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Plasmid#143229PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertRBPJ (RBPJ Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
OPRK1-DuET
Plasmid#213359PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertOPRK1 (OPRK1 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF3253
Plasmid#144729PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertRBPJ (RBPJ Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
JWW-1 human chimeric antibody
Plasmid#66748PurposeExpresses a rat/human chimeric IgG1 HPV16 L2-specific neutralizing antibody that recognizes HPV16 L2 amino acid region 17-36. JWW-1 works in ELISA/WB/HPV Neutralization assay.DepositorInsertsJWW-1 Heavy Chain Gamma
JWW-1 Light Chain Kappa
UseTagsExpressionMammalianMutationFirst 3 amino acids of rat light chain constant r…PromotermEF1 and rEF1Available sinceJuly 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
TFORF0612
Plasmid#143054PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertRBPJ (RBPJ Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
actin CP alpha 2 beta 2
Plasmid#13452DepositorUseTagsExpressionBacterialMutationnonePromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
TFORF0611
Plasmid#143230PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertRBPJ (RBPJ Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-PTPRK-COMP5AP-AviTag-9xHis
Plasmid#157522PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertPTPRK (PTPRK Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
CDC42BPG gRNA (BRDN0001162420)
Plasmid#77525Purpose3rd generation lentiviral gRNA plasmid targeting human CDC42BPGDepositorInsertCDC42BPG (CDC42BPG Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
CDC42BPG gRNA (BRDN0001147621)
Plasmid#77526Purpose3rd generation lentiviral gRNA plasmid targeting human CDC42BPGDepositorInsertCDC42BPG (CDC42BPG Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
CDC42BPG gRNA (BRDN0001162561)
Plasmid#77527Purpose3rd generation lentiviral gRNA plasmid targeting human CDC42BPGDepositorInsertCDC42BPG (CDC42BPG Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
CDC42BPG gRNA (BRDN0001144777)
Plasmid#77528Purpose3rd generation lentiviral gRNA plasmid targeting human CDC42BPGDepositorInsertCDC42BPG (CDC42BPG Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-PTPRK-Fc(DAPA)-AviTag-6xHis
Plasmid#156958PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertPTPRK (PTPRK Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-iSeroSnFR
Plasmid#128484PurposeFluorescent reporter for serotonin (mammalian expression, membrane localization tag)DepositorInsertiSeroSnFR
UseTagsIg-kappa leader, Myc, PDGFR + enhanced ER export,…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-iSeroSnFR-Nlgn
Plasmid#128485PurposeFluorescent reporter for serotonin (dendrite localization tag)DepositorHas ServiceAAV9InsertiSeroSnFR
UseAAVTagsFull-length neuroligin, Ig-kappa leader, and cpsf…ExpressionMammalianMutationPromoterCAGAvailable sinceDec. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSyn.FAPdL5-POST-T2A-dTomato.WPRE.bGH
Plasmid#105981PurposeThis AAV plasmid constitutively expresses an extracellular dL5 FAP fused to the neuroligin-1 cytoplasmic domain for targeting to the synapse with a cell fill dTomato.DepositorInserthSyn-Igkappa-myc-FAPdL5-POST-T2A-dTomato-WPRE-bGH
UseAAVTagsFAPdL5-POST, T2A-dTomato, and mycExpressionMutationPromoterhuman synapsin 1Available sinceOct. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FLEx-iSeroSnFR
Plasmid#128486PurposeFluorescent reporter for serotonin (conditional + membrane localization tag)DepositorHas ServiceAAV9InsertiSeroSnFR
UseAAVTagsIg-kappa leader, Myc, PDGFR, and cpsfGFPExpressionMammalianMutationPromoterCAGAvailable sinceDec. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_RBPJ_WT
Plasmid#82158PurposeGateway Donor vector containing RBPJ , part of the Target Accelerator Plasmid Collection.DepositorInsertRBPJ (RBPJ Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
Capping Protein
Plasmid#89950PurposeF-actin-capping proteins bind in a Ca2+-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends.DepositorInsertsUseTags6*HisExpressionBacterialMutationV222IPromoterAvailable sinceJuly 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FLEx-iSeroSnFR-EnhancedExport
Plasmid#129180PurposeFluorescent reporter for serotonin imaging (conditional + membrane localization tag with enhanced export sequence)DepositorInsertiSeroSnFR
UseAAVTagsIg-kappa leader, Myc, and PDGFR + enhanced ER exp…ExpressionMutationPromoterCAGAvailable sinceDec. 23, 2020AvailabilityAcademic Institutions and Nonprofits only