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pENTR221 UBR5 ∆UBR

Plasmid

#81063

Purpose

Gateway entry vector encoding full length UBR5 W1235L (∆UBR)

Depositor

Darren Saunders

Publication

Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

Insert

Ubiquitin protein ligase E3 component N-recognin 5 (UBR5) ∆UBR (UBR5 Human)

Use

Cloning

Mutation

W1235L (bp g3704t)

Promoter

N/A

Available since

Jan. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR221 UBR5 ∆HECT

Plasmid

#81065

Purpose

Gateway entry vector encoding full length UBR5 C2768A (∆HECT)

Depositor

Darren Saunders

Publication

Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

Insert

Ubiquitin protein ligase E3 component N-recognin 5 (UBR5) ∆HECT (UBR5 Human)

Use

Cloning

Mutation

C2768A (bp t8302g and g8303c)

Promoter

N/A

Available since

Jan. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pLck-mVenus-C1

Plasmid

#84337

Purpose

Expresses a mVenus tagged with a membrane localization sequence

Depositor

Dorus Gadella

Publication

van Unen et al BMC Cell Biol. 2018 Jun 7;19(1):6. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Lck (MGCVCSSNPELPVAT)

Expression

Mammalian

Promoter

CMV

Available since

Dec. 12, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pDONR221-TAF15 Stop

Plasmid

#84895

Purpose

DONOR vector for Gateway cloning of TAF15

Depositor

Aaron Gitler

Publication

Couthouis et al Proc Natl Acad Sci U S A. 2011 Dec 27;10

Insert

TAF15 (TAF15 Human)

Use

Gateway Cloning

Promoter

none

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR221-EWSR1 Stop

Plasmid

#84887

Purpose

DONOR vector for Gateway cloning of EWSR1

Depositor

Aaron Gitler

Publication

Couthouis et al Hum Mol Genet. 2012 Jul 1;21(13):2899-91

Insert

EWSR1 (EWSR1 Human)

Use

Gateway Cloning

Promoter

none

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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negActinin-sstFRET-GR

Plasmid

#83417

Purpose

FRET-based actinin tension sensor (negative control)

Depositor

Tetsuya Kitaguchi

Publication

Wang et al J Biomech Sci Eng (2016). Vol.11, No.4

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

negActinin-sstFRET

Expression

Mammalian

Promoter

CMV

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only
pGWB445

Plasmid

#74831

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with eCFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Publication

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pME-FlEx switch

Plasmid

#82406

Purpose

Gateway-compatible FlEx cassette containing two pairs of heterotypic, antiparallel loxP-type recombination sites

Depositor

Judith Eisen

Publication

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Type

Empty backbone

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pGrDL_SPb

Plasmid

#83205

Purpose

receives miRNA target sequences for testing using the dual luciferase assay system

Depositor

Peer Schenk

Publication

Moyle et al Front Plant Sci. 2017 Sep 20;8:1631. doi

Insert

Tomato ACTIN promoter

Expression

Plant

Promoter

Tomato ACTIN

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p3E-P2A-CFP no-pA

Plasmid

#80827

Purpose

P2A followed by CFP without pA

Depositor

Philip Washbourne

Publication

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

p3E-P2A-CFP no pA

Expression

Mammalian

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pME-GFP-P2A

Plasmid

#80807

Purpose

GFP without stop codon followed by porcine teschovirus-1 2A (P2A) "self-cleaving" peptide

Depositor

Philip Washbourne

Publication

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

GFP-P2A

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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SPS-6803

Plasmid

#80458

Purpose

IPTG inducible expressoin of sucrose phosphate synthase derived from Synechocystis sp. PCC6803

Depositor

Daniel Ducat

Publication

Abramson et al Plant Cell Physiol. 2016 Oct 13. pii: pc

Insert

Sucrose phosphate synthase

Expression

Bacterial

Available since

Oct. 25, 2016

Availability

Academic Institutions and Nonprofits only

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p3E-mTagBFP-CAAX

Plasmid

#75176

Purpose

Membrane targeted TagBFP (blue) fluorophore with stop codon for C-terminal fusions

Depositor

Nicholas Cole

Publication

Don et al Zebrafish. 2016 Sep 15.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mTagBFP-CAAX

Use

Three prime entry vector for multisite gateway th…

Promoter

n/a

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pECT3

Plasmid

#75450

Purpose

Plasmid that serves as the PCR template to create genomic pdt3 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate

Depositor

James Collins

Publication

Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

Insert

pdt3

Expression

Bacterial

Promoter

none

Available since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSMVP-Cas9C

Plasmid

#80939

Purpose

Plasmid that expresses Cas9C in mammalian cells.

Depositor

George Church

Publication

Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cas9C

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pGWB440

Plasmid

#74826

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with eYFP (no promoter).

Depositor

Tsuyoshi Nakagawa

Publication

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available since

Sept. 29, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEGFP-N1-mt-ro2GFP

Plasmid

#82408

Purpose

Expression of redox sensitive GFP with a mitochondrial targeting sequence in mammalian cells

Depositor

S. James Remington

Publication

Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ro2GFP

Tags

leader sequence of the E1α subunit of pyruvate de…

Expression

Mammalian

Mutation

C48S/F64L/S147C/Q204C

Promoter

CMV

Available since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDrVSP(C302S)-IRES2-EGFP

Plasmid

#80331

Purpose

Expresses Danio rerio VSP (without phosphatase activity) with separated EGFP in mammalian cell

Depositor

Yasushi Okamura

Publication

Hossain et al J Biol Chem. 2008 Jun 27;283(26):18248-5

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Daino rerio voltage-sensing phosphatase (tpte Zebrafish)

Expression

Mammalian

Mutation

C302S

Promoter

CMV

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCOLA-T7-WspR

Plasmid

#79162

Purpose

Expresses WspR WT under a T7 promoter

Depositor

Ming Hammond

Publication

Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

Insert

WspR (wspR P. areuginosa)

Expression

Bacterial

Promoter

T7

Available since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pTKEI-Mal-B2

Plasmid

#79756

Purpose

Single fluorescent protein biosensor for maltose

Depositor

David Savage

Publication

Nadler et al Nat Commun. 2016 Jul 29;7:12266. doi: 10

Insert

Mal-B2

Tags

6xHis

Expression

Bacterial

Promoter

pTrc

Available since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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