Available Since

   June 20, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro

   Plasmid

   #71236

   Purpose

   Express sgRNA and dCas9-KRAB from lentiviral vector

   Depositor

   Charles Gersbach

   Article

   Thakore et al Nat Methods. 2015 Dec;12(12):1143-9. doi

   Inserts

   humanized dCas9-KRAB T2A Puro
   sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag

   Mutation

   D10A and H840A

   Available Since

   Dec. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSPgRNA

   Plasmid

   #47108

   Purpose

   Expresses a S. pyogenes Cas9/dCas9 guide RNA in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

   Has Service

   Cloning Grade DNA

   Insert

   SPgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. CDK1as_T2A_puromycin

   Plasmid

   #118595

   Purpose

   One of three plasmids required to create CDK1as human cells by single transfection (One shot system). CDK1as cDNA and puromycin resistance cassette flanked by Sleeping Beauty transposon.

   Depositor

   William Earnshaw

   Article

   Saldivar et al Science. 2018 Aug 24;361(6404):806-810.

   Insert

   CDK1
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. p5E-mpeg1.1

   Plasmid

   #75023

   Purpose

   Can be used to drive expression in zebrafish microglia.

   Depositor

   Nicholas Cole

   Article

   Don et al Zebrafish. 2016 Sep 15.

   Insert

   macrophage expressed 1, tandem duplicate 1 (mpeg1.1 Zebrafish)
   

   Use

   5' entry vector for multisite gateway three …

   Promoter

   mpeg1.1

   Available Since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP

   Plasmid

   #71237

   Purpose

   Express sgRNA and dCas9-KRAB from 3rd generation lentiviral vector

   Depositor

   Charles Gersbach

   Article

   Thakore et al Nat Methods. 2015 Dec;12(12):1143-9. doi

   Inserts

   humanized dCas9-KRAB T2A GFP
   sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag

   Mutation

   D10A and H840A

   Available Since

   Dec. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDRM98 mKate2 Blast

   Plasmid

   #174720

   Purpose

   Expresses the NLS-tagged mKate2 gene, P2A and the blasticidin resistance gene.

   Depositor

   Wayne Tilley

   Article

   Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413

   Inserts

   mKate2
   BSD
   

   Use

   Lentiviral

   Tags

   P2A linker and SV40 NLS with GGS linker

   Expression

   Mammalian

   Promoter

   MND and MND (off mKate-P2A)

   Available Since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. mVenusN-Cavin1

    Plasmid

    #108887

    Purpose

    Expression of Cavin1 fused to the N-terminus of mVenus for bimolecular fluorescence complementation

    Depositor

    Rob Parton

    Article

    Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

    Insert

    caveolae associated protein 1 (CAVIN1 Human, Synthetic)
    

    Tags

    mVenus1-155

    Expression

    Mammalian

    Mutation

    altered codon usage

    Available Since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. mVenusC-Cavin1

    Plasmid

    #108888

    Purpose

    Expression of Cavin1 fused to the C-terminus of mVenus for bimolecular fluorescence complementation

    Depositor

    Rob Parton

    Article

    Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

    Insert

    Cavin1 (altered codon usage) (CAVIN1 Human, Synthetic)
    

    Tags

    mVenus156-239

    Expression

    Mammalian

    Mutation

    altered codon usage

    Available Since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA-dCas9

    Plasmid

    #47106

    Purpose

    Expresses inactivated S. pyogenes dCas9 (D10A, H840A) in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Flag and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive)

    Promoter

    CMV

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-EB3mCherry_cmv-LifeActGFP

    Plasmid

    #133927

    Purpose

    Live dual labeling of the actin network and polymerizing microtubules for dynamic analysis of the cytoskeleton

    Depositor

    Frank Bradke, Sebastián Dupraz

    Article

    Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

    Insert

    EB3-mCherry and Lifeact-GFP (MAPRE3 Human)
    

    Expression

    Mammalian

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. GFP-Trak1

    Plasmid

    #127621

    Purpose

    N-terminal GFP-tagged mouse Trak1

    Depositor

    Josef Kittler

    Article

    Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

    Insert

    Trak1 (Trak1 Mouse)
    

    Tags

    eGFP

    Expression

    Mammalian

    Available Since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV-U6-gRNA-UbC-DsRed-P2A-Bsr

    Plasmid

    #83919

    Purpose

    Lentiviral SpCas9-gRNA expression vector with DsRed-Express2-P2A-BlastR

    Depositor

    Charles Gersbach

    Article

    Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

    Inserts

    DsRed-Express2
    Bsr
    

    Use

    CRISPR and Lentiviral

    Tags

    P2A

    Promoter

    hUbC

    Available Since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pWMD23

    Plasmid

    #140006

    Purpose

    Expression of ORF1 (Shuffle 1 NLS) and Pong TPase (L418A, L420A)

    Depositor

    Charles Nathan Hancock

    Article

    Johnson et al Plant Direct. 2021 Jan 19;5(1):e00300. d

    Inserts

    ORF1 Shuffle 1 NLS
    Pong TPase LA
    

    Expression

    Plant

    Mutation

    NES sequence mutated L418A, L420A and Ping N-term…

    Available Since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. actc1b-mKate2-Dnm2a

    Plasmid

    #109519

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to Dynamin2a

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    Dnm2a (dnm2a Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    mKate2

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. CMV-R-GECO1.2

    Plasmid

    #45494

    Purpose

    Red intensiometric genetically encoded Ca2+-indicators for optical imaging 1.2

    Depositor

    Robert Campbell

    Article

    Wu et al ACS Chem Neurosci. 2013 Mar 19.

    Insert

    R-GECO1.2
    

    Expression

    Mammalian

    Mutation

    R-GECO1 M164R/I166V/V174L/F222L/N267S/S270T/I330M…

    Promoter

    CMV

    Available Since

    July 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. mKate2-P2A-APEX2-GBP

    Plasmid

    #67650

    Purpose

    Mammalian expression vector driving APEX2-GBP (EM peroxidase marker fused to GFP-targeting nanobody). Also contains bicistronially expressed cytoplasmic red fluorescent reporter.

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    APEX2-GBP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GB-NES

    Plasmid

    #61017

    Purpose

    ddGFP B-NES

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    ddGFP B
    

    Tags

    HindIII site and NES sequence LALKLAGLDIGS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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