We narrowed to 159,238 results for: addgene

1. pPyCAG-Nanog(Q124A)-IP

   Plasmid

   #62993

   Purpose

   Expresses Mouse Nanog Q124A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).

   Depositor

   Shinya Yamanaka

   Insert

   Mouse Nanog Q124A Mutant (Nanog Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Changed Glutamine 124 (125 AA in Mouse Protein) t…

   Promoter

   Available since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pPyCAG-Nanog(M125A)-IP

   Plasmid

   #62994

   Purpose

   Expresses Mouse Nanog M125A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).

   Depositor

   Shinya Yamanaka

   Insert

   Mouse Nanog M125A Mutant (Nanog Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Changed Methionine 125 (126 AA in Mouse Protein) …

   Promoter

   Available since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pPyCAG-Nanog(Q138A)-IP

   Plasmid

   #62997

   Purpose

   Expresses Mouse Nanog Q138A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).

   Depositor

   Shinya Yamanaka

   Insert

   Mouse Nanog Q138A Mutant (Nanog Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Changed Glutamine 138 (139 AA in Mouse Protein) t…

   Promoter

   Available since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pPyCAG-Nanog(T141A)-IP

   Plasmid

   #62999

   Purpose

   Expresses Mouse Nanog T141A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).

   Depositor

   Shinya Yamanaka

   Insert

   Mouse Nanog T141A Mutant (Nanog Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Changed Threonine 141 (142 AA in Mouse Protein) t…

   Promoter

   Available since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pPyCAG-Nanog(Q144A)-IP

   Plasmid

   #63000

   Purpose

   Expresses Mouse Nanog Q144A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).

   Depositor

   Shinya Yamanaka

   Insert

   Mouse Nanog Q144A Mutant (Nanog Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Changed Glutamine 144 (145 AA in Mouse Protein) t…

   Promoter

   Available since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pPyCAG-Nanog(N145A)-IP

   Plasmid

   #63001

   Purpose

   Expresses Mouse Nanog N145A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).

   Depositor

   Shinya Yamanaka

   Insert

   Mouse Nanog N145A Mutant (Nanog Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Changed Asparagine 145 (146 AA in Mouse Protein) …

   Promoter

   Available since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pPyCAG-Nanog(R147A)-IP

   Plasmid

   #63002

   Purpose

   Expresses Mouse Nanog R147A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).

   Depositor

   Shinya Yamanaka

   Insert

   Mouse Nanog R147A Mutant (Nanog Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Changed Arginine 147 (148 AA in Mouse Protein) to…

   Promoter

   Available since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pPyCAG-Nanog(K151A)-IP

   Plasmid

   #63004

   Purpose

   Expresses Mouse Nanog K151A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).

   Depositor

   Shinya Yamanaka

   Insert

   Mouse Nanog K151A Mutant (Nanog Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Changed Lysine 151 (152 AA in Mouse Protein) to A…

   Promoter

   Available since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pPyCAG-Nanog(T100A)-IP

   Plasmid

   #62989

   Purpose

   Expresses Mouse Nanog T100A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).

   Depositor

   Shinya Yamanaka

   Insert

   Mouse Nanog T100A Mutant (Nanog Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Changed Threonine 100 (101AA) to Alanine

   Promoter

   CAG

   Available since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pPyCAG-Nanog(F102A)-IP

    Plasmid

    #62990

    Purpose

    Expresses Mouse Nanog F102A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).

    Depositor

    Shinya Yamanaka

    Insert

    Mouse Nanog F102A Mutant (Nanog Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Changed Phenylalanine 102 (103AA in Mouse Protein…

    Promoter

    Available since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTF257 (pFA6a-TEV-6xGly-His3MX)

    Plasmid

    #44068

    Depositor

    Tim Formosa

    Type

    Empty backbone

    Use

    Pcr-based yeast c-terminal tagging

    Tags

    (XhoI-KpnI-XbaI)-containing spacer, 6xGly linker,…

    Expression

    Mutation

    Promoter

    Available since

    April 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTF258 (pFA6a-PSc-6xGly-His3MX)

    Plasmid

    #44062

    Depositor

    Tim Formosa

    Type

    Empty backbone

    Use

    Pcr-based yeast c-terminal tagging

    Tags

    (XhoI-KpnI-XbaI)-containing spacer, 6xGly linker,…

    Expression

    Mutation

    Promoter

    Available since

    April 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTF261 (pFA6a-TEV-6xGly-HphMX)

    Plasmid

    #44080

    Depositor

    Tim Formosa

    Type

    Empty backbone

    Use

    Pcr-based yeast c-terminal tagging

    Tags

    (XhoI-KpnI-XbaI)-containing spacer, 6xGly linker,…

    Expression

    Mutation

    Promoter

    Available since

    April 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTF262 (pFA6a-PSc-6xGly-HphMX)

    Plasmid

    #44074

    Depositor

    Tim Formosa

    Type

    Empty backbone

    Use

    Pcr-based yeast c-terminal tagging

    Tags

    (XhoI-KpnI-XbaI)-containing spacer, 6xGly linker,…

    Expression

    Mutation

    Promoter

    Available since

    April 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTF259 (pFA6a-TEV-6xGly-KanMX)

    Plasmid

    #44092

    Depositor

    Tim Formosa

    Type

    Empty backbone

    Use

    Pcr-based yeast c-terminal tagging

    Tags

    (XhoI-KpnI-XbaI)-containing spacer, 6xGly linker,…

    Expression

    Mutation

    Promoter

    Available since

    April 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PP-MAPK2 (3RP9)

    Plasmid

    #32862

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    MAPK2
    

    Use

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Mutation

    amino acids 123-548 only, P421L

    Promoter

    Available since

    Jan. 10, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

17. MPP8 (3R93)

    Plasmid

    #32050

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    MPP8 (MPHOSPH8 Human)
    

    Use

    Tags

    His (with TEV cleavage)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 6, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

18. integrin beta6 Y762F pBabe puro

    Plasmid

    #13597

    Depositor

    Dean Sheppard

    Insert

    integrin beta 6 Y762F (ITGB6 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Y762F

    Promoter

    Available since

    Jan. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

19. pLIX403_Capture1_APOBEC_HA_P2A_mRuby

    Plasmid

    #183901

    Purpose

    Inducible lentiviral expression, TRE-gateway-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTA

    Depositor

    Eugene Yeo

    Article

    Brannan et al Nat Methods. 2021 May;18(5):507-519. doi

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector, doxycycli…

    Tags

    Capture1_APOBEC_HA_P2A_mRuby

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet ON

    Available since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-anti-PDL1(KN035) PAGER(TF)

    Plasmid

    #230003

    Purpose

    Expresses anti-PDL1 PAGER(TF) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)

    Depositor

    Alice Ting

    Article

    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

    Insert

    IL2SP-Aro6-KN035-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    V360A/R361A on KORD

    Promoter

    CMV

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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