We narrowed to 6,573 results for: tac

1. Dcp1 H40A-EGFP

   Plasmid

   #201421

   Purpose

   Bacterial protein expression of Dcp1 H40A

   Depositor

   Michael Rosen

   Article

   Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

   Insert

   Dcp1 (DCP1 Budding Yeast)
   

   Use

   Tags

   EGFP, TEV, 6xHis and MBP, TEV

   Expression

   Bacterial

   Mutation

   H40A

   Promoter

   tac

   Available since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. Pat1-mCh

   Plasmid

   #201415

   Purpose

   Bacterial protein expression of Pat1

   Depositor

   Michael Rosen

   Article

   Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

   Insert

   Pat1 (PAT1 Budding Yeast)
   

   Use

   Tags

   MBP, TEV and mCherry, TEV, 6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. FKBP-EGFP-PML peptide

   Plasmid

   #175247

   Purpose

   Bacterial expression and purification, low affinity SUMOylation substrate with a relatively high Km, can be recruited to FRB with rapamycin

   Depositor

   Michael Rosen

   Article

   Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

   Insert

   PML (PML Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Intersectin SH3 domain B

   Plasmid

   #47397

   Depositor

   Peter McPherson

   Article

   Yamabhai et al J Biol Chem. 1998 Nov 20;273(47):31401-7

   Insert

   Intersectin SH3 domain B
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   SH3 domain B

   Promoter

   tac

   Available since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

5. Intersectin SH3 domain C

   Plasmid

   #47398

   Depositor

   Peter McPherson

   Article

   Yamabhai et al J Biol Chem. 1998 Nov 20;273(47):31401-7

   Insert

   Intersectin SH3 domain C
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   SH3 domain C

   Promoter

   tac

   Available since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

6. Intersectin SH3 domain E

   Plasmid

   #47400

   Depositor

   Peter McPherson

   Article

   Yamabhai et al J Biol Chem. 1998 Nov 20;273(47):31401-7

   Insert

   Intersectin SH3 domain E
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   SH3 domain E

   Promoter

   tac

   Available since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

7. pMAL-SsoPCNA3

   Plasmid

   #107069

   Purpose

   Expresses S. solfataricus PCNA3 fused to MBP in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

   Insert

   S. solfataricus PCNA3
   

   Use

   Tags

   6xHis-thrombin cleavage site and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   tac promoter

   Available since

   March 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX6P1-GCN4cc-Erp2CT

   Plasmid

   #73923

   Purpose

   GST fusion- coiled coil- Erp2 cytoplasmic tail

   Depositor

   Blanche Schwappach

   Article

   Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

   Insert

   Erp2 CT
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Dec. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX6P1-GCN4cc-Erp1CT

   Plasmid

   #73921

   Purpose

   GST fusion- coiled coil- Erp1 cytoplasmic tail

   Depositor

   Blanche Schwappach

   Article

   Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

   Insert

   Erp1 CT
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Dec. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Ubiquitin K6C/G76C

    Plasmid

    #86584

    Purpose

    Bacterial expression of Ubiquitin K6C/G76C

    Depositor

    Sharon Rozovsky

    Article

    Liu et al J Am Chem Soc. 2017 Feb 10. doi: 10.1021

    Insert

    Ubiquitin (UBC Human)
    

    Use

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    TEV site; K6C/G76C

    Promoter

    Tac

    Available since

    Feb. 24, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. Ubiquitin K48C/G76C

    Plasmid

    #86585

    Purpose

    Bacterial expression of Ubiquitin K48C/G76C

    Depositor

    Sharon Rozovsky

    Article

    Liu et al J Am Chem Soc. 2017 Feb 10. doi: 10.1021

    Insert

    Ubiquitin (UBC Human)
    

    Use

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    TEV site; K48C/G76C

    Promoter

    Tac

    Available since

    Feb. 24, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. Ubiquitin K63C/G76C

    Plasmid

    #86586

    Purpose

    Bacterial expression of Ubiquitin K63C/G76C

    Depositor

    Sharon Rozovsky

    Article

    Liu et al J Am Chem Soc. 2017 Feb 10. doi: 10.1021

    Insert

    Ubiquitin (UBC Human)
    

    Use

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    TEV site; K63C/G76C

    Promoter

    Tac

    Available since

    Feb. 24, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. AEB-T4-2

    Plasmid

    #63855

    Purpose

    This pFTV1 vector contains a thyroxine binding riboswitch, controlling the translation initaition rate of luciferase gene.

    Depositor

    Howard Salis

    Article

    Espah Borujeni et al Nucleic Acids Res. 2015 Nov 30. pii: gkv

    Insert

    T4-2 thyroxine riboswitch
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    tac

    Available since

    Feb. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AEB-TMR7pUC19

    Plasmid

    #63857

    Purpose

    This pUC19 vector contains a tetramethylrosamine (TMR) binding riboswitch, controlling the translation initaition rate of luciferase gene.

    Depositor

    Howard Salis

    Article

    Espah Borujeni et al Nucleic Acids Res. 2015 Nov 30. pii: gkv

    Insert

    TMR7 TMR riboswitch
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    tac

    Available since

    Feb. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pGEX-4T-1-p19-T7

    Plasmid

    #46306

    Depositor

    Judy Lieberman

    Article

    Huang et al Nat Biotechnol. 2013 Apr;31(4):350-6. do

    Inserts

    p19
    DNA-1 site
    linker
    DNA-2 site
    

    Use

    Pro-sirna

    Tags

    6XHis and GST

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    July 23, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pCMV5-ActRIIA

    Plasmid

    #11754

    Depositor

    Jeff Wrana

    Article

    Macias-Silva et al J Biol Chem. 1998 Oct 2. 273(40):25628-3

    Insert

    Activin receptor IIA (Acvr2a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 14, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. SAE2-mGarnet

    Plasmid

    #175240

    Purpose

    Bacterial expression and purification, combined with SAE1 to form the E1 enzyme heterodimer

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    UBA2 (UBA2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDEST-hisMBP-AsCpf1-EC

    Plasmid

    #79007

    Purpose

    Expressing his-MBP tagged AsCpf1 (E.coli codon optimized)

    Depositor

    Jin-Soo Kim

    Article

    Hur et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

    Insert

    AsCpf1
    

    Use

    Tags

    6xHis-MBP-NLS and NLS-HA

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MBP-TEVcs(ENLYFQ/G)-His6-uTEV2Δ(220-242)-R5

    Plasmid

    #135456

    Purpose

    Bacterial expression of uTEV2Δ protease

    Depositor

    Alice Ting

    Article

    Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

    Insert

    MBP-TEVcs(ENLYFQ/G)-His6-TEVΔ(220-242)-R5
    

    Use

    Tags

    MBP, His6

    Expression

    Bacterial

    Mutation

    S219V mutation improves stability, T30A/S153N mut…

    Promoter

    tac promoter

    Available since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. His-CL7-2NLS-SpyCas9(dS)-A22p3star

    Plasmid

    #222970

    Purpose

    Bacterial expression of A22p-fused SpyCas9 construct

    Depositor

    Jennifer Doudna

    Article

    Chen et al Nat Commun. 2024 Feb 26;15(1):1727. doi:

    Insert

    His-CL7-2NLS-SpyCas9(dS)-A22p3
    

    Use

    CRISPR

    Tags

    CL7 and His

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge